Ultrasonic excitation of a bubble near a rigid or deformable sphere: Implications for ultrasonically induced hemolysis

Gracewski, Sheryl M.; Miao, Hongyu; Dalecki, Diane

doi:10.1121/1.1858211

Cited by 37 publications

(26 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The collapse of the microbubbles near a solid or tissue boundary results in the formation of a microjet [13, 14], which will mechanically shred the cells or bacteria in the case of a biofilm. When producing inertial cavitation in the MHz frequency range, the mechanical destruction is so fine that it can split a cell in half.…”

Section: Introductionmentioning

confidence: 99%

Histotripsy Treatment of S. Aureus Biofilms on Surgical Mesh Samples Under Varying Pulse Durations

Bigelow

Thomas

et al. 2017

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

View full text Add to dashboard Cite

Prior studies demonstrated that histotripsy generated by high-intensity tone bursts to excite a bubble cloud adjacent to a medical implant can destroy the bacteria biofilm responsible for the infection. The goal of this study was to treat S. aureus biofilms on surgical mesh samples while varying the number of cycles in the tone burst to minimize collateral tissue damage while maximizing therapy effectiveness. S. aureus biofilms were grown on 1 cm square surgical mesh samples. The biofilms were then treated in vitro using a spherically focused transducer (1.1 MHz, 12.9 cm focal length, 12.7 cm diameter) using either a sham exposure or histotripsy pulses with tone burst durations of 3, 5, or 10 cycles (pulse repetition frequency of 333 Hz, peak compressional pressure of 150 MPa, peak rarefactional pressure of 17 MPa). After treatment, the number of colony forming units (CFUs) on the mesh and the surrounding gel was independently determined. The number of CFUs remaining on the mesh for the sham exposure (4.8±0.9-log10) (sample mean ± sample standard deviation-log10 from 15 observations) was statistically significantly different from the 3-cycle (1.9±1.5-log10), 5-cycle (2.2±1.1-log10), and 10-cycle exposures (1.0±1.5-log10) with an average reduction in the number of CFUs of 3.1-log10. The numbers of CFUs released into the gel for both the sham and exposure groups were the same within a bound of 0.86-log10, but this interval was too large to deduce the fate of the bacteria in the biofilm following the treatment.

show abstract

Section: Introductionmentioning

confidence: 99%

Histotripsy Treatment of S. Aureus Biofilms on Surgical Mesh Samples Under Varying Pulse Durations

Bigelow

Thomas

et al. 2017

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

View full text Add to dashboard Cite

show abstract

“…With a sufficient number of collapse cavitation events, cell membranes Low-frequency ultrasound increases outer membrane permeability of Pseudomonas aeruginosa can be stressed by high fluid shear rates, or damaged by the heat or free radicals (Piyasena et al, 2003;Riesz and Kondo, 1992). Furthermore, cavitation adjacent to a solid surface (such as a bacterium) generates stress on the cell membrane during bubble expansion; and then during contraction an asymmetric collapse propels a high velocity jet of liquid toward the surface (Brennen, 1995;Gracewski et al, 2005). There is an intensity threshold for the production of collapse cavitation, and below this threshold collapse cavitation is absent, but stable cavitation occurs readily.…”

mentioning

confidence: 99%

Low-frequency ultrasound increases outer membrane permeability of Pseudomonas aeruginosa

Runyan

Carmen

Beckstead

et al. 2006

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

Low-frequency ultrasound has been investigated as an adjuvant to antimicrobial therapy, targeted at both planktonic and biofilm (sessile) organisms. Our previous work showed that ultrasound (US) effectively enhances the bactericidal activity of certain antibiotics against planktonic cultures (Pitt et al., 1994;Rediske et al., 1999) and in vitro biofilms (Johnson et al., 1998;Qian et al., 1999) and in vivo biofilms (Carmen et al., 2004b(Carmen et al., , 2005Rediske et al., 2000) of gram-positive and gram-negative bacteria. Ultrasound was shown to increase the transport of antibiotics through biofilms (Carmen et al., 2004a) which could account for some (or all) of the enhanced antibiotic activity against insonated biofilms; but such a mechanism could not account for US-enhanced antibiotic activity in planktonic cultures which have no extensive exopolymer matrix to retard antibiotic transport.Because this ultrasonic enhancement of antibiotic activity operates on both planktonic and sessile bacteria, we posit that US does more than simply increase the transport of antibiotic to the cells; ultrasound is postulated to increase uptake of antibiotic into the cells by rendering the cell membrane more permeable to the antibiotic. To examine this postulate, we must first review how ultrasound interacts with cells.Bacterial cells are fairly transparent to ultrasound; that is, ultrasonic waves go right through cells with little absorption, scattering or other interaction. However, the pressure oscillations of ultrasound produce size oscillations in any gas bubbles in the liquid (Brennen, 1995). These bubbles range in size from approximately 1 mm to 100 mm in diameter (Brennen, 1995). The oscillations of bubbles, called cavitation, are generally divided into "stable" and "collapse" types of cavitation. Stable cavitation is the low intensity oscillation of the bubbles without complete collapse of the bubble, while collapse cavitation occurs at higher intensity levels and lower frequencies wherein these bubbles collapse and violently accelerate the fluid around them. During bubble collapse, adiabatic heating of the gas produces very high temperature, produces free radicals, generates very high liquid shear force, and generates a shock wave as the collapsing spherical wall slams into itself (Brennen, 1995). With a sufficient number of collapse cavitation events, cell membranes

show abstract

“…[13][14][15][16][17] Other investigations have focused on bubble motion near rigid and deformable, but also immovable spheres. 18,19 These latter studies have concentrated primarily on modeling the violent aspherical collapse of the bubble and have employed boundary integral or other numerical techniques. In contrast, here we present an analytical model valid for low amplitude spherical oscillations, but for which both the bubble and particle are free to translate.…”

Section: Introductionmentioning

confidence: 99%

Model of coupled pulsation and translation of a gas bubble and rigid particle

Hay

Hamilton

Ilinskii

et al. 2009

The Journal of the Acoustical Society of America

View full text Add to dashboard Cite

A model of the interaction of a spherical gas bubble and a rigid spherical particle is derived as a coupled system of second-order differential equations using Lagrangian mechanics. The model accounts for pulsation and translation of the bubble as well as translation of the particle in an infinite, incompressible liquid. The model derived here is accurate to order R 5 / d 5 , where R is a characteristic radius and d is the separation distance between the bubble and particle. This order is the minimum accuracy required to account for the interaction of the bubble and particle. Dependence on the size and density of the particle is demonstrated through numerical integration of the dynamical equations for both the free and forced response of the system. Numerical results are presented for models accurate to orders higher than R 5 / d 5 to demonstrate the consequences of truncating the equations at order R 5 / d 5 .

show abstract

Ultrasonic excitation of a bubble near a rigid or deformable sphere: Implications for ultrasonically induced hemolysis

Cited by 37 publications

References 37 publications

Histotripsy Treatment of S. Aureus Biofilms on Surgical Mesh Samples Under Varying Pulse Durations

Histotripsy Treatment of S. Aureus Biofilms on Surgical Mesh Samples Under Varying Pulse Durations

Low-frequency ultrasound increases outer membrane permeability of Pseudomonas aeruginosa

Model of coupled pulsation and translation of a gas bubble and rigid particle

Contact Info

Product

Resources

About