Ultrasound microbubble-carried PNA targeting to c-myc mRNA inhibits the proliferation of rabbit iliac arterious smooth muscle cells and intimal hyperplasia

He, Xijing; Wu, Di; Ji, Jun; Wen-ping, Ling; Chen, Xiaoling; Chen, Yue-Xuan

doi:10.3109/10717544.2015.1014947

Cited by 11 publications

(7 citation statements)

References 27 publications

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“…Agents for microbubble ultrasound contrast emerge as novel vectors for gene transfection, as they showed high safety, stability, and efficiency [ 10 ]. Microbubbles ‘break’ and release target gene under the energy from ultrasound radiation, which can increase cell permeability and create a reversible sound hole that promotes the infiltration of target genes into the nucleus and therefore increase gene transfection and expression efficiency [ 11 , 12 ]. In clinical practice, applying microbubble ultrasound agent in gene therapy has attracted much attention due to its high efficiency of transfection and low side effects [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 inhibits breast cancer cells proliferation and metastasis, but promotes apoptosis

Luo

et al. 2020

Bioscience Reports

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This study compared the effects of ultrasonic irradiation and SonoVue microbubbles (US) or Lipofectamine 3000 on the transfection of small interfering RNA for PRR11 (siPRR11) and PRR11 overexpression plasmid into breast cancer cells. SiPRR11 and PRR11 overexpression plasmid were transfected into breast cancer MCF7 cells mediated by US and Lipofectamine 3000. PRR11 expressions in breast cancer and normal tissues were determined using Gene Expression Profiling Interactive Analysis (GEPIA). The viability, proliferation, migration, invasion and apoptosis of breast cancer cells were respectively measured by MTT assay, clone formation assay, scratch wound-healing assay, Transwell assay and flow cytometry. PRR11 and epithelial-to-mesenchymal transition (EMT)-related and apoptosis-related (B-cell lymphoma 2, Bcl-2; Bcl-2 associated protein X, Bax) proteins expressions were by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as appropriate. As ultrasonic intensity increased, the viability of MCF7 cells was decreased. Results from GEPIA suggested that PRR11 was up-regulated in breast cancer. Silencing PRR11 mediated by US showed a higher efficiency than by Lipofectamine 3000. SiPRR11 transfected by Lipofectamine 3000 suppressed cells growth and metastasis, while promoted cell apoptosis. Moreover, E-cadherin and Bax expressions were low but N-cadherin, Snail and Bcl-2 expressions were high. However, overexpressed PRR11 caused the opposite effects. More importantly, transfection of siPRR11 and PRR11 overexpression plasmid using US had a higher efficacy than using Lipofectamine 3000. US transfection of PRR11 siRNA showed better effects on inhibiting breast cancer progression. The current findings contribute to a novel treatment for breast cancer.

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Section: Introductionmentioning

confidence: 99%

Ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 inhibits breast cancer cells proliferation and metastasis, but promotes apoptosis

Luo

et al. 2020

Bioscience Reports

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“…The size of microbubbles (about 1-10 mm) enables them to circulate with red blood cells (Jayaweera et al, 1994;Sirsi and Borden, 2012;Mulvana et al, 2017). Microbubbles, as proven ultrasound-responsive materials, have been applied in drug delivery in clinical trials (Table 1) (Hynynen et al, 2001;Dimcevski et al, 2016;He et al, 2016). These clinical trials confirmed the controllability of delivering the cargo like drugs and gene materials with ultrasonic switch and visualization of treatment.…”

Section: Introductionmentioning

confidence: 83%

Ultrasound-Responsive Materials for Drug/Gene Delivery

et al. 2020

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Ultrasound is one of the most commonly used methods in the diagnosis and therapy of diseases due to its safety, deep penetration into tissue, and non-invasive nature. In the drug/gene delivery systems, ultrasound shows many advantages in terms of site-specific delivery and spatial release control of drugs/genes and attracts increasing attention. Microbubbles are the most well-known ultrasound-responsive delivery materials. Recently, nanobubbles, droplets, micelles, and nanoliposomes have been developed as novel carriers in this field. Herein, we review advances of novel ultrasound-responsive materials (nanobubbles, droplets, micelles and nanoliposomes) and discuss the challenges of ultrasound-responsive materials in delivery systems to boost the development of ultrasound-responsive materials as delivery carriers.

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“…Previous studies have shown that increased expression of c-MYC protein is closely associated with certain cardiovascular diseases, such as atherosclerosis, hypertension and restenosis 50 . c-MYC belongs to the basic helix-loop-helix leucine zipper (bHLHZip) family of transcription factors and can induce the continuous proliferation of VSMCs 51 - 53 . In this study, we demonstrated that inhibition of c-MYC expression was a key point for the anti-proliferative effect of celastrol in VSMCs.…”

Section: Discussionmentioning

confidence: 99%

Celastrol ameliorates vascular neointimal hyperplasia through Wnt5a-involved autophagy

Shi¹,

Liu²,

Deng³

et al. 2021

Int. J. Biol. Sci.

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Neointimal hyperplasia caused by the excessive proliferation of vascular smooth muscle cells (VSMCs) is the pathological basis of restenosis. However, there are few effective strategies to prevent restenosis. Celastrol, a pentacyclic triterpene, has been recently documented to be beneficial to certain cardiovascular diseases. Based on its significant effect on autophagy, we proposed that celastrol could attenuate restenosis through enhancing autophagy of VSMCs. In the present study, we found that celastrol effectively inhibited the intimal hyperplasia and hyperproliferation of VSMCs by inducing autophagy. It was revealed that autophagy promoted by celastrol could induce the lysosomal degradation of c-MYC, which might be a possible mechanism contributing to the reduction of VSMCs proliferation. The Wnt5a/PKC/mTOR signaling pathway was found to be an underlying mechanism for celastrol to induce autophagy and inhibit the VSMCs proliferation. These observations indicate that celastrol may be a novel drug with a great potential to prevent restenosis.

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Ultrasound microbubble-carried PNA targeting to c-myc mRNA inhibits the proliferation of rabbit iliac arterious smooth muscle cells and intimal hyperplasia

Cited by 11 publications

References 27 publications

Ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 inhibits breast cancer cells proliferation and metastasis, but promotes apoptosis

Ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 inhibits breast cancer cells proliferation and metastasis, but promotes apoptosis

Ultrasound-Responsive Materials for Drug/Gene Delivery

Celastrol ameliorates vascular neointimal hyperplasia through Wnt5a-involved autophagy

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