Ultrasonic Extraction of Fructans From the Tubers of Jerusalem Artichoke: Optimization of Conditions, Purification Methods, C-13nmr Spectroscopy of the Product

Karetkin, Boris; Panfilov, Victor; Baurin, D.V.; Шакир, И.В.

doi:10.5593/sgem2015/b61/s25.087

Cited by 3 publications

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“…To remove the colored impurities (polyphenolic components, furfural etc. ), the extracts were clarified with activated charcoal, followed by separation of the coal by vacuum filtration [ 21 ]. The clarified extract was concentrated using the Hei-Vap Advantage rotary evaporator (model 561−01110−00 with glass set G1, Heidolph, Germany) giving a total carbohydrate content of 150–200 g·L −1 .…”

Section: Methodsmentioning

confidence: 99%

A Study on the Synbiotic Composition of Bifidobacterium bifidum and Fructans from Arctium lappa Roots and Helianthus tuberosus Tubers against Staphylococcus aureus

et al. 2021

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A number of mechanisms have been proposed explaining probiotics and prebiotics benefit human health, in particular, probiotics have a suppression effect on pathogen growth that can be enhanced with the introduction of prebiotics. In vitro models enhanced with computational biology can be useful for selecting a composition with prebiotics from new plant sources with the greatest synergism. Water extracts from burdock root and Jerusalem artichoke tubers were purified by ultrafiltration and activated charcoal and concentrated on a rotary evaporator. Fructans were precipitated with various concentrations of ethanol. Bifidobacterium bifidum 8 VKPM AC−2136 and Staphylococcus aureus ATCC 43300 strains were applied to estimate the synbiotic effect. The growth of bifidobacteria and staphylococci in monocultures and cocultures in broths with glucose, commercial prebiotics, as well as isolated fructans were studied. The minimum inhibitory concentrations (MICs) of lactic and acetic acids for the Staphylococcus strain were determined. A quantitative model joining the formation of organic acids by probiotics as antagonism factors and the MICs of pathogens (as the measure of their inhibition) was tested in cocultures and showed a high predictive value (R2 ≥ 0.86). The synbiotic factor obtained from the model was calculated based on the experimental data and obtained constants. Fructans precipitated with 20% ethanol and Bifidobacterium bifidum have the greater synergism against Staphylococcus.

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Section: Methodsmentioning

confidence: 99%

A Study on the Synbiotic Composition of Bifidobacterium bifidum and Fructans from Arctium lappa Roots and Helianthus tuberosus Tubers against Staphylococcus aureus

et al. 2021

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Antagonistic activity of synbiotics: Response surface modeling of various factors

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Karetkin

Zhurikov

et al. 2022

Foods and Raw Materials

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Synbiotic compositions have a great potential for curing microbial intestinal infections. Novel targeted synbiotics are a promising field of the modern functional food industry. The present research assessed the effect of various fructan fractions, initial probiotic counts, and test strains on the antagonistic properties of synbiotics. The research involved powdered roots of Arctium lappa L. and strains of Bifidobacterium bifidum, Bacillus cereus, and Salmonella enterica. The experiment was based on the central composite rotatable design. A water extract of A. lappa roots was purified and concentrated. Fructan fractions were precipitated at various concentrations of ethanol, dried, and sub jected to carbon-13 nuclear magnetic resonance (13C-NMR) spectrometry. The bifidobacteria and the test strains were co-cultivated in the same medium that contained one of the fractions. Co-cultivation lasted during 10 h under the same conditions. The acid concentrations were determined by high-performance liquid chromatography to define the synbiotic factor. The obtained fructans were closer to commercial oligofructose in terms of the number and location of NMR peaks. However, they were between oligofructose and inulin in terms of signal intensity. The response surface analysis for bacilli showed that the minimal synbiotic factor value corresponded to the initial probiotic count of 7.69 log(CFU/mL) and the fructan fraction precipitated by 20% ethanol. The metabolites produced by the bacilli also affected their growth. The synbiotic factor response surface for the experiments with Salmonella transformed from parabolic to saddle shape as the initial test strain count increased. The minimal synbiotic factor value corresponded to the lowest precipitant concentration and the highest probiotic count. The research established a quantitative relationship between the fractional composition of fructans and the antagonistic activity of the synbiotic composition with bifidobacteria. It also revealed how the ratio of probiotic and pathogen counts affects the antagonism. The proposed approach can be extrapolated on other prebiotics and microbial strains in vivo.

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System analysis of the state and prospects for the development of the production of inulin (review)

Бызов¹

2022

Аграрная наука Евро-Северо-Востока

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The review informs on the results of the research of the application of inulin as a bioactive prebiotic in functional nutritional products, in cosmetic formulations and pharmaceuticals as the excipient of anticancer agents on the basis of the system analysis. The steady trend of the world growth of inulin production of 8-10 % a year has been noted. The aim of the research is to reveal and to analyze the effect of the system complex of inulin production in sequential chain from the inulin synthesis, its accumulation in plants and extraction. The Jerusalem artichoke is the most advanced kind of raw material which has the high crop yield of tubers up to 40 t/ha and inulin content up to 20 %. The sucrose and the synthesizing enzymes 1-SST and 1-FFT take an active part in the inulin biosynthesis as their genes can be edited with the purpose of changing the inulin content. At the Jerusalem artichoke breeding the most prospective trend is the cross-species hybridization drawing on the example of a topinsunflower with the crop yield of tubers of 40 t/ha. In the Jerusalem artichoke seed breeding the new methods of clonal micropropagation of tubers and its cultivation in the airhydroponic environment with the achievement of the inulin content no less than 20 % have been studied. At Jerusalem artichoke cultivation the breed is significative for the maximum crop yield of tubers with the row width of 90 cm and with the interval between tubers in a row – 30 cm. The least loss of the tubers weight and the inulin content in them under the long-term storage is achieved at the temperatures from -5 to 0 °C. The technology of the Jerusalem artichoke tubers processing into inulin is suggested as the system of partly optimized technological operations from peeling and washing the tubers to receiving the powdered inulin and the oligofructose syrup. The system complex is suggested in the form of the structural topological model combining 4 systems: «Selection and seed breeding», «Agritechnologies», «Harvesting and storage of tubers», «The tubers processing technologies» with the interrelations in the form of the technological requirements and the output data of every system and of the complex as a whole, which is the basis for the development of the agri-food technology of inulin from Jerusalem artichoke and other kinds of raw materials.

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Ultrasonic Extraction of Fructans From the Tubers of Jerusalem Artichoke: Optimization of Conditions, Purification Methods, C-13nmr Spectroscopy of the Product

Cited by 3 publications

References 0 publications

A Study on the Synbiotic Composition of Bifidobacterium bifidum and Fructans from Arctium lappa Roots and Helianthus tuberosus Tubers against Staphylococcus aureus

A Study on the Synbiotic Composition of Bifidobacterium bifidum and Fructans from Arctium lappa Roots and Helianthus tuberosus Tubers against Staphylococcus aureus

Antagonistic activity of synbiotics: Response surface modeling of various factors

System analysis of the state and prospects for the development of the production of inulin (review)

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