Ultrasensitive detection of the β-adrenergic agonist brombuterol by a SERS-based lateral flow immunochromatographic assay using flower-like gold-silver core-shell nanoparticles

Fu, Xinghu; Chu, Yanxin; Zhao, Kang; Li, Jianguo; Deng, Anping

doi:10.1007/s00604-017-2178-3

Cited by 61 publications

(23 citation statements)

References 33 publications

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“…SERS signals are based on the increase of optical absorption for reporter molecules by orders of magnitude after their immobilization on the surface of nanoparticles. The possibility of such highly sensitive analyses is demonstrated in the works of Sanchez-Purra et al [110], Fu et al [111], and Marks et al [112]. Clarke et al [113] described the combination of SERS registration with rapid vertical flow technology as an additional means of increasing sensitivity.…”

Section: Proper Response For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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Section: Proper Response For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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“…For these reasons, SERS has been widely used for the detection of a wide range of analytes, from big biomolecules to small inorganic molecules . For the detection of small molecules, as small molecules are too small to combine to antigens, competitive indirect methods were usually used . In the process of ICA‐SERS, all the steps are identical to those of ICA, except that the gold colloid used as labels in ICA also work as the substrate for SERS in ICA‐SERS.…”

Section: Introductionmentioning

confidence: 99%

“…[15,16] For the detection of small molecules, as small molecules are too small to combine to antigens, competitive indirect methods were usually used. [17,18] In the process of ICA-SERS, all the steps are identical to those of ICA, except that the gold colloid used as labels in ICA also work as the substrate for SERS in ICA-SERS. Immunoprobes captured by the coating antigen on the test line of ICA strip can be used to represent the content of analyte and would be measured by SERS for quantitative analysis.…”

Section: Introductionmentioning

confidence: 99%

Detection of saliva morphine using surface‐enhanced Raman spectroscopy combined with immunochromatographic assay

Yang

et al. 2020

J Raman Spectroscopy

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An immunochromatographic assay (ICA) based on surface‐enhanced Raman scattering (SERS) method was developed for the detection of morphine in this study. Indirect competitive immunoadsorption was adopted in the ICA due to the small molecular weight of morphine. Antibody modified gold nanoparticles in the ICA were labeled with Raman reporters and were used as the SERS enhancement substrate. By measuring the feature peaks of Raman reporter MBA at 1,078 cm−1, a quantitative estimation of morphine can be obtained. The limit of detection of this ICA‐SERS method was 2.4 × 10−4 ng/ml for morphine, which was four orders of magnitude lower than the visual method. This study demonstrated that the proposed ICA‐SERS method is a good candidate for the rapid and accurate detection of illicit drugs in saliva. All the analyses in this paper were carried out in a Microsoft (MS) Excel platform using embedded R functions through Excel BERT add‐in.

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“…18,19 SERS has gained a lot of attention on account of its non-destructivity, high selectivity, ultrasensitivity, reliability and fast operation in testing chemical and/or biological molecules in trace amounts. 20,21 For SERSbased SAL detection, a principal challenge is the low binding affinity of these phenolic molecules on silver or gold surfaces, 22,23 resulting in poor SERS signals when the analytes are mixed with SERS-active nanoparticles directly (Fig. S1 †).…”

Section: Introductionmentioning

confidence: 99%