Ultrasound-negative pressure cavitation extraction of phenolic compounds from blueberry leaves and evaluation of its DPPH radical scavenging activity

Wang, Tong; Guo, Na; Wang, Sui-Xin; Kou, Ping; Zhao, Chunjian; Fu, Yujie

doi:10.1016/j.fbp.2018.01.003

Cited by 56 publications

(35 citation statements)

References 42 publications

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“…They suggested that these low levels could be due to the ability of water to extract more nonphenolic substances such as carbohydrates, or to the formation of complexes involving phenolic compounds which may decrease their solubility in water. Wang et al [ 24 ] found no increase in TPC and TFC with extraction time beyond 15 min when extracting blueberry leaves using ultrasound-negative pressure cavitation extraction. With USAE of dried chokeberries in 50% ethanol, Ćujić et al [ 25 ] reported no difference between the TPC obtained after 30 and 60 min.…”

Section: Resultsmentioning

confidence: 99%

“…This suggests that excessive dilution of the plant material may not lead to further enhancement of TAC under the conditions investigated. Excess of solvent, without beneficial increase in phenolic contents, should also be avoided as it leads to solvent wastage and unwarranted increase of operating costs [ 24 ]. In the present study with USAE and blueberry pomace, a ratio of 1/15 in 50% ethanol was found to be a suitable compromise and was used in subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

“…At all three ethanol concentrations, TPC values in their work were similar to the value we obtained using 50% ethanol. Wang et al [ 24 ] suggested that the range of 60–70% ethanol is the most suitable for ultrasound-negative pressure cavitation extraction of polyphenols from blueberry leaves. They found that TPC and TFC were increased when increasing ethanol concentration from 40% to 70%, while further increase of ethanol concentration up to 90% decreased the extraction yield.…”

Section: Resultsmentioning

confidence: 99%

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Influence of Extraction Conditions on Ultrasound-Assisted Recovery of Bioactive Phenolics from Blueberry Pomace and Their Antioxidant Activity

et al. 2018

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The increase in diet-related chronic diseases has prompted the search for health-promoting compounds and methods to ensure their quality. Blueberry pomace is a rich yet underutilized source of bioactive polyphenols. For these high-value bioactive molecules, ultrasound-assisted extraction (USAE) is an attractive and green alternative to conventional extraction techniques for improving purity and yields. This study aimed to assess the impact of USAE parameters (sonication time, solvent composition, solid/liquid ratio, pH and temperature) on the recovery of phenolic compounds from blueberry pomace and antioxidant activity of the extracts. Total phenolic, flavonoid and anthocyanin contents (TPC, TFC and TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity were analysed. USAE in 50% ethanol/water was the most efficient, yielding the highest TPC (22.33 mg/g dry matter (DM)), TFC (19.41 mg/g DM), TAC (31.32 mg/g DM) and DPPH radical scavenging activity (41.79 mg Trolox/g DM). USAE in water showed the lowest values even at low (1/40) solid/liquid ratio (7.85 mg/g DM, 3.49 mg/g DM, and 18.96 mg/g DM for TPC, TFC and TAC, respectively). Decreasing the solid/liquid ratio in water or 50% ethanol significantly increased TPC, TFC, TAC and DPPH radical scavenging. With ethanol, increasing the temperature in the range 20–40 °C decreased TPC but increased TFC and DPPH radical scavenging activity. Anthocyanin profiles of water and ethanolic extracts were qualitatively similar, consisting of malvidin, delphinidin, petunidin and cyanidin. These findings indicate that USAE is a method of choice for extracting high-value bioactive phenolics from blueberry pomace. Selective enrichment of different phenolic fractions is possible under select extraction conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Influence of Extraction Conditions on Ultrasound-Assisted Recovery of Bioactive Phenolics from Blueberry Pomace and Their Antioxidant Activity

et al. 2018

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“…With UAE of dried chokeberries in 50% (v/v) ethanol, Ćujić et al [2016] reported that the total anthocyanin contents were signifi cantly greater after longer period of extraction. Wang et al [2018] found the highest yields of TPC and TFC with extraction time of 15 min when extracting blueberry leaves using ultrasound-negative pressure cavitation extraction. The highest yields in all UAE experiments were obtained at 80°C (Table 3), this is because at higher temperatures diffusion coeffi cient and generally solubility of solute are increased [Palma & Taylor, 1999].…”

Section: Effect Of Ultrasound-assisted Extraction On Phenolic Profi Lmentioning

confidence: 91%

High Voltage Electrical Discharges and Ultrasound-Assisted Extraction of Phenolics from Indigenous Fungus-Resistant Grape By-Product

Lončarić¹,

Jozinović²,

Kovač³

et al. 2020

Pol. J. Food Nutr. Sci.

