Ultrasound Treatment Increases Transfection Efficiency of Low Molecular Weight Chitosan in Fibroblasts but Not in KB Cells

Kedjarune‐Leggat, Ureporn; Chanyapat, Supaprutsakul; Chotigeat, Wilaiwan

doi:10.1371/journal.pone.0092076

Cited by 6 publications

(4 citation statements)

References 41 publications

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“…Finally, the resultant mixture was added to the cells in each well. The medium was replaced with serum-containing medium 5 h after transfection (33,34).…”

Section: Methodsmentioning

confidence: 99%

C5a promotes the proliferation of human nasopharyngeal carcinoma cells through PCAF-mediated STAT3 acetylation

et al. 2014

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The anaphylatoxin C5a is a chemoattractant that can induce various inflammatory responses in vivo via the C5a receptor (C5aR). There is emerging evidence that C5a is generated in the cancer microenvironment. However, the role of C5a in human nasopharyngeal carcinoma (NPC) remains largely unclear. Thus, the present study aimed to examine the direct influence of C5a stimulation on the proliferation of human NPC cells and to identify the underlying molecular mechanisms. The effects of C5a stimulation on the proliferation of human NPC cells were studied in vitro, and P300/CBP-associated factor (PCAF)‑mediated signal transducer and activator of transcription 3 (STAT3) acetylation and its role in regulating the proliferation of NPC cells was subsequently explored. Our results demonstrated that C5a stimulation increased the proliferation of human NPC cells in vitro. STAT3 acetylation was further found to be enhanced in human NPC cells induced by C5a. Moreover, PCAF induction was required for STAT3 acetylation in human NPC cells by exposure to C5a. Functionally, PCAF-mediated STAT3 acetylation contributed to the proliferation of human NPC cells stimulated by C5a. These results illustrate the novel activity of the C5a-C5aR axis that promotes human NPC cell proliferation through PCAF‑mediated STAT3 acetylation. This may provide a potential strategy for treating human NPC through inhibition of C5a or its receptors.

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“…Finally, the resultant mixture was added to the cells in each well. The medium was replaced with serum-containing medium 5 h after transfection (33,34).…”

Section: Methodsmentioning

confidence: 99%

C5a promotes the proliferation of human nasopharyngeal carcinoma cells through PCAF-mediated STAT3 acetylation

et al. 2014

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“…We also only tested one degree of deacetylation of chitosan. Other forms of chitosan have also been successful in enhancing transfection [ 53 , 54 ] and thus may offer other forms of expression. We did not examine different chitosan particle formulations, nor look extensively at particle stability to determine potential effects on DNA plasmid complexation and delivery.…”

Section: Discussionmentioning

confidence: 99%

Chitosan Particles Complexed with CA5-HIF-1α Plasmids Increase Angiogenesis and Improve Wound Healing

Born,

Bengali,

Hsu

et al. 2023

IJMS

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Wound therapies involving gene delivery to the skin have significant potential due to the advantage and ease of local treatment. However, choosing the appropriate vector to enable successful gene expression while also ensuring that the treatment’s immediate material components are conducive to healing itself is critical. In this study, we utilized a particulate formulation of the polymer chitosan (chitosan particles, CPs) as a non-viral vector for the delivery of a plasmid encoding human CA5-HIF-1α, a degradation resistant form of HIF-1α, to enhance wound healing. We also compared the angiogenic potential of our treatment (HIF/CPs) to that of chitosan particles containing only the plasmid backbone (bb/CPs) and the chitosan particle vector alone (CPs). Our results indicate that chitosan particles exert angiogenic effects that are enhanced with the human CA5-HIF-1α-encoded plasmid. Moreover, HIF/CPs enhanced wound healing in diabetic db/db mice (p < 0.01), and healed tissue was found to contain a significantly increased number of blood vessels compared to bb/CPs (p < 0.01), CPs (p < 0.05) and no-treatment groups (p < 0.01). Thus, this study represents a method of gene delivery to the skin that utilizes an inherently pro-wound-healing polymer as a vector for plasmid DNA that has broad application for the expression of other therapeutic genes.

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“…Microbubbles can be made more effective through their covalent attachment to the recipient cells' surface [37]. Sonoporation can be combined with chemical methods of transfection [38]. Directing the transfer of the vector particles by focusing the ultrasonic waves to the target tissue is a practical option [39,40].…”

Section: Ultrasound-assisted Delivery Of Mrnamentioning

confidence: 99%

Methods of Transfection with Messenger RNA Gene Vectors

Tolmachov¹,

Tolmachova²

2015

Gene Therapy - Principles and Challenges

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depends on the efficient interaction of all three parties. Thus, the vector should be customised for the target cell population and presented in a form that is resistant to the aggressive factors in the delivery milieu. At the same time, the delivery environment should be adjusted to be more vector-friendly and more cell-friendly. The recipient cells should be subjected to a specific regimen or artificially modified to become receptive to gene transfer with a particular vector and resistant to the environment. As a rule, barriers outside tissues e.g. mucus and an aggressive

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Ultrasound Treatment Increases Transfection Efficiency of Low Molecular Weight Chitosan in Fibroblasts but Not in KB Cells

Cited by 6 publications

References 41 publications

C5a promotes the proliferation of human nasopharyngeal carcinoma cells through PCAF-mediated STAT3 acetylation

C5a promotes the proliferation of human nasopharyngeal carcinoma cells through PCAF-mediated STAT3 acetylation

Chitosan Particles Complexed with CA5-HIF-1α Plasmids Increase Angiogenesis and Improve Wound Healing

Methods of Transfection with Messenger RNA Gene Vectors

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