Ultrasensitive detection of trypsin in serum via nanochannel device

Abstract

A highly sensitive trypsin sensing system in serum was developed by using an anodic alumina oxide (AAO)-based, trypsin substrate-decorated hybrid ion permeation membrane. Owing to the trypsin-triggered peptide hydrolyzation reaction, the surface electrical feature of the peptide-decorated hybrid ion membrane changed. The electric double layer effect reduces the effective ion current diameter in the AAO nano unit, so that the ion current rectification ratio will be enhanced, realizing the quantitative detection of trypsin. The lowest detection concentration can be achieved as low as 0.1 pM. This method is no need for sample prepreparation, easy to operate, highly sensitive, and also applicable to other enzyme evaluation systems by changing corresponding substrates. This study provides a new idea for selective measurements of proteases in complex biological samples.

The Role of Nanomaterials in the Diagnosis and Treatment of Acute Pancreatitis

“…Самая низкая концентрация обнаружения трипсина может быть достигнута в 0,1 пM (пмоль/л). Кроме этого, электрохимический датчик можно использовать и в других системах оценки ферментов путем замены соответствующих субстратов [26].…”

Application of nanotechnology in the diagnosis and treatment of acute pancreatitis

“…Based on the change in the electrical properties of the peptide-modied hybrid ion membrane surface aer a trypsininitiated peptide hydrolysis reaction and the electric double layer effect reducing the effective ion current diameter within the AAO nanocells and improving the ion current rectication ratio, the minimum quantitative detection of trypsin concentration can reach 0.1 pM. 60 Nanozymes are a new class of enzymes with biocatalytic activity that also retain the unique properties of nanomaterials, and have become a research hotspot in the biomedical eld in recent years. 61 An oxidase-like nanozyme, citrate-capped platinum nanoparticles (Cit-Pt NPs), was designed by Lin et al Using protamine as an aggregation promoter for Cit-Pt NPs and a specic hydrolytic substrate for trypsin to enhance the oxidase activity of Cit-Pt NPs at pH 8, a one-step detection of trypsin can be achieved.…”

“…Based on the change in the electrical properties of the peptide-modified hybrid ion membrane surface after a trypsin-initiated peptide hydrolysis reaction and the electric double layer effect reducing the effective ion current diameter within the AAO nanocells and improving the ion current rectification ratio, the minimum quantitative detection of trypsin concentration can reach 0.1 pM. 60 …”

Peptide Assembled in a Nano-confined Space as a Molecular Rectifier for the Availability of Ionic Current Modulation

“…Peptide-containing elements with specific properties are increasingly attractive for creating nanostructures, , because peptides exhibit the availabilities of easy modification, diversified assembly, and biofriendliness. − Peptide-containing elements can be embedded to play multifunctional roles through motif assembly. − In previous research, ion channel peptides and assemblies have been employed to study their influence on the modulation of ion channel properties. − In biomimetic channels, the combination or function of targets may alter the surface charge of the nanochannels, including the number and polarity of charges, giving rise to changes in ionic flux and even ion transport selectivity. − In addition, as a kind of natural biopolymer, the charge polarity of peptides with different isoelectric points (p I s) may vary in biological systems with different pHs and ion concentrations . Therefore, peptides with editable sequences could be used as nanoregulators to achieve polarity conversion in the surface of nanochannels regulated by thermodynamics and kinetics.…”