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fl avonols, fl avan-3-ols, and anthocyanins [Kontić et al., 2016; Ehrhardt et al., 2014]. The FGR were bred by interspecifi c cross-breeding between the Mediterranean variate Vitis vinifera and American varieties with the aim to develop varieties resistant to fungal diseases, including powdery and downy mildews, and grey rot. FRG were introduced in Europe in the 19 th century to counter the invasion of the vermin Phylloxera (Viteus vitifoliae) [Noah, 2016]. Although production of wine from some FRG including the Noah, Othello, Isabelle, Jacquez, Clinton, and Herbemont varieties is forbidden due to Regulation EU No. 1308/2013, FRG are still grown in several European countries. Fungus-resistant grapes are consumed mainly as fresh fruit; however, due to their high resistance and high yields, they represent a good raw material for the production of different products such as juices, jams, jellies, etc. The GP obtained after production of such products from FRG has a high content of phenolic compounds due to incomplete extraction during the processing. These phenolic compounds are of interest because they have a positive effect on human health through their antioxidant, antibacterial, and anticarcinogenic potential, and the capability to prevent cardiovascular diseases, etc. [Rasouli et al., 2017]. Nowadays, there is a trend in exploring non-conventional, green methods for the extraction of phenolic compounds. High voltage electrical discharges (HVED) is one of them. The HVED enhance the rate of extracted phenolic compounds per initial plant material at low energy input during processing [

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“…The DPPH radical scavenging activity was determined according to the method of Wang et al () with some modification. About 2 ml of DPPH (200 μM, dissolved in 95% of ethanol), 1 ml of hydrolysate (10 mg/ml), and 2 ml of PBS (phosphate‐buffered saline) (0.1 M, pH 8.0) were mixed completely, then were kept in the dark for 30 min at 25°C.…”

Section: Methodsmentioning

confidence: 99%

Radical scavenging activities of peptide from Asian clam ( Corbicula fluminea ) and its protective effects on oxidative damage induced by hydrogen peroxide in HepG2 cells

Hao

Jiang

et al. 2020

J Food Biochem

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The soft part of Asian clam (Corbicula fluminea) was hydrolyzed using trypsin and the hydrolysates were separated by ultrafiltration using the membrane with molecular weight cutoff of 10 and 5 kDa. Three fractions (F10000, F10000–5000, and F5000) were obtained. The F5000 showed the strongest scavenging abilities to DPPH (2,2‐diphenyl‐1‐picrylhydrazyl), superoxide anion, and hydroxyl radicals, and was further fractionated into four fractions (component I, II, III, and IV) by gel chromatography. The protective effects of these components on oxidative damage induced by hydrogen peroxide (H2O2) in HepG2 cells were evaluated. Meanwhile, component I separated from F5000 had more positive effects on increasing the activity of catalase, decreasing lipid peroxidation, inhibiting H2O2‐induced apoptosis, and higher yield. The component I was further separated using anion exchange chromatography and reversed phase high‐performance liquid chromatography, and the peptide sequence of Lys‐Gly‐Pro‐Ala‐ Pro‐Phe‐Tyr‐Pro‐Leu was identified by mass with molecular weight of 988.3 Da. Practical applications Asian clam (C. fluminea) is used for liver protection in traditional Chinese medicine. The present study investigated the radical scavenging activity of the hydrolysates from C. fluminea and the protective effects of the hydrolysate fractions on oxidative damage induced by H2O2 in HepG2 cells. In addition, a peptide was purified from the hydrolysate and the amino acid sequence of it was identified. Although chemical antioxidant has some side effects on health, the peptide with antioxidant activity obtained from C. fluminea would have more extensive application in food and nutraceutical.

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Ultrasound-negative pressure cavitation extraction of phenolic compounds from blueberry leaves and evaluation of its DPPH radical scavenging activity

Cited by 56 publications

References 42 publications

Influence of Extraction Conditions on Ultrasound-Assisted Recovery of Bioactive Phenolics from Blueberry Pomace and Their Antioxidant Activity

Influence of Extraction Conditions on Ultrasound-Assisted Recovery of Bioactive Phenolics from Blueberry Pomace and Their Antioxidant Activity

High Voltage Electrical Discharges and Ultrasound-Assisted Extraction of Phenolics from Indigenous Fungus-Resistant Grape By-Product

Radical scavenging activities of peptide from Asian clam ( Corbicula fluminea ) and its protective effects on oxidative damage induced by hydrogen peroxide in HepG2 cells

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