Ultrasound stimulation attenuates resorption of tooth root induced by experimental force application

Inubushi, Toshihiro; Tanaka, Eiji; Braga, Emanuel; Ohtani, Junji; Kawazoe, Aki; Tanne, Kazuo; Miyauchi, Mutsumi; Takata, Takashi

doi:10.1016/j.bone.2013.01.021

Cited by 42 publications

(37 citation statements)

References 47 publications

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“…Moreover, in an in vivo experiment with rats, LIPUS inhibited the resorption of tooth roots (Inubushi et al, 2013). This result agreed with our data obtained with fish scales.…”

Section: Discussionsupporting

confidence: 83%

Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound

Suzuki

Hanmoto

Yano

et al. 2016

Comparative Biochemistry and Physiology Part A: Molecular &

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Using fish scales in which osteoclasts and osteoblasts coexist on the calcified bone matrix, we examined the effects of low-intensity pulsed ultrasound (LIPUS) on both osteoclasts and osteoblasts. At 3 hours of incubation after LIPUS treatment, osteoclastic markers such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA expressions decreased significantly while mRNA expressions of osteoblastic markers, osteocalcin, distal-less homeobox 5, runt-related transcription factor 2a, and runt-related transcription factor 2b, increased significantly. At 6 and 18 hours of incubation, however, both osteoclastic and osteoblastic marker mRNA expression did not change at least present conditions. Using GeneChip analysis of zebrafish scales treated with LIPUS, we found that cell death-related genes were upregulated with LIPUS treatment. Real-time PCR analysis indicated that the expression of apoptosis-related genes also increased significantly. To confirm the involvement of apoptosis in osteoclasts with LIPUS, osteoclasts were induced by autotransplanting scales in goldfish. Thereafter, the DNA fragmentation associated with apoptosis was detected in the osteoclasts using the TUNEL (TdTmediated dUTP nick-end labeling) method. The multi-nuclei of TRAP-stained osteoclasts in the scales were labeled with TUNEL. TUNEL staining showed that the number of apoptotic osteoclasts in goldfish scales was significantly elevated by treatment with LIPUS at 3 hours of incubation. Thus, we are the first to demonstrate that LIPUS directly functions to osteoclasts and to conclude that LIPUS directly causes apoptosis in osteoclasts shortly after exposure.Key words: LIPUS, osteoclasts, osteoblasts, fish scales, apoptosis, GeneChip analysis Abbreviations: ALP, alkaline phosphatase; Bcl-2, B-cell lymphoma 2; CTSK, Cathepsin K; Dlx5, distal less homeobox5; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; LIPUS, low-intensity pulsed ultrasound; OCN, osteocalcin; PMAIP, phorbol-12-myristate-13-acetateinduced protein 1; SAFHS, sonic accelerated fracture healing system; pNP, para-nitrophenol; TRAP, tartrate-resistant acid phosphatase; TUNEL, TdT-mediated dUTP nick end labelling; TNFRSF10A, tumor necrosis factor receptor superfamily, member a; Runx2a, runt related transcription factor 2a; Runx2b, runt related transcription factor 2b 3

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“…Moreover, in an in vivo experiment with rats, LIPUS inhibited the resorption of tooth roots (Inubushi et al, 2013). This result agreed with our data obtained with fish scales.…”

Section: Discussionsupporting

confidence: 83%

Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound

Suzuki

Hanmoto

Yano

et al. 2016

Comparative Biochemistry and Physiology Part A: Molecular &

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“…moderate bone resorption is necessary to promote osteogenesis. Furthermore, regarding RANKL and OPG mRNA expression following LIPUS treatment, a similar phenomenon has been reported in a mouse cementoblast cell line (7). Therefore, the data of mammalian hard tissue support our present results.…”

