Ultrasonographic assessment of superficial digital flexor tendon (SDFT) core lesion treated with platelet rich plasma (PRP) in donkeys (Equus Asinus)

Abstract

A B S T R A C TThe aim of this study was ultrasonographic evaluation, subjective and objective or quantitative, of the SDFT core lesion in donkeys treated with intra lesional injection of PRP. Subjective evaluation was performed by assessment of fiber echogenicity score (FES) and fiber alignment score (FAS). Quantitative evaluation was performed by gray scale histogram analysis and measuring of core lesion cross section area (CSA). The present study was carried out on two groups (Group-I) 12 donkeys treated by intra-tendenious injection of freshly prepared (PRP) and (group-II) 7 donkeys as control group and subjected to intra-tendenious injection of normal saline (Placebo). Ultrasonographic assessment were performed before induction, 5 th , 15 th , 30 th , 45 th , 60 th and 90 th days post induction of core lesion. The PRP treated group revealed significant (p ≤ 0.05) improvement of FES and FAS at 15 th , 30 th , 60 th and 90 th days and at 30 th days post induction, respectively. Moreover, there was significant improvement of the core lesion CSA and theme an gray scale histogram during all time points to reach near to the normal value at the end of the study. The mean gray value of the PRP treated group was significant (p ≤ 0.05) higher than that of control group at all-time points of the experiment. The obtained subjective and quantitative ultrasonographic assessments in the present investigations proved that these parameters could be valuable in the evaluation of SDFT core lesions and monitoring the treatment of tendopathy in equines.

Ultra-Sonographic Evaluation and Growth Factors Measurement of Using Tendon Derived Hydrogel and Hyaluronic Acid in Tendon Repair in Rams

“…While, at the same period in the treated group with tendon derived hydrogel the sonogram was showed, the presence of hyperechoic areas at the site of the tendon defect, which Indicates the improvement of tendon echogenicity become faster in second group. This finding comes in agreement with Nezih et al, (42) and Mostafa et al,(43). Improvement in echogenicity and fiber alignment occurred during tendon repair reflects the presence of immature granulation tissue with active fibrogenesis and collagen production associated with increased in the acoustic density (43).…”

“…This finding comes in agreement with Nezih et al, (42) and Mostafa et al,(43). Improvement in echogenicity and fiber alignment occurred during tendon repair reflects the presence of immature granulation tissue with active fibrogenesis and collagen production associated with increased in the acoustic density (43).…”

Platelet Rich Plasma in the Treatment of Superficial Digital Flexor Tendon Core Lesion in Donkeys: Histopathological Assessment

“…Previous assessment of intralesional PRP treatment in the horses with tendinopathy showed early improvement of ultrasonographic features, reduction of lameness, advanced collagen fiber organization and successful return of horses to racing have been reported (Gebruek et al, 2016;Alzola et al, 2018). Similar observations have also been documented previously in donkeys after SDFT core lesions during PRP treatment (Mostafa et al 2015a and2015b).…”

Clinicopathological and Ultrasonographic Changes during Experimentally Induced Arthritis in Donkey

“…Quantitative assessment of joint space echogenicity was achieved according to (Mostafa, Al-Akraa et al 2016)by image brightness analysis (on gray scale units from 0 (black) to 255 (white)) by using dedicated software(Image J) to obtain the mean gray value of the analyzed image.…”