Ultrasensitive Magnetic Assisted Lateral Flow Immunoassay Based on Chiral Monoclonal Antibody against R-(−)-Salbutamol of Broad-Specificity for 38 β-Agonists Detection in Swine Urine and Pork

Abstract

Screening for ″zero tolerance″ β-agonists requires broad-specificity and sensitivity methods. Herein, R-(−)-salbutamol (SAL) is chirally separated and designed as a hapten, and a monoclonal antibody (mAb) was first prepared with an IC50 of 0.27 ng/mL, which can recognize 38 β-agonists simultaneously. The broad-specificity of chiral mAb was explored by molecular simulation technology. Magnetic nanoparticles (MNPs) were then synthesized and applied as a signal tracer to develop a lateral flow immunoassay (LFIA). The limits of detection of MNPs-LFIA for SAL in swine urine and pork were 0.05 and 0.09 μg/kg, which was (2–125)-fold lower than that of the reported LFIAs. The recoveries were between 95.8 and 116.7%, with the coefficient of variation from 2.7 to 15.4%. Parallel analysis of 44 samples by commercial ELISA kits confirmed the reliability. Therefore, our work not only provides a broad-specificity and ultrasensitive method for β-agonists but also suggests that chirality is the new general theory that guided the rational hapten design.

Epitope-Shared Hapten Enhancing Antibody Polyreactivity for Simultaneous Immunochromatography of Oxyphenisatin Adulterants

“…Recovery Test. The sample extraction efficiency was assessed through recovery test, which was carried out as described by Liang et al 21 For detailed procedures, please see the Supporting Information.…”

Authenticating Emergent Adulterant 6-Benzylaminopurine in Bean Sprouts: Virtual Hapten Similarity Enhanced Immunoassay

“…It is also noteworthy that the antibody prepared in this work provided the lower IC 50 compared to previous reports, 14,15 suggesting that the computer-assisted screening of reasonably designed haptens with high similarity to the target analytes in terms of ESP and some physicochemical parameters can induce high-affinity antibodies, which also contributes to enhancing the immunoassay. 33,34 3.5. Specificity.…”

Trace Immunosensing of Multiple Neonicotinoid Insecticides by a Novel Broad-Specific Antibody Obtained from a Rational Screening Strategy

“…Although using multiple antibodies have been successfully applied to develop MAIAs, a more economical alternative is to raise a broad-specificity antibody that is able to recognize a class of the target compounds. Broad-specificity immunoassays had been developed for antibiotics [ 10 ], mycotoxins [ 11 ], veterinary drugs [ 12 ] and pesticides [ 13 ], and most of them can recognize more than three compounds from the same chemical families.…”