Ultrasound influence on coleoptile length at Poaceae seedlings as valuable criteria in prebreeding and breeding processes

Abstract

The study includes research on the effect of ultrasound on the ability of seed germination and coleoptile development of cereal landraces (fam. Poaceae): oat (Avena sativa L.) brodski, rye (Secale cereale L.) rakotinska, Triticale svetinikolsko and soft wheat (Triticum aestivum L.) govrlevska. The experiment with ultrasound was carried out at frequency of 30-40 kHz on the thermostatic ultrasonic bath. Seeds were sonicated at a constant temperature (25ºC) for 15 min. Ultrasound effect is reflected in the length of the coleoptyl and mesocotyl, although in a good deal is genetically predetermined. Coleoptile length was the longest in Triticale (3.3-5.5 cm) and approximately the same lengths are evident in rye (3.0-6.1 cm). Concerning the mesocotyl the longest one (5.525 cm ± 0.697) is recorded at rye, even significantly longer than control of oat and Triticale. Longer coleoptile of sonicated seeds indicates faster seedling development, good water supply and rapid development and emergence of first leaves. Correlation coefficient showed very high (0.821) and high R2 (67,472%) dependence among variables both, in length of coleoptiles (as dependent variable) and mesocotyl (as independent) with low standard error (0.225). With the simple use of ultrasound the germination period could be shorten, water supply more efficient, the sowing periods will be shorten and good yields even under conditions of climate change with increased temperatures could be achieved

Evaluation of techniques to break seed dormancy in Redroot pigweed (Amaranthus retroflexus)

“…Our data show that pretreating seeds with ultrasound effectively promoted germination percentage and rate (Figure 1 , Table 2 ). This finding is consistent with previous studies in other species that have utilized ultrasound to promote seed germination (Babaei‐Ghaghelestany et al., 2020 ; Ding et al., 2018 ; Kratovalieva et al., 2012 ; Miano et al., 2015 ; Rifna et al., 2019 ). For example, Ding et al.…”

“…Our data show that pretreating seeds with ultrasound effectively promoted germination percentage and rate (Figure 1, Table 2). This finding is consistent with previous studies in other species that have utilized ultrasound to promote seed germination (Babaei-Ghaghelestany et al, 2020;Ding et al, 2018;Kratovalieva et al, 2012;Miano et al, 2015;Rifna et al, 2019). For example, Ding Ultrasound treatments are believed to induce changes in the seed coat, facilitating influx or efflux of water and mineral elements into cells (Miano et al, 2015;Qin et al, 2012) and/or to increase the activity of alpha-amylase enzyme and therefore the rate of starch metabolism (Yaldagard et al, 2007(Yaldagard et al, , 2008.…”

Ultrasonic seed treatment and Cu application modulate photosynthesis, grain quality, and Cu concentrations in aromatic rice

“…However, under similar Cu application treatment, ultrasonic seed treatment showed lower PN at flowering stage (Table 1). Ultrasonic seed treatment may cause physiobiochemical changes in seeds, e.g., elongation in coleoptiles and mesocotyl, thus accelerated seminal roots growth which may resulted in increased absorption of water and other mineral nutrients that ultimately led to enhanced growth and development of crop plants (Kratovalieva et al 2012).…”

Impact of low-frequency ultrasound technology on physical, chemical and technological properties of cereals and pseudocereals

“…Similarly, Hassan et al [61] observed that 5 min LFUS treatment greatly improved the number of germinated sorghum grains from 78% (control) to 94%; a longer process decreased the germinated seeds, therefore they inferred that at high amplitude the treatment damaged cells and/or enzymes. Kratovalieva et al [62] studied LFUS application to different cereals and noticed significant effects on coleoptile and mesocotyl elongation. Oat and rye caryopses were very sensitive to LFUS stimulation (+119% and +65% growth, respectively), while triticale and wheat showed smaller increases.…”