Ultrasonically Induced Release from Nanosized Polymer Vesicles

Pangu, Gautam D.; Davis, K.; Bates, Frank S.; Hammer, Daniel A.

doi:10.1002/mabi.201000081

Cited by 31 publications

(23 citation statements)

References 54 publications

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“…Size measurements and the direct visualization of vesicles demonstrate that US does not completely rupture membranes into fragments but causes transient poration, as leakage from the core is governed by acoustic cavitation (8). The extent of leakage inversely depends on the membrane thickness and directly depends on the sonication time and intensity (7).…”

Section: Introductionmentioning

confidence: 99%

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Spiral computed tomography evaluation of rabbit VX2 hepatic tumors treated with 20 kHz ultrasound and microbubbles

Shen

Liu

et al. 2017

Oncology Letters

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Abstract. The aim of the present study was to explore the therapeutic effect of 20 kHz ultrasound (US) and microbubbles (MBs) on rabbit VX2 liver tumors by spiral computed tomography (CT) scanning. A total of 16 New Zealand rabbits with hepatic VX2 tumors were divided into four groups: Control, MB, low-frequency US and US + MB. The treatment effect was evaluated by spiral CT scanning prior to, during and following treatment (at 0 weeks and the end of 1 and 2 weeks). The tumor growth rate was recorded. The specimens of VX2 tumors were collected for histological examination and transmission electron microscopy (TEM). No significant differences were observed between tumor areas measured by CT and pathology after 2-week treatment (P>0.05). The mean tumor growth rates in the control, MB, US and US + MB groups after 2 weeks of treatment were 385±21, 353±12, 302±14 and 154±9%, respectively (P<0.05, US + MB vs. the other three groups). Hematoxylin and eosin staining in the US + MB group revealed coagulation necrosis, interstitial hemorrhage and intravascular thrombosis. In the control, MB and US groups, tumor cells exhibited clear nuclear hyperchromatism. TEM of US + MB revealed vascular endothelial cell wall rupture, widened endothelial cell gaps, interstitial erythrocyte leakage and microvascular thrombosis, while intact vascular endothelial cells and normal erythrocytes in the tumor vessels were observed in the control, MB and US groups. A combination of 20 kHz US and MBs may effectively inhibit rabbit VX2 tumors. Spiral CT scanning is an ideal method to evaluate the US treatment on rabbit tumors.

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Section: Introductionmentioning

confidence: 99%

“…US at 20 kHz can also increase membrane permeation (7). Size measurements and the direct visualization of vesicles demonstrate that US does not completely rupture membranes into fragments but causes transient poration, as leakage from the core is governed by acoustic cavitation (8).…”

Section: Introductionmentioning

confidence: 99%

Spiral computed tomography evaluation of rabbit VX2 hepatic tumors treated with 20 kHz ultrasound and microbubbles

Shen

Liu

et al. 2017

Oncology Letters

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“…In fact, some studies have already reported that both Fe 3 O 4 and γ-Fe 2 O 3 nanoparticles have dual enzyme-like activities, which means both intrinsic peroxidase-like activity in an acidic solution and a catalase-like activity at neutral pH (pH 7.4) [46,47]. Fan et al [48] reported that MNPs could catalyze H 2 O 2 to produce hydroxyl radicals in acidic lysosome-mimic conditions, but no hydroxyl radicals were observed in neutral cytosol-mimic conditions. Therefore, the ROS toxicity of MNPs may be uptake-route-dependent.…”

Section: Science China Materialsmentioning

confidence: 99%

Altering the response of intracellular reactive oxygen to magnetic nanoparticles using ultrasound and microbubbles

Yang

Cui

et al. 2015

Sci. China Mater.

