Ultrasensitive strategy based on PtPd nanodendrite/nano-flower-like@GO signal amplification for the detection of long non-coding RNA

Liu, Fei; Xiang, Guiming; Jiang, Dongneng; Zhang, Liqun; Chen, Xuemei; Liu, Linlin; Luo, Fukang; Li, Yi; Liu, Chang; Pu, Ximing

doi:10.1016/j.bios.2015.06.021

Cited by 54 publications

(12 citation statements)

References 40 publications

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“…A relative standard deviation for three independent measurements was calculated to be < 5.0%, indicating the excellent reproducibility of the assay towards the quantification of HOTAIR lncRNA. Our femtomolar detection limit was comparable to previously reported electrochemical assays for lncRNA [28,29]. Our previously reported isothermal amplification-based method for electrochemical quantification of HOTAIR also offers similar LOD [30].…”

Section: Detection Of Hotair Lncrna In Spiked Buffer Samplessupporting

confidence: 81%

“…A biosensor with dual readout ability that couples naked-eye visualisation (colorimtric signal) with electrochemical readout is highly compatible for biomolecular sensing, particularly in poor resource environments, where naked-eye evaluation could be used as the first screening pass of the analyte. Over the past years, a few integrated colorimetric and electrochemical assay platforms have been demonstrated for RNA detection [28,29]. Our group has recently developed a simple naked-eye colourimetric and electrochemical assay based on isothermal amplification readout for HOTAIR detection [30].…”

Section: Introductionmentioning

confidence: 99%

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PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples

Soda

Umer

Kashaninejad

et al. 2020

Cancers

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Long non-coding RNA HOX transcript antisense intergenic RNA (HOTAIR) is one of the promising biomarkers that has widely been used in determining the stages of many cancers, including ovarian cancer. In cancer diagnostics, the two key analytical challenges for detecting long non-coding RNA biomarkers are i) the low concentration levels (nM to fM range) in which they are found and ii) the analytical method where broad dynamic range is required (four to six orders of magnitude) due to the large variation in expression levels for different HOTAIR RNAs. To meet these challenges, we report on a biosensing platform for the visual (colorimetric) estimation and subsequent electrochemical quantification of ovarian-cancer-specific HOTAIR using a screen-printed gold electrode (SPE-Au). Our assay utilizes a two-step strategy that involves (i) magnetic isolation and purification of target HOTAIR sequences and (ii) subsequent detection of isolated sequences using a sandwich hybridization coupled with horseradish peroxidase (HRP)-catalyzed reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. The assay achieved a detection limit of 1.0 fM HOTAIR in spiked buffer samples with excellent reproducibility (% RSD ≤ 5%, for n = 3). It was successfully applied to detect HOTAIR in cancer cell lines and a panel of plasma samples derived from patients with ovarian cancer. The analytical performance of the method was validated with standard RT-qPCR. We believe that the proof of concept assay reported here may find potential use in routine clinical settings for the screening of cancer-related lncRNAs.

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Section: Detection Of Hotair Lncrna In Spiked Buffer Samplessupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples

Soda

Umer

Kashaninejad

et al. 2020

Cancers

View full text Add to dashboard Cite

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“…Recently, researchers developed a novel biosensor for the detection of long non‐coding RNAs (lncRNAs) which is highly up‐regulated in liver cancer (HULC) . Their detection platform was based on Pt−Pd bimetallic nanodendrites/nanoflower‐like clusters on graphene oxide/Au/horseradish peroxidase (PtPd BND/BNF@GO/Au/HRP) to enhance the catalytic efficiency and sensitivity.…”

Section: Electrochemical Biosensors For Cancer Biomarkers Detectionmentioning

confidence: 99%

Electrochemical Biosensors for Cancer Biomarkers Detection: Recent Advances and Challenges

2016

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Biomarkers are described as characteristics that provide information about biological conditions whether normal or pathological. Detection of biomarkers at the earliest stage of the cancer is of utmost importance for clinical diagnosis. Electrochemical biosensors allow detecting the low levels of specific analytes in blood, urine or saliva and providing a sensitive approach for direct measurement for cancer biomarker detection. Moreover, the integration of electrochemical devices with nanomaterials, such as carbon nanotubes, gold and magnetic particles offer amplification and multiplexing capabilities for simultaneous measurements of cancer biomarkers very sensitively. This review summarizes the recent developments of electrochemical biosensors systems for the detection of cancer biomarkers with emphasis on voltammetric, amperometric and impedimetric biosensors. A special attention is paid to aptamers and miRNAs that are very promising for the ultra‐sensitive and specific cancer biomarker detection.

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“…A sensor using Pt/Pd for long non-coding RNA, known to upregulated hepatocellular carcinoma (HCC), was made by Liu et al [ 129 ] who used Pt/Pd bimetallic nanodendrites/nanoflower-like clusters on graphene oxide/Au/horseradish peroxidase to enhance the sensor’s catalytic efficiency and sensitivity for the detection of HCC. In a different approach that also used a bimetallic construct containing Au for the detection of malignant disease, Ye et al [ 131 ] created an iodide-responsive NP-based colorimetric platform, in which an aptamer-functionalized Cu/Au NP probe was used to quantitatively connect an iodide-catalyzed colorimetric assay with target recognition molecules to create an ultra-sensitive cancer cell detection method for human leukaemia CCRF-CEM cells.…”

Section: Biosensors—grouped By Target Analytementioning

confidence: 99%

Biosensors Incorporating Bimetallic Nanoparticles

2015

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This article presents a review of electrochemical bio-sensing for target analytes based on the use of electrocatalytic bimetallic nanoparticles (NPs), which can improve both the sensitivity and selectivity of biosensors. The review moves quickly from an introduction to the field of bio-sensing, to the importance of biosensors in today’s society, the nature of the electrochemical methods employed and the attendant problems encountered. The role of electrocatalysts is introduced with reference to the three generations of biosensors. The contributions made by previous workers using bimetallic constructs, grouped by target analyte, are then examined in detail; following which, the synthesis and characterization of the catalytic particles is examined prior to a summary of the current state of endeavor. Finally, some perspectives for the future of bimetallic NPs in biosensors are given.

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Ultrasensitive strategy based on PtPd nanodendrite/nano-flower-like@GO signal amplification for the detection of long non-coding RNA

Cited by 54 publications

References 40 publications

PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples

PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples

Electrochemical Biosensors for Cancer Biomarkers Detection: Recent Advances and Challenges

Biosensors Incorporating Bimetallic Nanoparticles

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