Ultrastructural analysis in yeast reveals a meiosis-specific actin-containing nuclear bundle

Такаги, Таичиро; Osumi, Masako; Shinohara, Atsushi

doi:10.1038/s42003-021-02545-9

Cited by 12 publications

(9 citation statements)

References 51 publications

(81 reference statements)

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“…A recent study also reported novel meiotic intranuclear filaments in yeast cells [ 44 ], though it is unclear if those filaments are triple-helical. Nevertheless, the similar abundance and bundling phenotypes suggest that the filaments observed by Takagi, et al .…”

Section: Resultsmentioning

confidence: 99%

“…In contrast, Takagi et al . provided immuno-EM evidence that the similar filaments they saw (probably MTHs) contain actin [ 44 ]. While MTHs cannot be F-actin, which would appear double helical, they could instead be a non-canonical triple-helical polymer of actin that is sensitive to Lat-A and is bound by anti-actin antibodies in immuno-EM experiments, but unable to bind rhodamine-phalloidin and Lifeact.…”

Section: Discussionmentioning

confidence: 99%

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Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast

et al. 2022

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In meiosis, cells undergo two sequential rounds of cell division, termed meiosis I and meiosis II. Textbook models of the meiosis I substage called pachytene show that nuclei have conspicuous 100-nm-wide, ladder-like synaptonemal complexes and ordered chromatin loops. It remains unknown if these cells have any other large, meiosis-related intranuclear structures. Here we present cryo-ET analysis of frozen-hydrated budding yeast cells before, during, and after pachytene. We found no cryo-ET densities that resemble dense ladder-like structures or ordered chromatin loops. Instead, we found large numbers of 12-nm-wide triple-helices that pack into ordered bundles. These structures, herein called meiotic triple helices (MTHs), are present in meiotic cells, but not in interphase cells. MTHs are enriched in the nucleus but not enriched in the cytoplasm. Bundles of MTHs form at the same timeframe as synaptonemal complexes (SCs) in wild-type cells and in mutant cells that are unable to form SCs. These results suggest that in yeast, SCs coexist with previously unreported large, ordered assemblies.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast

et al. 2022

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“…It should be noted, that some studies also report polymerized filamentous actin in the nucleus of diverse organisms, however, its structural properties are still under-researched, and outside of the animal kingdom this issue has received little attention to date [73][74][75][76][77][78]. For reviews see [79][80][81].…”

Section: The Relation Between Actin and Chromatin Remodelling: Arp8 A...mentioning

confidence: 99%

Effects of Environmental Stress Factors on the Actin Cytoskeleton of Fungi and Plants: Ionizing Radiation and ROS

Stephan

2023

Preprint

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Actin is an abundant and multifaceted protein in eukaryotic cells that has been detected in the cytoplasm as well as in the nucleus. In cooperation with numerous interacting accessory-proteins, monomeric actin (G-actin) polymerizes into microfilaments (F-actin) which constitute ubiquitous subcellular higher order structures. Considering the extensive spatial dimensions and multifunctionality of actin super-arrays, the present study analyses the issue if and to what extent environmental stress factors, specifically ionizing radiation (IR) and reactive oxygen species (ROS), affect the cellular actin-entity. In that context, this review particularly surveys IR-response of fungi and plants. It examines in detail which actin-related cellular constituents and molecular pathways are influenced by ionizing radiation and related ROS. This comprehensive survey concludes that the general integrity of the total cellular actin cytoskeleton is a requirement for IR-tolerance. Actin´s functions in genome organization and nuclear events like chromatin remodelling, DNA-repair, and transcription play a key role. Beyond that, it is highly significant that the macromolecular cytoplasmic and cortical actin-frameworks are affected by IR as well. In response to IR, actin-filament bundling proteins (fimbrins) are required to stabilize cables or patches. In addition, the actin-associated factors mediating cellular polarity are essential for IR-survivability. Moreover, it is concluded that a cellular homeostasis system comprising ROS, ROS-scavengers, NADPH-oxidases, and the actin cytoskeleton plays an essential role here. Consequently, besides the actin-fraction which controls crucial genome-integrity, also the portion which facilitates orderly cellular transport and polarized growth has to be maintained in order to survive IR.

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“…It should be noted, that some studies also report polymerized filamentous actin in the nucleus of diverse organisms, however, its structural properties are still under‐researched, and outside of the animal kingdom this issue has received little attention to date (Andrin et al, 2012; Belin, Lee, & Mullins, 2015; Lestourgeon, Forer, Yang, Bertram, & Pusch, 1975; McDonald, Carrero, Andrin, de Vries, & Hendzel, 2006; Schoenenberger et al, 2005; Takagi, Osumi, & Shinohara, 2021). For reviews see (Castano et al, 2010; Gettemans et al, 2005; Pederson & Aebi, 2002).…”

Section: Actin‐related Ir‐sensitive Yeast Deletion Mutantsmentioning

confidence: 99%

Effects of environmental stress factors on the actin cytoskeleton of fungi and plants: Ionizing radiation and ROS

Stephan

2023

Cytoskeleton

View full text Add to dashboard Cite

Actin is an abundant and multifaceted protein in eukaryotic cells that has been detected in the cytoplasm as well as in the nucleus. In cooperation with numerous interacting accessory‐proteins, monomeric actin (G‐actin) polymerizes into microfilaments (F‐actin) which constitute ubiquitous subcellular higher order structures. Considering the extensive spatial dimensions and multifunctionality of actin superarrays, the present study analyses the issue if and to what extent environmental stress factors, specifically ionizing radiation (IR) and reactive oxygen species (ROS), affect the cellular actin‐entity. In that context, this review particularly surveys IR‐response of fungi and plants. It examines in detail which actin‐related cellular constituents and molecular pathways are influenced by IR and related ROS. This comprehensive survey concludes that the general integrity of the total cellular actin cytoskeleton is a requirement for IR‐tolerance. Actin's functions in genome organization and nuclear events like chromatin remodeling, DNA‐repair, and transcription play a key role. Beyond that, it is highly significant that the macromolecular cytoplasmic and cortical actin‐frameworks are affected by IR as well. In response to IR, actin‐filament bundling proteins (fimbrins) are required to stabilize cables or patches. In addition, the actin‐associated factors mediating cellular polarity are essential for IR‐survivability. Moreover, it is concluded that a cellular homeostasis system comprising ROS, ROS‐scavengers, NADPH‐oxidases, and the actin cytoskeleton plays an essential role here. Consequently, besides the actin‐fraction which controls crucial genome‐integrity, also the portion which facilitates orderly cellular transport and polarized growth has to be maintained in order to survive IR.

show abstract

Ultrastructural analysis in yeast reveals a meiosis-specific actin-containing nuclear bundle

Cited by 12 publications

References 51 publications

Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast

Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast

Effects of Environmental Stress Factors on the Actin Cytoskeleton of Fungi and Plants: Ionizing Radiation and ROS

Effects of environmental stress factors on the actin cytoskeleton of fungi and plants: Ionizing radiation and ROS

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