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Engineered iron oxide magnetic nanoparticles (MNPs) are one of the most promising tools in nanomedicine-based diagnostics and therapy. However, increasing evidence suggests that their specific delivery efficiency and potential long-term cytotoxicity remain a great concern. In this study, using 12 nm γ-Fe2O3 MNPs, we investigated three types of uptake pathways for MNPs into HepG2 cells: (1) a conventional incubation endocytic pathway; (2) MNPs co-administrated with microbubbles under ultrasound exposure; and (3) ultrasound delivery of MNPs covalently coated on the surface of microbubbles. The delivery efficiency and intracellular distribution of MNPs were evaluated, and the cytotoxicity induced by reactive oxygen species (ROS) was studied in detail. The results show that MNPs can be delivered into the lysosomes via classical incubation endocytic internalization; however, microbubbles and ultrasound allow the MNPs to pass through the cell membrane and enter the cytosol via a non-internalizing uptake route much more evenly and efficiently. Further, these different delivery routes result in different ROS levels and antioxidant capacities, as well as intracellular glutathione peroxidase activity for HepG2 cells. Our data indicate that the microbubble-ultrasound treatment method can serve as an efficient cytosolic delivery strategy to minimize long-term cytotoxicity of MNPs.

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“…Concentrations of TFN inside vesicles at different time points were investigated following procedure described below: 1 mL of solution V1-TFN was collected, filtered through hydrophilic syringe filter, and washed 1 time with 1 mL of deionized water to remove excess of the drug and PEG-2000, and residue was redispersed in 2 mL of water and subjected to sonication for 30 min at RT to break down all vesicles and release TFN. The ability of ultrasound to induce localized and controlled drug release from liposomes, synthetic diblock copolymers, and polymersomes is well known phenomena [17,18]. Next, TFN was extracted from this solution with large volume of diisopropyl ether; solvent was evaporated and remaining TFN was redissolved in 2 mL of acetonitrile.…”

Section: Afm Lcfm Sem Fesem and Tem-samples Preparationmentioning

confidence: 99%

Organic Nanovesicular Cargoes for Sustained Drug Delivery: Synthesis, Vesicle Formation, Controlling “Pearling” States, and Terfenadine Loading/Release Studies

Botcha

Dulla

Reddy

et al. 2014

Journal of Nanotechnology

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"Sustained drug delivery systems" which are designed to accomplish long-lasting therapeutic effect are one of the challenging topics in the area of nanomedicine. We developed an innovative strategy to prepare nontoxic and polymer stabilized organic nanovesicles (diameter: 200 nm) from a novel bolaamphiphile, where two hydrogen bonding acetyl cytosine molecules connected to 4,4 -positions of the 2,6-bispyrazolylpyridine through two flexible octyne chains. The nanovesicles behave like biological membrane by spontaneously self-assembling into "pearl-like" chains and subsequently forming long nanotubes (diameter: 150 nm), which further develop into various types of network-junctions through self-organization. For drug loading and delivery applications, the nanovesicles were externally protected with biocompatible poly(ethyleneglycol)-2000 to prevent them from fusion and ensuing tube formation. Nontoxic nature of the nanovesicles was demonstrated by zebrafish teratogenicity assay. Biocompatible nanovesicles were loaded with "terfenadine" drug and successfully utilized to transport and release drug in sustained manner (up to 72 h) in zebrafish larvae, which is recognized as an emerging in vivo model system.

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Ultrasonically Induced Release from Nanosized Polymer Vesicles

Cited by 31 publications

References 54 publications

Spiral computed tomography evaluation of rabbit VX2 hepatic tumors treated with 20 kHz ultrasound and microbubbles

Spiral computed tomography evaluation of rabbit VX2 hepatic tumors treated with 20 kHz ultrasound and microbubbles

Altering the response of intracellular reactive oxygen to magnetic nanoparticles using ultrasound and microbubbles

Organic Nanovesicular Cargoes for Sustained Drug Delivery: Synthesis, Vesicle Formation, Controlling “Pearling” States, and Terfenadine Loading/Release Studies

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