Ultrasound assessment of angiogenesis in a matrigel model in rats

Stieger, Susanne M.; Bloch, Susannah H.; Foreman, Oded; Wisner, Erik R.; Ferrara, Katherine W.; Dayton, Paul A.

doi:10.1016/j.ultrasmedbio.2005.12.008

Cited by 34 publications

(24 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A diffuse cellular infiltration, including a heterogeneous population of macrophages, lymphocytes and plasma cells, was seen in all plugs (Fig. 8A-H), which is consistent with a previous study that used Matrigel in rats [18]. Statistical analysis proved the rMSCs CM to be the most potent to increase capillary and arteriolar density, but this CM had less effect on myofibroblast recruitment into the Matrigel plugs (Fig.…”

Section: Rahbarghazi Et Alsupporting

confidence: 90%

Juxtacrine and Paracrine Interactions of Rat Marrow-Derived Mesenchymal Stem Cells, Muscle-Derived Satellite Cells, and Neonatal Cardiomyocytes with Endothelial Cells in Angiogenesis Dynamics

Rahbarghazi‬

Nassiri

Khazraiinia

et al. 2013

Stem Cells and Development

View full text Add to dashboard Cite

Research into angiogenesis has contributed to progress in the fast-moving field of regenerative medicine. Designing coculture systems is deemed a helpful method to understand the dynamic interaction of various cells involved in the angiogenesis process. We investigated the juxtacrine and paracrine interaction between 3 different cells, namely rat marrow-derived mesenchymal stem cells (rMSCs), rat muscle-derived satellite cells (rSCs), and rat neonatal cardiomyocytes (rCMs), and endothelial cells (ECs) during angiogenesis process. In vitro Matrigel angiogenesis assay was performed whereby ECs were monocultured or cocultured with rMSCs, rSCs, and rCMs or their conditioned media (CM). In addition, in vivo Matrigel plug assay for angiogenesis was conducted to assess the angiogenic potential of the rCM-, rMSC-, and rSC-derived CM. Our results demonstrated that the rMSCs, rSCs, and rCMs elongated along the EC tubules, whereas the rMSCs formed tube-like structures with sprouting tip cells, leading to improved angiogenesis in the coculture system. Moreover, the rMSC-and rSC-derived CM significantly improved angiogenesis tube formation on Matrigel, accelerated EC chemotaxis, and increased the arteriolar density, vascularization index, and vascularization flow index in the Matrigel plug in vivo. Western blotting showed that rMSCs secreted a high level of vascular endothelial growth factor, basic fibroblast growth factor, and stromal-derived factor-1-alpha. Tie2 is also shed from rMSCs. This study demonstrated that stem cells interact with ECs in the juxtacrine and paracrine manner during angiogenesis, and marrow MSCs have superior angiogenic properties.

show abstract

Section: Rahbarghazi Et Alsupporting

confidence: 90%

Juxtacrine and Paracrine Interactions of Rat Marrow-Derived Mesenchymal Stem Cells, Muscle-Derived Satellite Cells, and Neonatal Cardiomyocytes with Endothelial Cells in Angiogenesis Dynamics

Rahbarghazi‬

Nassiri

Khazraiinia

et al. 2013

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…These reports demonstrate not only a high diagnostic potential and reliability of CE-US, but also a high safety profile of US-CAs; in adults, anaphylactoid reactions have been reported at a low rate of less than 0.002% [53,54]. The established indications are oncological, traumatic or inflammatory lesions, assessment of vascular patency or pathology, tissue perfusion, echocardiography, intraoperative guidance and transplant queries [32,[55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70].…”

Section: Resultsmentioning

confidence: 99%

Application of a second-generation US contrast agent in infants and children—a European questionnaire-based survey

Riccabona

2012

Pediatr Radiol

100

View full text Add to dashboard Cite

Background No US contrast agent (US-CA) is currently licensed for use in children.Objective To survey the off-label use in children of a second-generation US-CA. Materials and methods Questionnaires were e-mailed to European paediatric radiologists, who were asked about their experience with the second-generation US-CA Sonovue® (Bracco, Milan, Italy). Number of examinations per indication and adverse effects were recorded. Examinations were categorised by intravenous or intracavitary use of US-CA. Results Out of 146 respondents, 88 stated that they did not perform contrast-enhanced US in children, but 36 of these (44%) would appreciate paediatric approval. Fortyfive centres reported 5,079 examinations in children (age mean: 2.9 years; range: birth-18 years, M/F: 1/ 2.8). The majority (4,131 [81%] in 29 centres) were intravesical applications. The minority (948 [19%] in 30 centres) were intravenous applications. No adverse effects had been recorded from intravesical use. Six minor adverse effects (skin reaction, unusual taste, hyperventilation) had been recorded after five intravenous studies (0.52%). Conclusion Responses suggest a favourable safety profile of this second-generation US-CA in children. It also demonstrates a demand for such US-CA from paediatric radiologists.

show abstract

“…Paprottka et al [54] wiesen nach, dass der CEUS der farbkodierten Duplexsonographie und dem Powerdoppler bei der Beurteilung der Tumorperfusion im Tiermodell deutlich überlegen ist. Stieger et al [55] zeigten die deutliche Verbesserung der Beurteilung der Tumorangiogenese mit dem CEUS bei Mammakarzinomen sowie eine Korrelation mit der Gefäßdichte in der Immunhistochemie auf. Weitere präklinische Studien belegen, dass der CEUS für die Bewertung der zeitlichen Entwicklung der Tumorangionese sowie für die frühzeitige Bewertung des Erfolgs einer antiangiogenen Therapie geeignet ist [56].…”

Section: Kontrastverstärkter Ultraschallunclassified