Ultrasonic separation of suspended particles

Benes, E.; Gröschl, Martin; Nowotny, H.; Trampler, Felix; Keijzer, Timo; Böhm, Hannes R.; Radel, Stefan; Gherardini, Lisa; Hawkes, Jeremy J.; König, Ralf; Delouvroy, Ch.

doi:10.1109/ultsym.2001.991812

Cited by 48 publications

(38 citation statements)

References 22 publications

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“…Especially the breakdown of the spatial arrangement of cell agglomerates poses a problem as shown by Radel et al [42]. Air bubbles have been found to interfere with the separation process in the experiments described here to such an extent that the separation has to be performed with a gas-free suspension (Benes et al [43]). Since the influence of cell concentration has already been evaluated, the residual suspension parameters to be examined are oil concentration and product concentration.…”

Section: Influence Of Substrate and Product Concentrationsmentioning

confidence: 82%

Sophorolipids from Candida bombicola: Cell separation by ultrasonic particle manipulation

Palme

Comanescu

Stoineva

et al. 2010

Euro J Lipid Sci & Tech

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The yeast Candida bombicola ATCC 22214 is well-known to produce mixtures of glycolipids containing the sugar sophorose, the so-called sophorolipids, especially when cultivated on hydrophobic carbon sources as co-substrates. To improve cultivation efficiency, an integrated process was developed using ultrasound separation technology. Since this technology is new for use with C. bombicola, it was first characterized in batch experiments and afterwards implemented in an integrated production process. In this process, separation efficiencies of about 99% C. bombicola cells could be achieved, leading to 8 g/L of nearly cell-free sophorolipid product and a total amount of 73.8 g/L sophorolipids. Furthermore, a technical mixture of unusual branched fatty alcohols containing mainly 2-hexyl-1-decanol was used as co-substrate with glucose in a shake flask study. This resulted in the production of a new product, 1-O-b-glucopyranosyl-2-hexyldecanol, a molecule containing glucose as the sugar moiety and 2-hexyl-1-decanol as a branched hydrophobic side chain.

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Section: Influence Of Substrate and Product Concentrationsmentioning

confidence: 82%

Sophorolipids from Candida bombicola: Cell separation by ultrasonic particle manipulation

Palme

Comanescu

Stoineva

et al. 2010

Euro J Lipid Sci & Tech

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“…Devices based on this principle are exploited industrially as cell filters in biotechnology (Benes et al, 2001). This is possible due to the technique's non-destructiveness for biological cells -in contrast to the common ultrasonic cleaning devices which utilize the effect of cavitation at frequencies of a few 10 kHz (Wolf, 1995).…”

Section: Ultrasonic Particle Manipulationmentioning

confidence: 99%

Ultrasonic particle manipulation exploited in on-line infrared spectroscopy of (cell) suspensions

Radel

Schnöller

Lendl

et al. 2008

Elektrotech. Inftech.

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An ultrasonic standing wave field of 2 MHz was used to manipulate yeast cells in an on-line flow cell for Fourier transformed mid-infrared spectroscopy (FT-IR) purposes. Results show, that a significant decrease of the undesired formation of biofilms on the attenuated total reflection (ATR) surface was achieved. In addition the time resolution was more than doubled.Anwendung der Ultraschall-Teilchen-Manipulation fü r die Online-Infrarot-Spektroskopie von (Zell-)Suspensionen.Ein 2-MHz-Ultraschallstehwellenfeld wurde verwendet, um Hefezellen in einer Online-Durchflusszelle zu Zwecken der Fouriertransformierten Infrarotspektroskopie (FT-IR) anzuordnen. Die Resultate zeigen, dass eine signifikante Reduktion unerwü nschten Biofilms auf der totalreflektierenden (ATR) Oberflä che erreicht wurde. Darü ber hinaus konnte die Zeitauflö sung mehr als verdoppelt werden.

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“…In an acoustic standing-wave field, it is well known that objects smaller than the wavelength can be trapped at antinodes of the pressure field. Acoustic manipulation techniques have been investigated by several researchers [1][2][3][4]. Kozuka et al reported noncontact manipulation of particles in air by controlling the phase difference between two transducers [5].…”

Section: Introductionmentioning

confidence: 99%

High-speed noncontact ultrasonic transport of small objects using acoustic traveling wave field

Koyama

Nakamura

2010

Acoust. Sci. & Tech.

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Ultrasonic separation of suspended particles

Cited by 48 publications

References 22 publications

Sophorolipids from Candida bombicola: Cell separation by ultrasonic particle manipulation

Sophorolipids from Candida bombicola: Cell separation by ultrasonic particle manipulation

Ultrasonic particle manipulation exploited in on-line infrared spectroscopy of (cell) suspensions

High-speed noncontact ultrasonic transport of small objects using acoustic traveling wave field

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