Ultrasound-assisted enzymatic preparation of fatty acid ethyl ester in deep eutectic solvent

Hao, Xiaohong; Suo, Hang; Zhang, Guanhua; Xu, Peixing; Gao, Xin; Du, Su

doi:10.1016/j.renene.2020.09.114

Cited by 18 publications

(12 citation statements)

References 23 publications

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“…The formed DES was placed in a vacuum drying oven at 60°C for 48 h, then cooled to room temperature. Finally, the prepared DES was sealed and stored in a dry place 17 …”

Section: Methodsmentioning

confidence: 99%

“…Except for the difference in alcohol‐to‐oil ratio, the other parameters were lipase dosage of 6% of oil weight, ultrasonic power of 60 W, and DES dosage of 50% of the oil weight. The alcohol oil ratio was 3:1 mmol/g under the ultrasonic system, and the alcohol oil ratio was 4:1 mmol/g under DES and ultrasonic + DES system 17 . In order to further determine the optimal process, it is necessary to design and analyze the reaction process mechanism by establishing a reaction kinetic model 18 .…”

Section: Methodsmentioning

confidence: 99%

“…The enzyme was based on Candida Antarctica Lipase B (CALB) with immobilization on the hydrophobic carrier acrylic resin particles. The catalytic activity of immobilized lipases reached 10000 PLU/g and the optimum temperature range of 30–60°C 17 . Oleic acid, Absolute ethanol, Potassium hydroxide, Glycerol, and Choline chloride were analytical grades.…”

Section: Methodsmentioning

confidence: 99%

“…The alcohol oil ratio was 3:1 mmol/g under the ultrasonic system, and the alcohol oil ratio was 4:1 mmol/g under DES and ultrasonic + DES system. 17 In order to further determine the optimal process, it is necessary to design and analyze the reaction process mechanism by establishing a reaction kinetic model. 18 The reaction kinetics of FAEE prepared by enzymatic catalysis under three reaction systems of ultrasonic, DES, and ultrasonicassisted DES were analyzed at 313.15, 323.15, and 333.15 K, respectively.…”

Section: Reaction Kinetic Model Establishment and Design Analysismentioning

confidence: 99%

“…Of particular interest are the reaction rate, the conversion efficiency, and the recyclability of the catalyst. The system chosen for study uses as catalyst an immobilized form of the well‐known Novozym ® 435 lipase, 17 and utilizes a mixture of cooking oil waste and oleic acid as the test fatty acid stream. Excess ethanol is used as the esterification alcohol.…”

Section: Introductionmentioning

confidence: 99%

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A kinetic study of the ultrasonically assisted ethyl esterification of fatty acids using an immobilized lipase catalyst and deep eutectic solvent

Suo

Hao

Zhang

et al. 2022

Int J of Chemical Kinetics

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In this study, the esterification reaction kinetics of biodiesel (fatty acid ethyl ester) prepared from waste oil (cooking oil waste and oleic acid mixture) and ethanol at three different temperatures (313.15, 323.15, and 333.15 K) by lipase (Novozym R 435) catalyzed. It was investigated under three different reaction systems of ultrasonic (without DES), deep eutectic solvent (DES, choline chloride: glycerol mole ratio = 1:2, without ultrasonic), and ultrasonic-assisted DES. The results showed that the combination of the ultrasonic and DES had a synergistic effect on the enzymatic reaction. The activation energy of the reaction was the smallest (24.82 kJ/mol) compared to the other two processes (ultrasonic: 34.38 kJ/mol and DES: 29.43 kJ/mol), and the reaction was easier to proceed. Subsequently, the reusability of lipase and the composition of the product were studied. The results showed that the conversion rate of fatty acid ethyl ester decreased from the initial 93.33% to 80.31% when the experiment was repeated five times in the ultrasonic-assisted DES system. The ethyl ester composition of the product was mainly concentrated between C 14 and C 24 , and the proportion of ethyl oleate was the most abundant.

