Ultrasensitive electrochemical detection of Pb2+ based on rolling circle amplification and quantum dotstagging

Tang, Shengqiang; Tong, Ping; Li, Heng; Tang, Jing; Zhang, Lan

doi:10.1016/j.bios.2012.10.073

Cited by 104 publications

(43 citation statements)

References 28 publications

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“…With a large dynamic range (6 orders of magnitude), a detection limit down to 30 fM was obtained based on three times standard deviation of the blank, which is at least 500-fold lower than those of previously reported fluorescence methods. 22,[26][27][28][29] This sensitivity is also much higher than those of several electrochemical and electrochemiluminescence approaches 24,25,30,31 and is even comparable to the surface plasmon resonance-based biosensor. 32 The details are summarized in Table 1.…”

Section: Optimization Of Sensing Conditionsmentioning

confidence: 85%

“…This assay time and the high signal-to-noise ratio (18-fold) are superior to those of the previously reported methods based on cascade amplification. [21][22][23][24][25] …”

Section: Optimization Of Sensing Conditionsmentioning

confidence: 99%

“…This assay time and the high signal-to-noise ratio (18-fold) are superior to those of the previously reported methods based on cascade amplification. [21][22][23][24][25] Analytical performance for Pb 2+ detection based on ESQM For the detection of Pb 2+ , a DNAzyme (GR-5 DNAzyme) is involved in the proposed biosensor as a Pb 2+ recognition unit. GR-5 DNAzyme is a functionalized nucleic acid with catalytic activity, cleaving the Figure 2 The fluorescence responses of the proposed sensing system (a) and basic SDA system (b) in the presence of 10 nM SDA primer, with corresponding backgrounds without SDA primer (c, d), respectively.…”

Section: Optimization Of Sensing Conditionsmentioning

confidence: 99%

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Polymerase/nicking enzyme synergetic isothermal quadratic DNA machine and its application for one-step amplified biosensing of lead (II) ions at femtomole level and DNA methyltransferase

et al. 2014

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Herein, we propose a novel and universal biosensing platform based on a polymerase-nicking enzyme synergetic isothermal quadratic DNA machine (ESQM). This platform tactfully integrates two signal amplification modules, strand displacement amplification (SDA) and nicking enzyme signal amplification (NESA), into a one-step system. A bifunctional DNA probe with a stem-loop structure was designed to be partly complementary to the SDA product and digested substrate of NESA for bridging SDA and NESA. ESQM can be performed by using only the enzymes and buffer involved in the SDA module. In the presence of a target, this DNA machine is activated to afford a high quadratic amplified signal. Using Pb 2+ and DNA adenine methylation (Dam) methyltransferase (MTase) as analytes, a sensitive biosensing platform is demonstrated. Low detection limits of 30 fM Pb 2+ and 0.05 U ml − 1 Dam MTase were achieved within a short assay time (40 min), which were each superior to those of most previously reported methods. This DNA machine exhibited high selectivity for Pb 2+ . Furthermore, the successful detection of complex environmental water samples demonstrated the applicability of the proposed strategy in real samples, holding great potential for its application in environmental monitoring, biomedical research and clinical diagnosis. INTRODUCTIONSignal amplification technologies have a critical role in molecular biology research, environmental and food monitoring, forensic identification and clinical diagnosis. Since its creation in 1985, polymerase chain reaction has become the most well-known and widely used amplification technique. 1 However, this technique suffers from an intrinsic drawback, the requirement of precision thermal cycling between three different temperatures in a costly thermal cycler, limiting its popularization and application in point-of-care testing. On the other hand, various alternative isothermal amplification methods, such as rolling circle amplification (RCA), 2,3 strand displacement amplification (SDA), 4 loop-mediated isothermal amplification, 5,6 nicking enzyme signal amplification (NESA) 7-9 and the exponential amplification reaction (EXPAR) 10 have been proposed in the past two decades. These isothermal methods have been developed mainly based on some new findings in molecular biology concerning DNA synthesis and some accessory proteins together with their mimicking in vitro for nucleic acid amplification. Among these methods, EXPAR has been widely used, which is attributed to its rapid amplification kinetics (several minutes) and exceedingly high amplification efficiency (10 6 -to 10 9 -fold). For example, Ye's group 11 and Zhang's group 12,13 have proposed several interesting methods for the highly sensitive detection of microRNA and protein based on EXPAR, respectively. Nevertheless, the EXPAR-based methods involve the weaknesses of early phase non-specific background amplification resulting from the binding of the polymerase to the single-stranded template. 14 Conversely, the linear amplification ...

