Ultrasonic-assisted enzymatic improvement of polyphenol content, antioxidant potential, and in vitro inhibitory effect on digestive enzymes of Miang extracts

Leangnim, Nalapat; Unban, Kridsada; Thangsunan, Patcharapong; Tateing, Suriya; Khanongnuch, Chartchai; Kanpiengjai, Apinun

doi:10.1016/j.ultsonch.2023.106351

Cited by 8 publications

(1 citation statement)

References 49 publications

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“…Miang is an ethnic fermented tea made from leaves of Camellia sinensis var. assamica and has been reported to possess strong antioxidant, anti-inflammatory and antimicrobial activities [6][7][8]. Kodchasee et al [9] and Unban et al [10] have extensively studied the two processes adopted in the production of fermented Miang products including nonfilamentous fungi growth based (NFP)-Miang and filamentous fungi growth based (FFP)-Miang respectively, where they attributed the bioactivities of these teas to tannins, phenolic acids, catechin and its derivatives.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activities of Miang extracts against selected pathogens and the potential of the tannin-free extracts in the growth inhibition of Streptococcus mutans

Abdullahi,

Unban,

Saenjum

et al. 2024

PLoS ONE

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Bacterial pathogens have remained a major public health concern for several decades. This study investigated the antibacterial activities of Miang extracts (at non-neutral and neutral pH) against Bacillus cereus TISTR 747, Escherichia coli ATCC 22595, Salmonella enterica serovar Typhimurium TISTR 292 and Streptococcus mutans DMST 18777. The potential of Polyvinylpolypyrrolidone (PVPP)-precipitated tannin-free Miang extracts in growth-inhibition of the cariogenic Streptococcus mutans DMST 18777 and its biofilms was also evaluated. The tannin-rich fermented extracts had the best bacterial growth inhibition against S. mutans DMST 18777 with an MIC of 0.29 and 0.72 mg/mL for nonfilamentous fungi (NFP) Miang and filamentous-fungi-processed (FFP) Miang respectively. This observed anti-streptococcal activity still remained after PVPP-mediated precipitation of bioactive tannins especially, in NFP and FFP Miang. Characterization of the PVPP-treated extracts using High performance liquid chromatography quadrupole-time of flight-mass spectrometry (HPLC-QToF-MS) analysis, also offered an insight into probable compound classes responsible for the activities. In addition, Crystal violet-staining also showed better IC50 values for NFP Miang (4.30 ± 0.66 mg/mL) and FFP Miang (12.73 ± 0.11 mg/mL) against S. mutans DMST 18777 biofilms in vitro. Homology modeling and molecular docking analysis using HPLC-MS identified ligands in tannin-free Miang supernatants, was performed against modelled S. mutans DMST 18777 sortase A enzyme. The in silico analysis suggested that the inhibition by NFP and FFP Miang might be attributed to the presence of ellagic acid, flavonoid aglycones, and glycosides. Thus, these Miang extracts could be optimized and explored as natural active pharmaceutical ingredients (NAPIs) for applications in oral hygienic products.

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