Section: Acknowledgmentssupporting

confidence: 81%

<b>Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of </b><b>bone </b>

et al. 2017

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The effects of low-intensity pulsed ultrasound (LIPUS) on osteoclastogenesis were examined using fish scales that had both osteoclasts and osteoblasts. The binding of the receptor activator of NF-κB ligand (RANKL) in osteoblasts to the receptor activator of NF-κB (RANK) in osteoclasts induced osteoclastogenesis. Therefore, we focused on RANK/RANKL signaling. After 6 h of incubation following LIPUS treatment, mRNA expression of RANKL increased significantly. Resulting from the increased RANKL mRNA level, the expression of transcription-regulating factors significantly increased after 6 h of incubation, and then the mRNA expression of functional genes was significantly up-regulated after 12 h of incubation. However, the mRNA expression of osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, also significantly increased after 6 h of incubation and tended to further increase after 12 h of incubation. At 24 h of incubation, osteoclastic functional genes' mRNA expression decreased to the level of the control. Furthermore, we performed an in vivo experiment with goldfish. Two weeks after daily LIPUS exposure, osteoclastic marker enzymes tended to decrease while osteoblastic marker enzymes were activated. The regeneration rate of the LIPUS-treated scales was significantly higher than that of the control scales. Thus, LIPUS moderately activates osteoclasts and induces bone formation.Low-intensity pulsed ultrasound (LIPUS), a noninvasive remedial measure, promotes the repair of bone fracture and distraction osteogenesis (see a review, 25). Until now, the study of LIPUS has focused on osteoblastic growth and differentiation (3,8,10). However, the details of the direct effect of

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“…16,23,24,29 In addition, it has been demonstrated that LIPUS can inhibit osteoclast activity by decreasing the Receptor activator of nuclear factor kappa-B ligand/Osteoprotegerin (RANKL/OPG) ratio. 23,24,30 This could also be due to the anti-inflammatory effect of LIPUS, 31 as it has been reported to decrease the tumor necrosis factor-a or interleukin-1b 31 that are also involved in the proliferation and differentiation of odontoclast cells. 32 Our findings are in accordance with previous reports 15,25,30,31 that showed significantly fewer RL in LIPUS-treated teeth compared to control teeth, except for at the distal surface.…”

Section: Discussionmentioning

confidence: 99%

“…23,24,30 This could also be due to the anti-inflammatory effect of LIPUS, 31 as it has been reported to decrease the tumor necrosis factor-a or interleukin-1b 31 that are also involved in the proliferation and differentiation of odontoclast cells. 32 Our findings are in accordance with previous reports 15,25,30,31 that showed significantly fewer RL in LIPUS-treated teeth compared to control teeth, except for at the distal surface. The distal surface in the LIPUS group showed a lower number of RL compared to the control; however, this difference was not significant.…”

Section: Discussionmentioning

confidence: 99%

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Raza

Major

Dederich

et al. 2015

The Angle Orthodontist

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Objectives: To evaluate the effects of low-intensity pulsed ultrasound (LIPUS) on orthodontically induced tooth root resorption caused by torque in human subjects. Materials and Methods: Ten healthy patients (12-35 years of age) who required extraction of all first premolars as a part of their routine orthodontic treatment were recruited. A 15u twist was applied in the arch wire using 0.019 3 0.025-inch TMA in a 0.022-inch bracket system (Synergy R) that produced a buccal root torque of approximately 5 N/mm at the bracket level. Using a split mouth design, randomization, and blinding, one side of the arch received LIPUS for 20 minutes per day for 4 weeks at an incident intensity of 30 mW/cm 2 of the transducers' surface area. The other side served as a self-control, which received a sham transducer. After 4 weeks, all first premolars were extracted and micro-computed tomographic analysis was performed on these extracted teeth. A linear mixed-model statistical analysis was used. Results: LIPUS-treated teeth showed significantly less total volume of resorption lacunae compared to control teeth by a mean difference of (0.54 6 0.09 mm 3 ) (P , .001) and percentage of root resorption by a mean difference of (0.33 6 0.05 mm 3 ) (P , .001). In addition, significantly fewer resorption lacunae were found on all root surfaces in the LIPUS group compared to the control except in the instance of the distal surface. Limitations: This study was performed on limited number of cases during a 4-week period. Conclusions: LIPUS minimizes root resorption when applied during torque tooth movement over a 4-week period. (Angle Orthod. 2016;86:550-557.)

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Ultrasound stimulation attenuates resorption of tooth root induced by experimental force application

Cited by 42 publications

References 47 publications

Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound

Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound

<b>Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of </b><b>bone </b>

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

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