Ultrasonically Mediated Drug Delivery

Saxena, Juhi; Sharma, Neha; Makoid, Michael C.; Banakar, Umesh V.

doi:10.1177/088532829300700306

Cited by 11 publications

(5 citation statements)

References 22 publications

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“…On the other hand, in vivo, not all the cells in the tumor are exposed to the TUS in the same level owing to the three-dimensional structure of the tissue and the depth of TUS wave penetration. 29,30 A third possible explanation involves the ability of TUS to create cavitation in vivo. It is suggested that cavitation is less likely to occur in vivo, particularly in the dense tissue of the tumor, where there is less space available for bubbles to oscillate 31 and also when using frequencies in the therapeutic range (X1 MHz).…”

Section: Discussionmentioning

confidence: 99%

Efficient transfection of tumors facilitated by long-term therapeutic ultrasound in combination with contrast agent: from in vitro to in vivo setting

Duvshani-Eshet

Machluf

2007

Cancer Gene Ther

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Therapeutic ultrasound (TUS) is a promising non-viral clinical approach for the delivery of genes. This study demonstrates the efficient delivery and localization of DNA in subcutaneous tumors facilitated by TUS application and examines the contribution of ultrasound contrast-agent (USCA) addition on transfection. The study addresses the importance of in vivo optimization when using long-term TUS and USCA based on data achieved in vitro. In vitro results showed that transfection of TrampC2 prostate cancer (Pca) cells using genes encoding for luciferase and green fluorescent protein was enhanced when DNA and Optison were added together and TUS was applied for 20 or 30 min. In vivo results showed that the highest transfection was achieved when Optison and DNA were co-injected intratumoraly, and TUS was applied for 20 min. Using Optison significantly increased protein distribution in the tumor. However, in vivo expression level was decreased by two and four fold at 7 and 14 days, respectively, post-TUS. The study establishes the potential of intratumoral delivery of DNA-Optison, followed by TUS as an effective, non-toxic, gene delivery method that could provide a safe, clinical alternative to current viral gene delivery approaches where short-term gene expression is needed.

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Section: Discussionmentioning

confidence: 99%

Efficient transfection of tumors facilitated by long-term therapeutic ultrasound in combination with contrast agent: from in vitro to in vivo setting

Duvshani-Eshet

Machluf

2007

Cancer Gene Ther

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“…aeruginosa, saturated fatty acids in LPS produce tight packing of the molecules, preventing even hydrophobic molecules from easily partitioning into, and diffusing through, the LPS leaflet. Kassan et al (1996) and Saxena et al (1993) showed that ultrasound can enhance drug penetration through cell membranes. Others have investigated ultrasound as a potential facilitator of the delivery and absorption of drugs, mostly through the human epidermis (Mitragotri et al 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Kassan et al . (1996 ) and Saxena et al . (1993 ) showed that ultrasound can enhance drug penetration through cell membranes.…”

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confidence: 96%

Reducing bacterial resistance to antibiotics with ultrasound

Rediske

Rapoport

Pitt

1999

Letters in Applied Microbiology

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The effect of erythromycin on planktonic cultures of Pseudomonas aeruginosa, with and without application of 70 kHz ultrasound, was studied. Ultrasound was applied at levels that had no inhibitory effect on cultures of Ps. aeruginosa. Ultrasound in combination with erythromycin reduced the viability of Ps. aeruginosa by 1–2 orders of magnitude compared with antibiotic alone, even at concentrations below the minimum inhibitory concentration (MIC). Electron‐spin resonance studies suggest that ultrasound induces uptake of antibiotic by perturbing or stressing the membrane. This application of ultrasound may be useful for expanding the number of drugs available for treating localized infections by rendering bacteria susceptible to normally ineffective antibiotics.

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“…In addition, the presence and absence of cooling systems, processing of membranes used, distance between skin and transducer, size of transducer, quantity and type of coupling medium used, and end point evaluation techniques all affect the sonophoretic skin permeation rates. [129,187] Phonophoretic research often suffers from poor calibration in terms of the amount of ultrasound energy emitted. [188,189] The problem is that as an ultrasound propagates away from its source, the beam area begins to expand after a certain critical distance.…”

Section: Scope Of Future Researchmentioning

confidence: 99%

Sonophoresis: recent advancements and future trends

Rao¹,

Saini

2009

Journal of Pharmacy and Pharmacology

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Objectives Use of ultrasound in therapeutics and drug delivery has gained importance in recent years, evident by the increase in patents filed and new commercial devices launched. The present review discusses new advancements in sonophoretic drug delivery in the last two decades, and highlights important challenges still to be met to make this technology of more use in the alleviation of diseases. Key findings Phonophoretic research often suffers from poor calibration in terms of the amount of ultrasound energy emitted, and therefore current research must focus on safety of exposure to ultrasound and miniaturization of devices in order to make this technology a commercial reality. More research is needed to identify the role of various parameters influencing sonophoresis so that the process can be optimized. Establishment of long-term safety issues, broadening the range of drugs that can be delivered through this system, and reduction in the cost of delivery are issues still to be addressed. Summary Sonophoresis (phonophoresis) has been shown to increase skin permeability to various low and high molecular weight drugs, including insulin and heparin. However, its therapeutic value is still being evaluated. Some obstacles in transdermal sonophoresis can be overcome by combination with other physical and chemical enhancement techniques. This review describes recent advancements in equipment and devices for phonophoresis, new formulations tried in sonophoresis, synergistic effects with techniques such as chemical enhancers, iontophoresis and electroporation, as well as the growing use of ultrasound in areas such as cancer therapy, cardiovascular disorders, temporary modification of the blood-brain barrier for delivery of imaging and therapeutic agents, hormone replacement therapy, sports medicine, gene therapy and nanotechnology. This review also lists patents pertaining to the formulations and techniques used in sonophoretic drug delivery.

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Ultrasonically Mediated Drug Delivery

Cited by 11 publications

References 22 publications

Efficient transfection of tumors facilitated by long-term therapeutic ultrasound in combination with contrast agent: from in vitro to in vivo setting

Efficient transfection of tumors facilitated by long-term therapeutic ultrasound in combination with contrast agent: from in vitro to in vivo setting

Reducing bacterial resistance to antibiotics with ultrasound

Sonophoresis: recent advancements and future trends

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