Ultrasensitive electrochemical strategy for NT-proBNP detection with gold nanochains and horseradish peroxidase complex amplification

Zhuo, Ying; Yi, Weijing; Lian, Wen-Bing; Yuan, Ruo; Chen, An; Hu, Chuanmin

doi:10.1016/j.bios.2010.09.033

Cited by 68 publications

(35 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, by labeling an electroactive substance or enzyme on an antigen or antibody, the concentration of the antigen or antibody in the sample can be indirectly measured by monitoring the current change caused the reaction of the substrate of the electroactive substance or the enzyme catalyzed reaction between the immobilized biomolecule and the biomolecule in the solution. In this mode, the resulting current signal of the antigen antibody binding reaction has been amplified using the markers such as enzymes (alkaline phosphatase [27,28], horseradish peroxidase (HRP) [29,30] or glucose oxidase [31,32]) or nanomaterials (Au nanoparticles (AuNPs) [33], graphene [34], carbon nanotubes (CNTs) [35,36] or silica nanoparticles [37,38]), which has allowed the detection sensitivity and reproducibility to be increased to a certain extent. Additionally, according to the enzyme-linked immunoassay (ELISA) principle, this kind of immunosensors can be further divided into two categories based on competition or sandwich methodologies (Figure 1) [39].…”

Section: Electrochemical Immunosensors With Different Signalsmentioning

confidence: 99%

Nanomaterials for Electrochemical Immunosensing

Pan

Yun

et al. 2017

Sensors

View full text Add to dashboard Cite

Electrochemical immunosensors resulting from a combination of the traditional immunoassay approach with modern biosensors and electrochemical analysis constitute a current research hotspot. They exhibit both the high selectivity characteristics of immunoassays and the high sensitivity of electrochemical analysis, along with other merits such as small volume, convenience, low cost, simple preparation, and real-time on-line detection, and have been widely used in the fields of environmental monitoring, medical clinical trials and food analysis. Notably, the rapid development of nanotechnology and the wide application of nanomaterials have provided new opportunities for the development of high-performance electrochemical immunosensors. Various nanomaterials with different properties can effectively solve issues such as the immobilization of biological recognition molecules, enrichment and concentration of trace analytes, and signal detection and amplification to further enhance the stability and sensitivity of the electrochemical immunoassay procedure. This review introduces the working principles and development of electrochemical immunosensors based on different signals, along with new achievements and progress related to electrochemical immunosensors in various fields. The importance of various types of nanomaterials for improving the performance of electrochemical immunosensor is also reviewed to provide a theoretical basis and guidance for the further development and application of nanomaterials in electrochemical immunosensors.

show abstract

Section: Electrochemical Immunosensors With Different Signalsmentioning

confidence: 99%

Nanomaterials for Electrochemical Immunosensing

Pan

Yun

et al. 2017

Sensors

View full text Add to dashboard Cite

show abstract

“…An ultrasensitive electrochemical strategy for NT-proBNP detection was developed using gold nanochains (AuNCs) and HRP complex amplification at a nanostructured gold functionalized CNTs composite. AuNCs enabled the efficient immobilization of biomolecules and acted as conductive centers to facilitate electron transfer [74]. The AuNCs were prepared by using l -ascorbic acid (AA) as a mediator and template.…”

Section: Electrochemical Immunosensors Involving Carbon Nanomaterimentioning

confidence: 99%

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

2016

View full text Add to dashboard Cite

Early diagnosis is often the key to successful patient treatment and survival. The identification of various disease signaling biomarkers which reliably reflect normal and disease states in humans in biological fluids explain the burgeoning research field in developing new methodologies able to determine the target biomarkers in complex biological samples with the required sensitivity and selectivity and in a simple and rapid way. The unique advantages offered by electrochemical sensors together with the availability of high affinity and specific bioreceptors and their great capabilities in terms of sensitivity and stability imparted by nanostructuring the electrode surface with different carbon nanomaterials have led to the development of new electrochemical biosensing strategies that have flourished as interesting alternatives to conventional methodologies for clinical diagnostics. This paper briefly reviews the advantages of using carbon nanostructures and their hybrid nanocomposites as electrode modifiers to construct efficient electrochemical sensing platforms for diagnosis. The review provides an updated overview of some selected examples involving attractive amplification and biosensing approaches which have been applied to the determination of relevant genetic and protein diagnostics biomarkers.

show abstract

“…New, simpler, cost-effective methods for BNP detection such as the immunosensors are being tested as candidates for the detection of NTproBNP. They detect NT-proBNP in serum such as the regeneration-free immunosensor with novel Fab fragment monoclonal antibodies that detects NT-proBNP from 0.04 to 2.5 ng/ml with a limit of 0.03 ng/ml (12) or in whole blood such as the combination of a microfluidic system with an immunoassay based on an electrochemical immunosensor with magnetic nanoparticles, biotin-avidin system (BAS) and fragment antigen binding (Fab) antibodies (13) or the electrochemical immunosensor based on the nanostructural gold and carbon nanotubes composite, gold nanochains (AuNCs) and horseradish peroxidase (HRP) complex labeled secondary antibodies (AuNCs-HRP-Ab(2)) (14).…”

Section: Biomarkers Of Myocyte Stress and Apoptosis In Hfmentioning

confidence: 99%