Ultrasound-guided drug delivery in cancer

Chowdhury, Sayan Mullick; Lee, Taehwa; Willmann, Jürgen K.

doi:10.14366/usg.17021

Cited by 164 publications

(101 citation statements)

References 95 publications

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“…One of the most common commercial formulation to achieve this goal is mainly represented by SonoVue © (Bracco, Milan, Italy) [ 2 ], a solution of sulfur hexafluoride microbubbles (diameter 1-6 μm) encapsulated by a phospholipid membrane, which prevents its fast dissolution in blood. Recently, therapeutic applications of microbubbles have been rising, such as: i) drug delivery [ 3 ]; ii) gene delivery [ 4 ]; iii) antibodies targeting [ 5 ]. This capability is enhanced in case of a combination of microbubbles with sonoporation [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Sonoporation by microbubbles as gene therapy approach against liver cancer

et al. 2018

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IntroductionAn innovative method, known as sonoporation, was used to induce the expression of silenced genes, such as (but not restricted to) TRAIL and p53, in liver cancer cells (HepG2). The principal aim of the present study was the re-activation of silenced apoptotic pathways in liver cancer models, by using diagnostic synovial microbubble as plasmid gene delivery tools in combination with epigenetic treatments.Material and methodsHepG2 cells were used as a liver cancer model. Microbubbles (Sonovue®) were chosen as gene deliver system in combination with the sonoporation approach. Plasmid pEGFP-TRAIL and pEGFP-p53 were selected and propagated in Escherichia coli grown in LB broth, in order to obtain the necessary amount.ResultsSonoporation was induced by using transducer (Sonitron 2000) and, among the several conditions tested, 3 MHz, 51% Duty Cycle, and 5 W/cm2, 30 s resulted as the best parameters. Data collected showed a dose dependent effect in terms of output energy. A transfection efficacy of 30 – 50% was achieved and recombinant gene expression induced apoptotic effects. In order to increase efficacy, we used the histone deacetylase inhibitor (HDACi, entinostat) MS-275, able to activate TRAIL and thus inducing a stronger pro-apoptotic effect in combination with TRAIL-gene re-expression.ConclusionFor the first time, it was shown the possibility to induce the exogenous expression of the pro-apoptotic gene TRAIL and p53 in a liver cancer HepG2 cells via a sonoporation procedure. The epigenetic treatment using HDACi was able to increase the pro-apoptotic effects of the gene therapy.

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Section: Introductionmentioning

confidence: 99%

Sonoporation by microbubbles as gene therapy approach against liver cancer

et al. 2018

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“…Microbubbles help in decreasing the side effects of chemotherapy by encapsulating the drug within the shell and releasing the drug on the specific site thereby minimizing the drug contact with the normal tissues [ 6 ]. Recent growth in the ultrasound and microbubble-mediated drug delivery technology can improve targeted drug delivery thereby reducing drug dose and adverse effects [ 7 ]. This is carried out through a mechanism called sonoporation which is the formation of openings in the blood vessels induced by oscillations triggered by ultrasound and the destruction of microbubbles [ 7 ].…”

Section: Reviewmentioning

confidence: 99%

“…Recent growth in the ultrasound and microbubble-mediated drug delivery technology can improve targeted drug delivery thereby reducing drug dose and adverse effects [ 7 ]. This is carried out through a mechanism called sonoporation which is the formation of openings in the blood vessels induced by oscillations triggered by ultrasound and the destruction of microbubbles [ 7 ].…”

Section: Reviewmentioning

confidence: 99%

Ultrasound-guided Microbubble in the Treatment of Cancer: A Mini Narrative Review

Rajendran¹

2018

Cureus

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Microbubble is an emerging modality in the field of Medicine for treatment and imaging. Ultrasound-guided microbubble is an effective diagnosing and treatment technique as it can reduce the systemic toxicity of chemotherapeutic drugs. It is also used in targeted gene delivery in gene therapy. The objective of the review article is to formulate a narrative review on the emerging importance of microbubbles in the diagnosis and treatment of cancer and its future in cancer management. The article focuses on the effectiveness of ultrasound-targeted microbubble in the treatment of malignancy.

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“…Previous studies have focused on using ultrasound-triggered microbubble destruction (UTMD) for the treatment of cancer (13,14). Lipid microbubbles, which are widely used as acoustic contrast agents, are also considered to function as good carriers for drug delivery (15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Effect of docetaxel‑loaded lipid microbubble in combination with ultrasound‑triggered microbubble destruction on the growth of a gastric cancer cell line

Lai

Zhu

et al. 2019

Oncol Lett

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Although gastric cancer therapy has been improved, more efficient treatment strategies still need to be developed. In the present study, a docetaxel (DOC)-loaded lipid microbubble (DLLD) was prepared and the effect of DLLD combined with ultrasound-triggered microbubble destruction (UTMD) on the growth of a gastric cancer cell line was investigated. The following four groups were included in the present study: Control, DOC, DLLD and DLLD plus UTMD. The determined entrapment efficiency of DLLD is 76±3.5%. The present study demonstrated that treatment with DLLD plus UTMD could significantly inhibit the growth of the cultured gastric cancer cell line BGC-823 via arresting the cell cycle in G 2 /M phase, inhibiting cell DNA synthesis, promoting cell apoptosis and disrupting mitochondrial membrane potential, as compared with treatment with DOC or DLLD alone. Furthermore, the expression of p53, p21 and Bax were identified to be significantly upregulated, while that of Bcl-2 was significantly downregulated in the DLLD plus UTMD group. Therefore, treatment with DLLD plus UTMD was more efficient in inhibiting cell proliferation and inducing cell apoptosis in the gastric cancer cell line, when compared with treatment with DOC or DLLD alone, suggesting that DLLD plus UTMD could serve as a promising strategy for the treatment of gastric cancer.

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Ultrasound-guided drug delivery in cancer

Cited by 164 publications

References 95 publications

Sonoporation by microbubbles as gene therapy approach against liver cancer

Sonoporation by microbubbles as gene therapy approach against liver cancer

Ultrasound-guided Microbubble in the Treatment of Cancer: A Mini Narrative Review

Effect of docetaxel‑loaded lipid microbubble in combination with ultrasound‑triggered microbubble destruction on the growth of a gastric cancer cell line

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