Ultrasound Sonication Effects on Silk Fibroin Protein

Samal, Sangram Keshari; Kaplan, David L.; Chiellini, Emo

doi:10.1002/mame.201200377

Cited by 61 publications

(59 citation statements)

References 50 publications

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“…A promising biomaterial, silk fibroin protein (SFP), has been extensively studied and also approved by the US Food and Drug Administration for use in some biomedical applications . As a natural protein based biomaterial, it has good mechanical properties, low inflammatory reactions, good permeability, and good blood compatibility.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Mineralization of Silk Scaffolds

Samal

Dash

Declercq

et al. 2014

Macromolecular Bioscience

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The present study focuses on the alkaline phosphatase (ALP) mediated formation of apatitic minerals on porous silk fibroin protein (SFP) scaffolds. Porous SFP scaffolds impregnated with different concentrations of ALP are homogeneously mineralized under physiological conditions. The mineral structure is apatite while the structures differ as a function of the ALP concentration. Cellular adhesion, proliferation, and colonization of osteogenic MC3T3 cells improve on the mineralized SFP scaffolds. These findings suggest a simple process to generate mineralized scaffolds that can be used to enhanced bone tissue engineering-related utility.

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Section: Introductionmentioning

confidence: 99%

Enzymatic Mineralization of Silk Scaffolds

Samal

Dash

Declercq

et al. 2014

Macromolecular Bioscience

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“…68 It has been shown that ultrasound high frequency treatment of SFas mediated by the piezo crystalincreased crystallinity. 69 We speculate that the increase of crystallinity as a result of the use of the piezo crystal for droplet generation was sufficient to increase the crystallinity to an extent, such that sustained release profiles were found for IGF-I. Further increase in crystallinity by water vapor exposure or methanol treatment did not further impact the profile as compared to untreated SF microparticles.…”

Section: Acs Biomaterials Science and Engineeringmentioning

confidence: 89%

Pulmonary Insulin-like Growth Factor I Delivery from Trehalose and Silk-Fibroin Microparticles

Schultz

Vollmers

Lühmann

et al. 2015

ACS Biomater. Sci. Eng.

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Insulin-like growth factor I (IGF-I) is a strong anabolic peptide with promising therapeutic value in muscle wasting diseases such as sarcopenia. We report a pulmonary IGF-I delivery system deploying silk-fibroin (SF) as carrier and in comparison to trehalose. Both IGF-I delivery systems were characterized regarding IGF-I integrity, IGF-I release profiles and aerodynamic properties. Transepithelial in vitro transport of IGF-I using the pulmonary Calu-3 model cell system followed comparable kinetics and mechanism of uptake as earlier demonstrated for insulin (INS), for which effective pulmonary delivery is known. Microparticles were spray-dried using either trehalose or SF, resulting in geometries allowing alveolar deposition. The effective IGF-I shuttling through the epithelial barrier of the lung was demonstrated in an ex vivo human lung lobe model, and expanded the exciting possibility of this administration route to this effective and anabolic peptide.

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“…concentration, temperature, mechanical strength, chemical treatment, ultrasound sonication, and composition (Kim et al, 2015;Samal et al, 2013;Wang et al, 2008;Kweon et al, 2000;Kang et al, 2000;Ayub et al, 1994). Among them, sonication induced rapid gelation by reduction of molecular weight and β-sheet conformation (Samal et al, 2013).…”

Section: Gelation Of Sf Depends On a Number Of Parameters Includingmentioning

confidence: 99%

“…Among them, sonication induced rapid gelation by reduction of molecular weight and β-sheet conformation (Samal et al, 2013).…”

Section: Gelation Of Sf Depends On a Number Of Parameters Includingmentioning

confidence: 99%

Structural and thermal characteristics of photocrosslinked silk fibroin - PEG hydrogel

Jo¹,

Lee²,

Bragg³

et al. 2016

International Journal of Industrial Entomology

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Hydrogels are crosslinked hydrophilic matrices for a variety of biomedical applications. Silk fibroin (SF), one of typical natural biomaterials, has been explored as base material for hydrogel. Photocrosslinked SF hydrogel containing poly(ethylene glycol) (PEG) was formulated through visible light initiated thiol-acrylate photopolymerization. The morphological, structural and thermal properties of SF -PEG hydrogel was investigated through scanning electron microscopy, X-ray diffractometry, thermogravimetry, and differential scanning calorimetry. The morphology of SF hydrogel showed dot and uneven surface with network cross-section. X-ray diffraction curves showed that the specific diffraction peaks of PEG were not changed by the intensity of the peaks were affected by sonication. Thermo-degradation behavior of SF -PEG hydrogel sonicated was significantly affected and became complex pattern compared to unsonicated ones. However, the melting endothermic temperature of SF -PEG hydrogel was not changed but the crystalline enthalpy was decreased by gelation and sonication. IntroductionHydrogels are crosslinked hydrophilic matrices suitable for a variety of biomedical applications, including tissue engineering matrix, contact lenses, drug delivery carriers and cell therapy Lin and Anseth, 2009;Kweon et al., 2001;Hanawa et al., 1995). A variety of natural and synthetic polymers have been explored as the base materials for hydrogel crosslinking. Silk fibroin (SF) produced by Bombyx mori silkworm can be formulated into physical hydrogels or be modified to display reactive motifs (e.g., methacrylate) for chemical crosslinking (Yecel et al., 2014;Kim et al., 2014;Kasoju and Bora, 2012;Kweon et al., 2001;Kweon et al., 2000).SF can also form β-sheets structure, which can self-assemble into higher order structures with enhanced stability. On the other hand, hydrogels prepared from derivatives of poly(ethylene glycol) (PEG) chemically crosslinked with limited degradability (Lin, 2015;Lin and Anseth, 2009;Lutolf and Hubbell, 2005).

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Ultrasound Sonication Effects on Silk Fibroin Protein

Cited by 61 publications

References 50 publications

Enzymatic Mineralization of Silk Scaffolds

Enzymatic Mineralization of Silk Scaffolds

Pulmonary Insulin-like Growth Factor I Delivery from Trehalose and Silk-Fibroin Microparticles

Structural and thermal characteristics of photocrosslinked silk fibroin - PEG hydrogel

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