Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles

Liu, Yuan; Feshitan, Jameel A.; Wei, Ming; Borden, Mark A.; Yuan, Baohong

doi:10.1117/1.jbo.20.3.036012

Cited by 6 publications

(8 citation statements)

References 28 publications

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“…UMF contrast agent using microbubble as a carrier and a mediator. (A) single fluorophore-labeled microbubble (Liu et al, 2014) (B) donor- and acceptor-labeled microbubble (Liu et al, 2015). Reproduced with permission.…”

Section: Ultrasound Modulated Fluorescence (Umf)mentioning

confidence: 99%

Newly-Engineered Materials for Bio-Imaging Technology: A Focus on the Hybrid System of Ultrasound and Fluorescence

Pei

Wei

2019

Front. Bioeng. Biotechnol.

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As an emerging technique, ultrasound-modulated fluorescence (UMF), or ultrasound switchable fluorescence (USF) bioimaging has shown promising features to produce deep-tissue and high-resolution fluorescence imaging for biomedical research and health diagnosis. The success of UMF or USF heavily relies on the design of their contrast agents (CAs). We herein surveyed recent advances in the development of such unique CAs, including configuration, mechanism, stability, sensitivity, and selectivity. Meanwhile, UMF or USF instrumentation has emerged as developmental breakthrough technologies to existing bio-imaging techniques. The best performance of UMF or USF bio-imaging requires an interactive response between CAs and the instrument. In this review, the description of UMF or USF instrumentation are also included for clarification and better understanding. Finally, the UMF and USF's performance in bioimaging is evaluated based on signal-to-noise ratio, resolution, imaging depth and speed, using photoacoustic imaging (PAI) as a standard, a well-developed technique of hybrid bio-imaging. Unlike PAI, UMF or USF is still in its early stage. Although results demonstrated a proof-of-concept landmark being reached, significant efforts are needed to improve the performance of UMF or USF.

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Section: Ultrasound Modulated Fluorescence (Umf)mentioning

confidence: 99%

Newly-Engineered Materials for Bio-Imaging Technology: A Focus on the Hybrid System of Ultrasound and Fluorescence

Pei

Wei

2019

Front. Bioeng. Biotechnol.

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“…Biomedical imaging contributes enormously to the diagnosis of disease by providing valuable information such as disease type, location, severity, and mechanism. Fluorescence imaging is one of the major noninvasive imaging strategies, attracting immense interests with medical imaging applications . One of the advantages of fluorescence imaging is that molecular targeting and dynamic development track in real time with carefully engineered contrast agents .…”

Section: Introductionmentioning

confidence: 99%

A Biocompatible and Near‐Infrared Liposome for In Vivo Ultrasound‐Switchable Fluorescence Imaging

Liu

Yao

Cai

et al. 2020

Adv Healthcare Materials

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Fluorescence imaging is a remarkable tool for molecular targeting and multicolor imaging, but it suffers from low resolution in centimeter‐deep tissues. The recently developed ultrasound‐switchable fluorescence (USF) imaging has overcome this challenge and achieved in vivo imaging in a mouse with help from the indocyanine green (ICG) dye encapsulated poly(N‐isopropylacrylamide) (ICG‐PNIPAM) contrast agent. However, the ICG‐PNIPAM has shortcomings, such as concerns about cytotoxicity and blueshifted excitation and emission spectra. This study introduces a newly developed ICG‐encapsulated liposome to broaden the contrast agent selection for USF imaging and resolve the issues mentioned above. The emission peak of the ICG‐liposome is 836 nm with excellent biostability and USF imaging capability. Furthermore, the cell viability test verifies the low cytotoxicity feature. Eventually, both ex vivo and in vivo USF imaging are successfully achieved and 3D USF images are acquired. The ex vivo result confirms that the ICG‐liposome maintains the thermoresponsive characteristic at the right lobe of the liver and is able to conduct the USF imaging. The further in vivo USF imaging demonstrates that although the whole liver emitted fluorescence, only the right lobe of the liver contains the working ICG‐liposome.

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“…The modulated fluorescent signal has been enhanced through the use of fluorophore labeled microbubbles that act as contrast agents which cyclically emit or quench fluorescent emission via US induced oscillation of the microbubbles . To date microbubbles labeled with donor–quencher pairs have been the most commonly used approach …”

Section: Introductionmentioning

confidence: 99%

Nanoscale Ultrasound‐Switchable FRET‐Based Liposomes for Near‐Infrared Fluorescence Imaging in Optically Turbid Media

Zhang

Morgan

Mather

2017

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A new approach for fluorescence imaging in optically turbid media centered on the use of nanoscale ultrasound-switchable FRET-based liposome contrast agents is reported. Liposomes containing lipophilic carbocyanine dyes as FRET pairs with emission wavelengths located in the near-infrared window are prepared. The efficacy of FRET and self-quenching for liposomes with a range of fluorophore concentrations is first calculated from measurement of the liposome emission spectra. Exposure of the liposomes to ultrasound results in changes in the detected fluorescent signal, the nature of which depends on the fluorophores used, detection wavelength, and the fluorophore concentration. Line scanning of a tube containing the contrast agents with 1 mm inner diameter buried at a depth of 1 cm in a heavily scattering tissue phantom demonstrates an improvement in image spatial resolution by a factor of 6.3 as compared with images obtained in the absence of ultrasound. Improvements are also seen in image contrast with the highest obtained being 9% for a liposome system containing FRET pairs. Overall the results obtained provide evidence of the potential the nanoscale ultrasound-switchable FRET-based liposomes studied here have for in vivo fluorescence imaging.

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Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles

Cited by 6 publications

References 28 publications

Newly-Engineered Materials for Bio-Imaging Technology: A Focus on the Hybrid System of Ultrasound and Fluorescence

Newly-Engineered Materials for Bio-Imaging Technology: A Focus on the Hybrid System of Ultrasound and Fluorescence

A Biocompatible and Near‐Infrared Liposome for In Vivo Ultrasound‐Switchable Fluorescence Imaging

Nanoscale Ultrasound‐Switchable FRET‐Based Liposomes for Near‐Infrared Fluorescence Imaging in Optically Turbid Media

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