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“…The formed DES was placed in a vacuum drying oven at 60°C for 48 h, then cooled to room temperature. Finally, the prepared DES was sealed and stored in a dry place 17 …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Reaction Kinetic Model Establishment and Design Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A kinetic study of the ultrasonically assisted ethyl esterification of fatty acids using an immobilized lipase catalyst and deep eutectic solvent

Suo

Hao

Zhang

et al. 2022

Int J of Chemical Kinetics

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Effect of the water content and the lengthening of fatty acids chain on the iCALB‐catalyzed esterification in deep eutectic solvents

Maurente,

Callejas,

Volonterio

et al. 2023

J Americ Oil Chem Soc

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Four Deep Eutectic Solvents (DES) based on choline chloride (ChCl) and different hydrogen bond donors (1,4‐butanediol, glycerol, isosorbide, and urea) were tested as reaction medium for the immobilized Candida antarctica B (iCALB) lipase‐catalyzed esterification of fatty acids with n‐butanol. Although the strong hydrogen bonds between DES components were expected to lower their reactivity, all except urea, competed in the esterification reaction. To study the effect of acyl group donor and water, different chain length of saturated fatty acids (ranging from 6:0 to 18:0) and water content (ranging from 0, 1, 2 or 3 molar ratio of water/ChCl) were tested in the lipase‐catalyzed esterification with n‐butanol in ChCl:urea (1:2, molar ratio). When the incubation was performed in the absence of water, the shorter the fatty acid chain length, the higher the conversion. Upon water addition, a drastic increase in the esterification rate of all the fatty acids was observed and, conversely to what occurred in the anhydrous media, the longer the fatty acid chain length, the higher the conversion. Results suggest that water concentration in the DES medium possesses a multifactorial effect, as it influences the enzyme activity, the solvent viscosity, the DES structure, and the interaction between DES components, substrates, and enzymes, thus determining the efficiency and yield of the esterification reaction.

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Silica‐Mediated Incorporation of Lipase Into the Bulk of a Deep Eutectic Solvent: An Efficient Biocatalytic Phase for Esters Synthesis

Wolny,

Babiuch,

Latos

et al. 2024

ChemCatChem

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Aiming at sustainable solutions suitable for industrial‐scale catalysis catalytic phase containing lipase and Deep Eutectic Solvent (DES) was designed. To achieve this, both physical and chemical immobilizations of lipases were performed on the surface of silica and carbon materials. The catalytic activity of developed biosystem was tested in biotransformation of α‐angelica lactone into butyl levulinate at 60°C. The best results were achieved for Candida antarctica lipase B adsorbed on fumed silica suspended in choline chloride: glycerol (1:2) with the addition of 20 wt% of water. Under these conditions 99.9% conversion of α‐angelica lactone and 100% selectivity to butyl levulinate were obtained after 45 min. The biocatalytic system maintained its activity for up to five consecutive reaction cycles with full conversion of lactone to ester. The heterogenization of the enzyme allowed the biocatalyst to be integrated into the bulk of the DES, which includes essential water. This combination resulted in the formation of a stable and easy‐to‐operate catalytic phase where reagents formed a second organic phase. The integration of cost‐effective biocatalyst with process efficiency provides a greener alternative with significant potential for industrial use.

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Ultrasound-assisted enzymatic preparation of fatty acid ethyl ester in deep eutectic solvent

Cited by 18 publications

References 23 publications

A kinetic study of the ultrasonically assisted ethyl esterification of fatty acids using an immobilized lipase catalyst and deep eutectic solvent

A kinetic study of the ultrasonically assisted ethyl esterification of fatty acids using an immobilized lipase catalyst and deep eutectic solvent

Effect of the water content and the lengthening of fatty acids chain on the iCALB‐catalyzed esterification in deep eutectic solvents

Silica‐Mediated Incorporation of Lipase Into the Bulk of a Deep Eutectic Solvent: An Efficient Biocatalytic Phase for Esters Synthesis

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