Ultrasound‐assisted Extraction of Gymnemic Acids from <i>Gymnema sylvestre</i> Leaves and its Effect on Insulin‐producing RINm‐5 F β Cell Lines

Sheoran, Sunita; Panda, Bibhu Prasad; Admane, Prasad; Panda, Amulya K.; Wajid, Saima

doi:10.1002/pca.2540

Cited by 18 publications

(11 citation statements)

References 16 publications

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“…Taken together, the bioactive compounds present in Gymnema sylvestre could be a possible reason for better adherence and proliferation of the hDFs and HaCaT. The major bioactive compounds present in Gymnema sylvestre extracts were gymnemic acids and gymnemagenin [59,60,61]. The other phytoconstituents from Gymnema sylvestre reported were kaempferol, quercetin [62], triterpenoid saponin [63], lupeol and stigmasterol [64].…”

Section: Resultsmentioning

confidence: 99%

Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study

et al. 2019

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Electrospun fibers have emerged as promising materials in the field of biomedicine, due to their superior physical and cell supportive properties. In particular, electrospun mats are being developed for advanced wound dressing applications. Such applications require the firers to possess excellent antimicrobial properties in order to inhibit potential microbial colonization from resident and non-resident bacteria. In this study, we have developed Poly-ε-Caprolactone /gelatin hybrid composite mats loaded with natural herbal extract (Gymnema sylvestre) to prevent bacterial colonization. As-spun scaffolds exhibited good wettability and desirable mechanical properties retaining their fibrous structure after immersing them in phosphate buffered saline (pH 7.2) for up to 30 days. The initial burst release of Gymnema sylvestre prevented the colonization of bacteria as confirmed by the radial disc diffusion assay. Furthermore, the electrospun mats promoted cellular attachment, spreading and proliferation of human primary dermal fibroblasts and cultured keratinocytes, which are crucial parenchymal cell-types involved in the skin recovery process. Overall these results demonstrated the utility of Gymnema sylvestre impregnated electrospun PCL/Gelatin nanofibrous mats as an effective antimicrobial wound dressing.

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Section: Resultsmentioning

confidence: 99%

Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study

et al. 2019

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“…29 Various studies have verified that GA plays an important role in the treatment of T2DM, although the mechanism responsible for its antihypoglycemic effects has not been studied. 30,31 Based on previous studies, we chose the doses of 40 and 80 mg/kg/day to investigate the potential antidiabetic effects of GA in T2DM rats. The results showed that 6 weeks of treatment with GA alleviated the diabetic symptoms as evidenced by reduced FBG and insulin levels and HbA1c ratio.…”

Section: Discussionmentioning

confidence: 99%

Gymnemic Acid Alleviates Type 2 Diabetes Mellitus and Suppresses Endoplasmic Reticulum Stress in Vivo and in Vitro

Sun

Liu

et al. 2019

J. Agric. Food Chem.

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Gymnemic acid (GA) is an herbal ingredient that can improve glucose metabolism in patients with diabetes mellitus. In this study, we evaluated the ameliorative effects of GA on insulin resistance (IR) and identified the mechanisms in type 2 diabetes mellitus (T2DM) rats and IR HepG2 cells. GA effectively enhanced glucose uptake in IR HepG2 cells from 11.9 ± 1.09 to 14.7 ± 1.38 mmol/L and lowered fasting blood glucose (blood glucose levels in groups treated with GA at 40 and 80 mg/kg/day were reduced by 15.2% and 26.7%, respectively) and oral glucose tolerance. Both in vivo and in vitro, GA downregulated the expression of endoplasmic reticulum (ER) stress indicator proteins such as ORP150, p-c-Jun, p-PERK, and p-eIF2α. In addition, the improvement of ER stress regulated the insulin signal transduction proteins, reducing p-IRS-1(ser) levels and increasing p-IRS-1(tyr) in GA-treated T2DM rats and IR HepG2 cells. In summary, the mechanism underlying the hypoglycemic effects of GA may be associated with alleviation of ER stress and facilitation of insulin signal transduction in T2DM rats and IR HepG2 cells.

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“…From previous findings, it is reported that fermentation of G. sylvestre leaves showed better activity as compared to natural extract . Gymnemic acid is the major bioactive constituent, and it easily converted into gymnemagenin.…”

Section: Resultsmentioning

confidence: 96%

“…The heated solution cooled and extracted with chloroform. The chloroform extract was concentrated to get gymnemagenin . While the methanolic solution of extract and fermented materials were also used for HPTLC analysis.…”

Section: Methodsmentioning

confidence: 99%

Metabolic differentiation and quantification of gymnemic acid in Gymnema sylvestre (Retz.) R.Br. ex Sm. leaf extract and its fermented products

Khan

Sheoran

Khan

et al. 2020

Phytochemical Analysis

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Introduction Gymnemagenin is the bioactive metabolite found in Gymnema sylvestre leaves and possesses different therapeutic potential. Due to its lower abundance and higher market potential, gymnemagenin was obtained from chemical conversion and bacterial biotransformation. Objective To obtain the probiotic‐based fermentative conversion of gymnemic acid‐enriched G. sylvestre leaf extract to gymnemagenin‐containing nutraceuticals and its metabolites based chromatographic comparison. Material and Methods Gymnema sylvestre leaves were extracted through soxhalation, and the extract was prepared and characterised. Gymnemic acid was fermented, separately, by Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, and by their mix co‐culture. The fermented materials were analysed for their gymnemagenin content, antioxidant potential, antidiabetic potential, and metabolomics analysis. Results Extraction yielded about 35% w/w of raw plant material, and 8.5% was found to be as total saponin content. Extract at higher concentration (≥ 5%, w/v) significantly altered the growth behaviour of probiotics. High‐performance thin‐layer chromatography (HPTLC) based quantification of gymnemagenin revealed that a maximum increase of 95.5% gymnemagenin was found in extract incubated with B. bifidum followed by mix co‐culture containing (B. bifidum, L. casei, and L. rhamnosus), L. casei, and L. rhamnosus. However, liquid chromatography mass spectrometry (LC‐MS) analysis resulted in the identification of a total of 56 metabolites. Conclusion Chromatographically profiled, and probiotic‐based fermented G. sylvestre leaves can be used as a potent nutraceutical for diabetes and other metabolic disorders.

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Ultrasound‐assisted Extraction of Gymnemic Acids from Gymnema sylvestre Leaves and its Effect on Insulin‐producing RINm‐5 F β Cell Lines

Cited by 18 publications

References 16 publications

Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study

Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study

Gymnemic Acid Alleviates Type 2 Diabetes Mellitus and Suppresses Endoplasmic Reticulum Stress in Vivo and in Vitro

Metabolic differentiation and quantification of gymnemic acid in Gymnema sylvestre (Retz.) R.Br. ex Sm. leaf extract and its fermented products

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