Ultrasensitive electrochemical detection of CYFRA 21-1 via in-situ initiated ROP signal amplification strategy

Lü, Jing; Hao, Lulu; Yang, Fei; Liu, Yanju; Yang, Huai-Xia; Yan, Shuxun

doi:10.1016/j.aca.2021.338889

Cited by 16 publications

(5 citation statements)

References 25 publications

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“…During tumor metastasis, a large amount of Cyfra 21-1 is released into the extracellular fluid during the intermediate stage of epithelial cell apoptosis, resulting in increased Cyfra 21-1 levels in body fluids ( Sheard et al, 2002 ; Li et al, 2006 ; Chen et al, 2018 ). At present, there are many methods to detect Cyfra 21-1 in blood, with a detection threshold as low as 9.08 fg mL −1 ( Lu et al, 2021 ; Yang et al, 2021 ; Lv et al, 2022 ). Lei et al developed an ICS test based on fluorescent microspheres for the rapid and quantitative detection of urinary Cyfra 21-1, which is in use for the diagnosis and prognostic monitoring of bladder cancer, confirming the reliable application of immunochromatographic technology in detecting Cyfra 21-1 levels ( Lei et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Development of a Colloidal Gold Immunochromatographic Strip for Rapid Detection of Cyfra 21-1 in Lymph Node Metastasis of Thyroid Cancer

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Thyroid cancer is the most common endocrine tumor, and the rate of early lymph node metastasis may be as high as 60%. Currently, detection of lymph node metastasis of thyroid cancer during surgery is limited and time-consuming. Elevated levels of Cyfra 21-1, the proteolytic portion of cytokeratin, are associated with the metastasis and progression of thyroid cancer and are an effective biomarker for the prognosis and diagnosis of thyroid cancer. In this study, an immunochromatographic strip test based on colloidal gold nanoparticles was developed to semi-quantitatively detect the levels of Cyfra 21-1 in lymph nodes within 15 min. The standard (calibration) curve equation was Y = 0.003708 × X + 0.1101, and the detection limit was 0.55–1.14 ng mL−1. The strip did not detect other protein markers of epithelial cells at a concentration of 500 ng mL−1, including cytokeratin 8, cytokeratin 18, epithelial membrane antigen, and epidermal surface antigen. The ability of the strip to differentiate positive from negative metastasis in 40 lymph node specimens was 100% concordant with that of immunohistochemical staining for Cyfra 21-1. In an assessment of 20 lymph node specimens that had been determined by postoperative histopathology to be positive for lymph node metastasis and 20 specimens that were negative, the sensitivity and specificity of the strip were 100% and 95%, respectively. The sensitivity of the strip remained stable when stored at room temperature for 6 months. Together, these results indicated that although further testing using a larger sample size will be required, this immunochromatographic strip test may be useful for rapid intraoperative detection of thyroid cancer metastasis to lymph nodes.

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Section: Discussionmentioning

confidence: 99%

Development of a Colloidal Gold Immunochromatographic Strip for Rapid Detection of Cyfra 21-1 in Lymph Node Metastasis of Thyroid Cancer

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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“…The results showed that the SI-eRAFTbased amplification strategy held great promise in the sensitive analysis of biomolecules. Recently, an in situ initiated ring-opening polymerization signal amplification strategy was also integrated with an electrochemical biosensor for the detection of CYFRA 21-1, which is a specific biomarker for non-small cell lung cancer [94]. In addition to the covalent bond for the polymerization of signal molecules, a peptide can self-assemble into various stable nanostructures via non-covalent interaction including hydrogen bonding, hydrophobic interaction, electrostatic interaction, non-specific Van der Waals and π-π stacking.…”

Section: In Situ Assembly Of Small Molecules and Biomoleculesmentioning

confidence: 99%

“…The results showed that the SI-eRAFT-based amplification strategy held great promise in the sensitive analysis of biomolecules. Recently, an in situ initiated ring-opening polymerization signal amplification strategy was also integrated with an electrochemical biosensor for the detection of CYFRA 21-1, which is a specific biomarker for non-small cell lung cancer [94].…”

Section: In Situ Assembly Of Small Molecules and Biomoleculesmentioning

confidence: 99%

In Situ Assembly of Nanomaterials and Molecules for the Signal Enhancement of Electrochemical Biosensors

et al. 2021

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The physiochemical properties of nanomaterials have a close relationship with their status in solution. As a result of its better simplicity than that of pre-assembled aggregates, the in situ assembly of nanomaterials has been integrated into the design of electrochemical biosensors for the signal output and amplification. In this review, we highlight the significant progress in the in situ assembly of nanomaterials as the nanolabels for enhancing the performances of electrochemical biosensors. The works are discussed based on the difference in the interactions for the assembly of nanomaterials, including DNA hybridization, metal ion–ligand coordination, metal–thiol and boronate ester interactions, aptamer–target binding, electrostatic attraction, and streptavidin (SA)–biotin conjugate. We further expand the range of the assembly units from nanomaterials to small organic molecules and biomolecules, which endow the signal-amplified strategies with more potential applications.

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“…Compared to other methods, polymerization is a simple, effective and low-cost strategy. 28 Long-chain polymers have abundant reactive sites and groups, which can be modified by the addition of a mass of probe molecules, thereby realizing signal amplification and improving detection sensitivity. Among lots of polymerization reactions, atom transfer radical polymerization (ATRP) exhibits distinctive advantages, including a wide range of available monomers, mild reaction conditions, and controllable polymer structure and molecular weight, thus being a promising and preferred approach for signal amplification.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive and specific assessment of ochratoxin A in herbal medicines via activator regeneration by electron transfer ATRP

et al. 2022

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Ultrasensitive electrochemical detection of CYFRA 21-1 via in-situ initiated ROP signal amplification strategy

Cited by 16 publications

References 25 publications

Development of a Colloidal Gold Immunochromatographic Strip for Rapid Detection of Cyfra 21-1 in Lymph Node Metastasis of Thyroid Cancer

Development of a Colloidal Gold Immunochromatographic Strip for Rapid Detection of Cyfra 21-1 in Lymph Node Metastasis of Thyroid Cancer

In Situ Assembly of Nanomaterials and Molecules for the Signal Enhancement of Electrochemical Biosensors

Highly sensitive and specific assessment of ochratoxin A in herbal medicines via activator regeneration by electron transfer ATRP

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