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Section: Optimization Of Sensing Conditionsmentioning

confidence: 85%

“…This assay time and the high signal-to-noise ratio (18-fold) are superior to those of the previously reported methods based on cascade amplification. [21][22][23][24][25] …”

Section: Optimization Of Sensing Conditionsmentioning

confidence: 99%

Section: Optimization Of Sensing Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Polymerase/nicking enzyme synergetic isothermal quadratic DNA machine and its application for one-step amplified biosensing of lead (II) ions at femtomole level and DNA methyltransferase

et al. 2014

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“…Both systems showed good selectivity against other divalent metal ions and thus hold great potential for the construction of a general DNAzyme-based sensing platform for the monitoring of other heavy metal ions. 60 Another strategy for Pb 2þ monitoring based on CdS QD modified indium tin oxide electrode and photoelectrochemical analysis was developed by De-Man et al 61 K þ -stabilized G-quadruplex DNAzyme accelerates oxidation of 4-chloro-1-naphthol by H 2 O 2 to yield the insoluble and insulating product benzo-4-chlorohexadienone on the photoelectrode, reducing the photocurrent intensity. The sensing strategy was based on the Pb 2þ -induced allosteric transition of G-quadruplex DNAzyme, which reduced the enzymatic biocatalytic precipitation on the CdS QDs photoelectrode.…”

Section: Dnazymesmentioning

confidence: 99%

Analytical strategies based on quantum dots for heavy metal ions detection

Vázquez‐González

Carrillo‐Carrión

2014

J. Biomed. Opt

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Abstract. Heavy metal contamination is one of the major concerns to human health because these substances are toxic and retained by the ecological system. Therefore, in recent years, there has been a pressing need for fast and reliable methods for the analysis of heavy metal ions in environmental and biological samples. Quantum dots (QDs) have facilitated the development of sensitive sensors over the past decade, due to their unique photophysical properties, versatile surface chemistry and ligand binding ability, and the possibility of the encapsulation in different materials or attachment to different functional materials, while retaining their native luminescence property. This paper comments on different sensing strategies with QD for the most toxic heavy metal ions (i.e., cadmium, Cd 2þ ; mercury, Hg 2þ ; and lead, Pb 2þ ). Finally, the challenges and outlook for the QD-based sensors for heavy metals ions are discussed.

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“…The setting up of various materials has widened the electrochemical achievements. For concrete illustration, polymers or biomaterials are regularly come together on inorganic nanomaterials to manufacture the highly responsive electrochemical sensors for heavy metal ions [9][10][11][12][13][14][15][16][17][18] .Because of the potential for the small analytical period, less engine capacity, valuable sensitivity and simple adaptability for in-situ extent 19 ,electrochemical detection methods using anchored complexes have attracted enormous attention in the detection of heavy metal ions. This review summons up the formulation and sensing means such as the molecularly imprinted polymers, ion-imprinted polymers, exploitation incentive responses of a deoxyribozyme (DNAzyme), fluorescence and complexes with conducting nation binders for heavy metal ions in 2015 and 2016are briefly explained.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Anchored Complexes as Electrodes for Sensing Heavy Metal Ions by Electrochemical Method

Jose¹,

Datta²,

Sreeja³

2017

Orient. J. Chem

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Anchored coordination complexes as electrochemical sensors play a significant role in the modern era. It is evident that this becomes a fact on account of their practical convenience. Furthermore, they have unlimited scope in ecological, therapeutic, experimental and biomedical applications. It has been observed that 165 such papers have reported on anchored complexes for electrochemical sensing during the past two years. While human vitality is rigorously threatened by heavy metal ions today, numerous trials are restrained for screening these in nature. This retrace highlights the electro analytical methods and the masterpiece contribution of anchored coordination complexes as electrochemical sensors for the identification of heavy metals such as indium, uranium, lead, beryllium, and mercury in 2015 and 2016.

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Ultrasensitive electrochemical detection of Pb2+ based on rolling circle amplification and quantum dotstagging

Cited by 104 publications

References 28 publications

Polymerase/nicking enzyme synergetic isothermal quadratic DNA machine and its application for one-step amplified biosensing of lead (II) ions at femtomole level and DNA methyltransferase

Polymerase/nicking enzyme synergetic isothermal quadratic DNA machine and its application for one-step amplified biosensing of lead (II) ions at femtomole level and DNA methyltransferase

Analytical strategies based on quantum dots for heavy metal ions detection

Fabrication of Anchored Complexes as Electrodes for Sensing Heavy Metal Ions by Electrochemical Method

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