High-frequency ultrasound biomicroscopy (UBM) (Paradigm Medical Industries, Salt Lake City, Utah) provides high-resolution in vivo imaging of the anterior segment in a noninvasive fashion. In addition to the tissues easily seen using conventional methods (ie, slit lamp), such as the cornea, iris, and sclera, structures including the ciliary body and zonules, previously hidden from clinical observation, can be imaged and their morphology assessed. Pathophysiologic changes involving anterior segment architecture can be evaluated qualitatively and quantitatively. This article discusses the role of UBM in imaging of the anterior segment of the eye from the qualitative and quantitative analysis point of view.
Equipment and techniqueThe technology for UBM, originally developed by Pavlin, Sherar, and Foster, is based on 50-to 100-MHz transducers incorporated into a B-mode clinical scanner [1][2][3]. Higher frequency transducers provide finer resolution of more superficial structures, whereas lower frequency transducers provide greater depth of penetration with less resolution. The commercially available units operate at 50 MHz and provide lateral and axial physical resolutions of approximately 50 μm and 25 μm, respectively. Tissue penetration is approximately 4 to 5 mm. The scanner produces a 5 × 5 mm field with 256 vertical image lines (or A-scans) at a scan rate of 8 frames per second.Each A-scan is mapped into oversampled 1024 points, with 256 gray-scale levels representing the logged amplitude of reflection, and then the number of points is downsized to 432 pixels to fit on the UBM monitor. The real-time image is displayed on a video monitor and can be recorded on videotape for later analysis. Room illumination, fixation, and accommodative effort affect anterior segment anatomy and should be held constant, particularly when quantitative information is being gathered.The image acquisition technique has been described elsewhere and is similar to traditional immersion B-scan ultrasonography [3][4][5]. In the Paradigm Instruments UBM, the probe is suspended from a gantry arm to minimize motion artifacts, and lateral distortion is minimized by a linear scan format. In the OTI (Ophthalmic Technologies, Toronto, Canada) device, the probe is small and light enough not to require a suspension arm, and a sector scanning method is used. Scanning is performed with the patient in the supine position. A plastic eyecup of the appropriate size is inserted between the lids, holding methylcellulose or normal saline coupling medium. To maximize the detection of the reflected signal, the transducer should be oriented so that the scanning ultrasound beam strikes the target surface perpendicularly. In the normal eye, the cornea, anterior chamber, posterior chamber, iris, ciliary body, and anterior lens surface can be recognized easily (Fig. 1). The scleral spur is the only constant landmark allowing one to interpret UBM images in terms of the morphologic status of the anterior chamber angle and is the key for analyzing angle pathology. ...