Ultrasound-Mediated EGF-Coated-Microbubble Cavitation in Dressings for Wound-Healing Applications

Liao, Ai‐Ho; Hung, Chi-Ray; Chen, Hang-Kang; Chiang, Chien-Ping

doi:10.1038/s41598-018-26702-z

Cited by 27 publications

(16 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCD. Our previous studies have suggested that USMB disruption (i.e., inertial cavitation) is required for effective transdermal drug delivery (42)(43)(44). Recent studies have demonstrated that the acoustic-emission signals acquired in PCD could be used to quantify the subharmonic-frequency component and inertial cavitation dose, which represent the index of stable cavitation and inertial cavitation, respectively (45,46).…”

Section: Methodsmentioning

confidence: 99%

Ultrasound-induced microbubble cavitation via a transcanal or transcranial approach facilitates inner ear drug delivery

Liao¹,

Wang²,

Weng³

et al. 2020

JCI Insight

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Ultrasound-induced microbubble cavitation via a transcanal or transcranial approach facilitates inner ear drug delivery

Liao¹,

Wang²,

Weng³

et al. 2020

JCI Insight

Self Cite

View full text Add to dashboard Cite

“…12,13 Ultrasound microbubbles (USMBs) are a new type of DDS for the treatment of bacterial infection. [14][15][16][17] A number of publications have indicated that ultrasound with cavitation can enhance the inhibitory effects of antimicrobial agents on bacterial biofilms, which can be amplified by microbubbles. 18,19 As a result, USMBs can promote the bacterial uptake of antimicrobials and improve the antibacterial efficacy of drugs.…”

Section: Introductionmentioning

confidence: 99%

<p>Synergistic antibacterial effect of ultrasound microbubbles combined with chitosan-modified polymyxin B-loaded liposomes on biofilm-producing <em>Acinetobacter baumannii</em></p>

Fu¹,

Zhang²,

Yang³

et al. 2019

IJN

View full text Add to dashboard Cite

Purpose: Resistant strains of Acinetobacter baumannii (AB) that can form biofilms are resistant to polymyxin. Therefore, effective and safe polymyxin preparations against biofilm-producing AB are urgently needed. This study aims to prepare chitosan-modified polymyxin B-loaded liposomes (CLPs) and ultrasound microbubbles (USMBs) and then explore the synergistic antibacterial effects of USMBs combined with CLPs in vitro. Methods: CLPs were prepared using a modified injection method, and microbubbles were prepared using a simple mechanical vibration method. Minimal biofilm inhibitory concentration (MBIC) of CLPs against resistant biofilm-producing AB was determined. Antibacterial activities of CLPs with or without USMBs were analyzed by crystal violet staining and resazurin assays to evaluate biofilm mass and viable counts, respectively. Then, the anti-biofilm effects of CLPs with or without USMBs on biofilm-producing AB were confirmed via scanning electron microscopy (SEM) analysis. Results: We prepared CLPs that were 225.17±17.85 nm in size and carried positive charges of 12.64±1.44 mV. These CLPs, with higher encapsulation efficiency and drug loading, could exhibit a sustained release effect. We prepared microbubbles that were 2.391±0.052 µm in size and carried negative charges of −4.32±0.43 mV. The MBICs of the CLPs on the biofilmproducing AB was 8±2 µg/mL, while that of polymyxin B was 32±2 µg/mL. USMBs in combination with 2 µg/mL of polymyxin B could completely eliminate the biofilm-producing AB and achieve the maximum antimicrobial effects (P.0.05 vs sterile blank control). SEM imaging revealed some scattered bacteria without a biofilm structure in the USMB combined with the CLP group, confirming that this combination has the greatest anti-biofilm effects. Conclusion: In this research, we successfully prepared USMBs and CLPs that have a more significant antibacterial effect on biofilm-forming AB than polymyxin B alone. Experiments in vitro indicate that the synergistic antibacterial effect of combining USMBs with CLPs containing as little as 2 µg/mL of polymyxin B is sufficient to almost eliminate drug-resistant biofilm-producing AB.

show abstract

“…Previous studies have suggested that US-mediated MB disruption (i.e., inertial cavitation) is required for effective TDD [8,16,17]. The present study measured the efficacy of MB disruption when using different types of lasers under different conditions.…”

Section: Methodsmentioning

confidence: 90%

Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug Delivery

et al. 2018

Self Cite

View full text Add to dashboard Cite

The optodynamic process of laser-induced microbubble (MB) cavitation in liquids is utilized in various medical applications. However, how incident laser radiation interacts with MBs as an ultrasound contrast agent is rarely estimated when the liquid already contains stable MBs. The present study investigated the efficacy of the laser-mediated cavitation of albumin-shelled MBs in enhancing transdermal drug delivery. Different types and conditions of laser-mediated inertial cavitation of MBs were first evaluated. A CO2 fractional pulsed laser was selected for combining with MBs in the in vitro and in vivo experiments. The in vitro skin penetration by β-arbutin after 2 h was 2 times greater in the group combining a laser with MBs than in the control group. In small-animal experiments, the whitening effect on the skin of C57BL/6J mice in the group combining a laser with MBs on the skin plus penetrating β-arbutin increased (significantly) by 48.0% at day 11 and 50.0% at day 14, and then tended to stabilize for the remainder of the 20-day experimental period. The present results indicate that combining a CO2 laser with albumin-shelled MBs can increase skin permeability so as to enhance the delivery of β-arbutin to inhibit melanogenesis in mice without damaging the skin.

show abstract

Ultrasound-Mediated EGF-Coated-Microbubble Cavitation in Dressings for Wound-Healing Applications

Cited by 27 publications

References 41 publications

Ultrasound-induced microbubble cavitation via a transcanal or transcranial approach facilitates inner ear drug delivery

Ultrasound-induced microbubble cavitation via a transcanal or transcranial approach facilitates inner ear drug delivery

<p>Synergistic antibacterial effect of ultrasound microbubbles combined with chitosan-modified polymyxin B-loaded liposomes on biofilm-producing <em>Acinetobacter baumannii</em></p>

Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug Delivery

Contact Info

Product

Resources

About