Ultrasound-assisted extraction of antimicrobial compounds from Thymus daenensis and Silybum marianum: Antimicrobial activity with and without the presence of natural silver nanoparticles

Abstract

The present study is devoted to prepare a new antibacterial and antifungal agent based on in situ-synthesized silver nanoparticles at room temperature using Rosmarinus officinalis (R. officinalis) leaf extract. The Ag-NPs characterization by UV-visible, SEM, TEM and XRD revealed that the particles sizes were in the range of 10-33 nm. In this study, hydroalcoholic extracts were used with ultrasonic method. Ultrasonication has recently received attention as a novel bioprocessing tool for process intensification in many areas of downstream processing. The antimicrobial activities of T. daenensis and S. marianum extracts with and without the presence of Ag-NPs were investigated at concentrations from 12.5 to 50 mg/mL against Staphylococcus aureus (S. aureus, Gram-positive organism) and Escherichia coli (E. coli, Gram-negative organism), and fungal strains were Aspergillus oryzae (A. oryzae) and Candida albicans (C. albicans). Antimicrobial activity determined using agar disc diffusion method revealed that the activities of Ag-NPs/T. daenensis were superior to Ag-NPs/S. marianum and extracts (T. daenensis and S. marianum). The medicinal plant extract can be used to synthesize the Ag-NPs as an eco-friendly and inexpensive method in large scale. The results showed that the prepared Ag-NPs/extracts as good antibacterial and antifungal agents can be potentially applied against rapidly increasing of antibiotic resistance.

Antifungal Activity against Botryosphaeriaceae Fungi of the Hydro-Methanolic Extract of Silybum marianum Capitula Conjugated with Stevioside

“…Fernández, et al [ 63 ] found significant inhibition against Fusarium graminearum Schwabe for four flower defensins from milk thistle. Safarpoor, et al [ 64 ] reported moderate antifungal activities of ethanolic extracts of milk thistle against C. albicans and Aspergillus oryzae (Ahlb.) Cohn.…”

Silver Nanoparticles and Polyphenol Inclusion Compounds Composites for Phytophthora cinnamomi Mycelial Growth Inhibition

“…Furthermore, they can be prepared through green synthesis procedures with the aid of plant extracts [ 6 ], which act as reducing and stabilizing agents. Polyol components, polysaccharides, and water-soluble heterocycles (such as those from Stevia rebaudiana [ 7 ], Curcuma longa [ 8 ], Pongamia pinnata [ 9 ], Gliricidia sepium [ 10 ], Eucalyptus hybrida [ 11 ], or Quercus brantii [ 12 ]) have been reported to lead to a synergistic effect in the resulting phytonanocomposites [ 13 , 14 , 15 ].…”

Synthesis of CuS and ZnO/Zn(OH)2 nanoparticles and their evaluation for in vitro antibacterial and antifungal activities

“…Nanotechnology has shown tremendous potential to change society with its varied applications . Nanoparticles (NPs) of zinc, iron, gold, copper and silver are applied in various fields such as pharmaceuticals, agriculture, diagnostics, automotive industry, packaging, paints, etc .…”

“…Nanoparticles (NPs) of zinc, iron, gold, copper and silver are applied in various fields such as pharmaceuticals, agriculture, diagnostics, automotive industry, packaging, paints, etc . Their varied applications are due to their medicinal properties, and they can be synthesized using physical, chemical and biological methods . Researchers are interested in synthesizing nanoparticles and studying their size because of their unique physicochemical characteristics and applications.…”