Ultrasensitive determination of serotonin in human urine by a two dimensional capillary isotachophoresis-capillary zone electrophoresis hyphenated with tandem mass spectrometry

Piešťanský, Juraj; Matušková, Michaela; Cizmarova, Ivana; Majerová, Petra; Mikuš, Peter

doi:10.1016/j.chroma.2021.462190

Cited by 19 publications

(18 citation statements)

References 60 publications

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“…Moreover, the aspect of volatility and low ion strength was crucial in the electrolyte system choice when employing MS as a detection technique. Based on our previous experiences with 2D CE-MS [33][34][35][36], we decided to test electrolyte systems composed of NH 4 Ac and acetic acid (HAc). It was found out that lower pH and higher concentration of the counter ion were favorable for removing a larger amount of Surine TM matrix constituents in the preseparation ITP step and for increasing S/N ratio of the analytes (see Supporting information Fig.…”

Section: Itp Separationmentioning

confidence: 99%

“…A free‐flow ITP in a microfluidic device coupled with MS was introduced recently too [31]. According to our best knowledge, there are only five articles dealing with a hyphenation of a hydrodynamically closed mode apparatus in a column‐coupled arrangement with MS [32–36]. Foret et al.…”

Section: Introductionmentioning

confidence: 99%

“…A free-flow ITP in a microfluidic device coupled with MS was introduced recently too [31]. According to our best knowledge, there are only five articles dealing with a hyphenation of a hydrodynamically closed mode apparatus in a column-coupled arrangement with MS [32][33][34][35][36]. Foret et al [32] demonstrated in their pioneering work an analytical potential of such instrumentation via an ITP-ITP-MS combination while our group [33][34][35][36] focused on an ITP-CZE-MS combination.…”

Section: Introductionmentioning

confidence: 99%

“…According to our best knowledge, there are only five articles dealing with a hyphenation of a hydrodynamically closed mode apparatus in a column-coupled arrangement with MS [32][33][34][35][36]. Foret et al [32] demonstrated in their pioneering work an analytical potential of such instrumentation via an ITP-ITP-MS combination while our group [33][34][35][36] focused on an ITP-CZE-MS combination. These advanced 2D CE-MS methods were applied for the separation of B vitamins [32], cytochrome C tryptic peptides [32], drugs (pheniramine, varenicline) and their metabolites [33][34][35], and serotonin [36].…”

Section: Introductionmentioning

confidence: 99%

“…Foret et al [32] demonstrated in their pioneering work an analytical potential of such instrumentation via an ITP-ITP-MS combination while our group [33][34][35][36] focused on an ITP-CZE-MS combination. These advanced 2D CE-MS methods were applied for the separation of B vitamins [32], cytochrome C tryptic peptides [32], drugs (pheniramine, varenicline) and their metabolites [33][34][35], and serotonin [36].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of 1D a 2D ITP‐MS performance parameters and application possibilities: Ultratrace determination of B vitamins in human urine

et al. 2021

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The possibility to investigate analytes at ultra-low concentration levels still remains a hot topic in bioanalysis. In this area, various preconcentration techniques are an integral part of analytical procedures. When applying electromigration separation techniques, an isotachophoresis has been advantageously employed many times for this purpose. To solve current biomedical tasks effectively, an advanced two-dimensional isotachophoretic instrument (in a hydrodynamically closed separation system with an enhanced sample load capacity) hyphenated with mass spectrometry (ITP-ITP-MS) has been proposed by Foret and coworkers. As a continuation, this work represents the first study dealing with a full validation of an ITP-ITP-MS method. In order to see the benefits of an online ITP sample pretreatment (preconcentration and clean-up) on the performance parameters, the developed 2D ITP-MS method was compared with a corresponding 1D ITP-MS method. Application potentialities of the compared methods were demonstrated via a determination of two B vitamins, namely thiamine and pyridoxine, in human urine samples. The developed 2D ITP-MS method showed its enhanced effectivity and usefulness for a routine biomedical use (here, a reliable screening of trace B vitamins in human urine without an offline sample preparation).

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Section: Itp Separationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of 1D a 2D ITP‐MS performance parameters and application possibilities: Ultratrace determination of B vitamins in human urine

et al. 2021

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A Comprehensive Review on Capillary Electrophoresis–Mass Spectrometry in Advancing Biomolecular Research

Pont,

Vergara‐Barberán,

Carrasco‐Correa

2024

Electrophoresis

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This review provides an in‐depth exploration of capillary electrophoresis–mass spectrometry (CE–MS) in biomolecular research from 2020 to 2024. CE–MS emerges as a versatile and powerful tool due to its numerous advantages, facilitating the analysis of various biomolecules, including proteins, peptides, oligonucleotides, and other metabolites, such as lipids, carbohydrates, or amines, among others. The review extends to various CE modes and interfaces for the CE–MS coupling, offering comprehensive insights into their applications within biomolecular research. Furthermore, it effectively summarizes the conditions employed in CE–MS while also addressing critical aspects such as sample preparation requirements. Despite its advantages, the review highlights a gap between discovery and practical implementation, underscoring the need for large‐scale validation and method standardization to fully realize the potential of CE–MS in biomolecular research.

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Current applications of capillary electrophoresis‐mass spectrometry for the analysis of biologically important analytes in urine (2017 to mid‐2021): A review

Helena

Voráčová

Řemínek

et al. 2021

J of Separation Science

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Capillary electrophoresis coupled online with mass detection is a modern tool for analyzing wide ranges of compounds in complex samples, including urine. Capillary electrophoresis with mass spectrometry allows the separation and identification of various analytes spanning from small ions to high molecular weight protein complexes. Similarly to the much more common liquid chromatographymass spectrometry techniques, the capillary electrophoresis separation reduces the complexity of the mixture of analytes entering the mass spectrometer resulting in reduced ion suppression and a more straightforward interpretation of the mass spectrometry data. This review summarizes capillary electrophoresis with mass spectrometry studies published between the years 2017 and 2021, aiming at the determination of various compounds excreted in urine. The properties of the urine, including its diagnostical and analytical features and chemical composition, are also discussed including general protocols for the urine sample preparation. The mechanism of the electrophoretic separation and the instrumentation for capillary electrophoresis with mass spectrometry coupling is also included. This review shows the potential of the capillary electrophoresis with mass spectrometry technique for the analyses of different kinds of analytes in a complex biological matrix. The discussed applications are divided into two main groups (capillary electrophoresis with mass spectrometry for the determination of drugs and drugs of abuse in urine and capillary electrophoresis with mass spectrometry for the studies of urinary metabolome).

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Ultrasensitive determination of serotonin in human urine by a two dimensional capillary isotachophoresis-capillary zone electrophoresis hyphenated with tandem mass spectrometry

Cited by 19 publications

References 60 publications

Comparison of 1D a 2D ITP‐MS performance parameters and application possibilities: Ultratrace determination of B vitamins in human urine

Comparison of 1D a 2D ITP‐MS performance parameters and application possibilities: Ultratrace determination of B vitamins in human urine

A Comprehensive Review on Capillary Electrophoresis–Mass Spectrometry in Advancing Biomolecular Research

Current applications of capillary electrophoresis‐mass spectrometry for the analysis of biologically important analytes in urine (2017 to mid‐2021): A review

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