Ultrasensitive electrochemiluminescence biosensing platform for miRNA-21 and MUC1 detection based on dual catalytic hairpin assembly

Li, Jiyang; Liu, Jingju; Bi, Yanni; Sun, Mimi; Bai, Jing; Zhou, Ming

doi:10.1016/j.aca.2020.01.034

Cited by 43 publications

(15 citation statements)

References 54 publications

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“…It could offer more reference information for disease diagnosis. Similarly, Li et al 41 proposed an ECL biosensor with DCHA as the signal amplification for miRNA-21 and mucin 1 (MUC1) detection (Fig. 3E).…”

Section: Newly Developed Cha Strategiesmentioning

confidence: 95%

Recent advances of catalytic hairpin assembly and its application in bioimaging and biomedicine

et al. 2022

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Section: Newly Developed Cha Strategiesmentioning

confidence: 95%

Recent advances of catalytic hairpin assembly and its application in bioimaging and biomedicine

et al. 2022

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“…The dual-target detection of miRNA-21 and MUC1 based on a dual catalytic hairpin assembly was performed by Li and co-workers [ 119 ]. GCE was modified by Au nanoflowers to which hybridization probe 1 was immobilized to recognize miRNA-21.…”

Section: Nanomaterials/nanoparticles-based Electrochemical Biosensmentioning

confidence: 99%

“…The biosensor detected miRNA-21 with LOD of 11 aM and MUC1 with LOD of 0.40 fg/mL. When both analytes were spiked into human serum samples, a recovery index between 98% and 103% was obtained [ 119 ] (Approach 3).…”

Section: Nanomaterials/nanoparticles-based Electrochemical Biosensmentioning

confidence: 99%

Electrochemical Nanobiosensors for Detection of Breast Cancer Biomarkers

Gajdošová

Lorencová

Kasák

et al. 2020

Sensors

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This comprehensive review paper describes recent advances made in the field of electrochemical nanobiosensors for the detection of breast cancer (BC) biomarkers such as specific genes, microRNA, proteins, circulating tumor cells, BC cell lines, and exosomes or exosome-derived biomarkers. Besides the description of key functional characteristics of electrochemical nanobiosensors, the reader can find basic statistic information about BC incidence and mortality, breast pathology, and current clinically used BC biomarkers. The final part of the review is focused on challenges that need to be addressed in order to apply electrochemical nanobiosensors in a clinical practice.

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“…We describe the synthesis of the CNDs, following environmentally friendly procedures, and their application as co-reactants to develop the ECL biosensor. Although some sensitive ECL biosensors for miRNA have previously been reported [43][44][45][46], they require complex and time-consuming development and labeling steps. Therefore, the search for new and quick methods for determining this biomarker is of great interest.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanodot–based electrogenerated chemiluminescence biosensor for miRNA-21 detection

et al. 2021

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A simple carbon nanodot–based electrogenerated chemiluminescence biosensor is described for sensitive and selective detection of microRNA-21 (miRNA-21), a biomarker of several pathologies including cardiovascular diseases (CVDs). The photoluminescent carbon nanodots (CNDs) were obtained using a new synthesis method, simply by treating tiger nut milk in a microwave reactor. The synthesis is environmentally friendly, simple, and efficient. The optical properties and morphological characteristics of the CNDs were exhaustively investigated, confirming that they have oxygen and nitrogen functional groups on their surfaces and exhibit excitation-dependent fluorescence emission, as well as photostability. They act as co-reactant agents in the anodic electrochemiluminescence (ECL) of [Ru(bpy)3]2+, producing different signals for the probe (single-stranded DNA) and the hybridized target (double-stranded DNA). These results paved the way for the development of a sensitive ECL biosensor for the detection of miRNA-21. This was developed by immobilization of a thiolated oligonucleotide, fully complementary to the miRNA-21 sequence, on the disposable gold electrode. The target miRNA-21 was hybridized with the probe on the electrode surface, and the hybridization was detected by the enhancement of the [Ru(bpy)3]2+/DNA ECL signal using CNDs. The biosensor shows a linear response to miRNA-21 concentration up to 100.0 pM with a detection limit of 0.721 fM. The method does not require complex labeling steps, and has a rapid response. It was successfully used to detect miRNA-21 directly in serum samples from heart failure patients without previous RNA extraction neither amplification process. Graphical abstract

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Ultrasensitive electrochemiluminescence biosensing platform for miRNA-21 and MUC1 detection based on dual catalytic hairpin assembly

Cited by 43 publications

References 54 publications

Recent advances of catalytic hairpin assembly and its application in bioimaging and biomedicine

Recent advances of catalytic hairpin assembly and its application in bioimaging and biomedicine

Electrochemical Nanobiosensors for Detection of Breast Cancer Biomarkers

Carbon nanodot–based electrogenerated chemiluminescence biosensor for miRNA-21 detection

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