Ultrasensitive electrochemical platform for the p53 gene <i>via</i> molecular beacon-mediated circular strand displacement and terminal deoxynucleotidyl transferase-mediated signal amplification strategy

Wang, Yali; Liu, Shuai; Zhang, Dongyou; Xiao, Qi; Huang, Shan

doi:10.1039/d2an01676a

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…2 It is reported that about 50% of the most commonly mutated genes in human cancer are wild-type p53 genes, which play a key role in cell cycle arrest, DNA repair, apoptosis, and tumor angiogenesis. 3 Moreover, the transcriptional regulation of some proteins in the human body is also related to p53 DNA. [4][5][6] p53 DNA is a veritable "genome guardian".…”

Section: Introductionmentioning

confidence: 99%

A functional nucleic acid-based fluorescence sensing platform based on DNA supersandwich nanowires and cation exchange reaction

Wang,

Guan,

Liu

et al. 2023

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Section: Introductionmentioning

confidence: 99%

A functional nucleic acid-based fluorescence sensing platform based on DNA supersandwich nanowires and cation exchange reaction

Wang,

Guan,

Liu

et al. 2023

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“…25 We further employed a unique strategy of utilizing terminal deoxynucleotidyl transferase (TdT) to block the 3 0 hydroxyl group of DNA. 26,27 It is known that TdT is a specialized DNA polymerase enzyme that catalyzes the template-independent addition of deoxynucleotides to the 3 0 hydroxyl end of DNA strands. In our case, using TdT, we tagged dideoxyadenosine-5 0triphosphate (ddATP) to the 3 0 hydroxyl moiety of the probe/ target and thereby ensured no free hydroxyl group at this site.…”

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confidence: 99%

Direct experimental evidence for the boronic acid-mediated bridging of DNA hybrids

et al. 2023

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Development of a Competitive Chemiluminescent Assay for Quantitative Determination of TP53 Fusion Protein Using Reagent Strips

Wang,

He,

2024

Appl Biochem Biotechnol

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Recent studies have shown that almost half of all cancers occur due to DNA damage. For the early diagnosis of cancer, a highly sensitized and swift identi cation for TP53 is needed since the corresponding TP53 protein is effectively recognized as "the guardian of the genome." To improve the detection sensitivity, numerous analytical methods were previously used for the determination of the TP53 protein, including denaturing high-performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA). Currently, immunochromatographic tests (ICTS) that are simple to use, stable over time, and show low interference are regarded as valuable tools for the quick screening of food and environmental monitoring along with clinical diagnosis. ICTS often have limited sensitivity even if a variety of novel reporters possessing optimum photostability and improved brightness are used as the signal-intensity reporters. Compared with N-(4-aminobutyl)-N-(ethylisoluminol) or luminol, a novel luminescent probe, 2',6'-diMethyl-4'-(N-succiniMidyloxycarbonyl) phenyl-10-sulfopropylacridiniuM-9carboxylate (NSP-DMAE-NHS) has achieved a much higher e ciency, improvement in the biosensor's performance, and ampli cation of the signal without causing any damage to the biomolecule in terms of its biochemical activity. In this study, the reagent strip method was initially used to detect TP53 fusion protein by combining the advantages of NSP-DMAE-NHS and immunochromatography. In our experiment, the control and study lines on the strips were immobilized through HRP-conjugated goat antirabbit IgG and TP53 antigen, respectively. The optimized concentration of the anti-TP53 antibody-NSP-DMAE-NHS immunoconjugates was then added to the TP53 antigen samples. After, the test strips were inserted and left in the aforementioned buffer solution for an additional 20 minutes. Finally, a lab made luminous measurement device was used to analyze the corresponding control and study lines on the strips. Under optimized conditions, this method was found to be ultrasensitive, with a wide range of linear responses from 0.0008 ng mL -1 to 1 µg mL -1 and a limit of detection of 0.0008 ng mL -1 (0.013 pM). Thus, a novel competitive chemiluminescent assay based on reagent strips was established for the determination of the TP53 fusion proteins. The strategy has potential applications for ultrasensitive detection in the early diagnosis of cancer.

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Ultrasensitive electrochemical platform for the p53 gene via molecular beacon-mediated circular strand displacement and terminal deoxynucleotidyl transferase-mediated signal amplification strategy

Abstract: An ultrasensitive electrochemical platform for p53 gene via MB-mediated CSD and TdT-mediated signal amplification strategy with detection limit of 0.018 fM.

Cited by 7 publications

References 36 publications

A functional nucleic acid-based fluorescence sensing platform based on DNA supersandwich nanowires and cation exchange reaction

A functional nucleic acid-based fluorescence sensing platform based on DNA supersandwich nanowires and cation exchange reaction

Direct experimental evidence for the boronic acid-mediated bridging of DNA hybrids

Development of a Competitive Chemiluminescent Assay for Quantitative Determination of TP53 Fusion Protein Using Reagent Strips

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