Polysaccharides from Inonotus obliquus exhibited many biological activities including antitumor, antioxidant, hypoglycemic, immune-stimulating effects, and so on. Ultrasonic treatment is now an usual proceeding technique for the extraction of some bioactive constituents. But there was no information about the effects of ultrasonic treatment on the physicochemical properties and DPPH radical scavenging activity of polysaccharides from I. obliquus until now. Ultrasonic technique was an effective method to extract bioactive polysaccharides from I. obliquus (UPS) according to this study.