Ultrastructural and autometallographic studies of the nephrocytes of Lithobius forficatus L. (Myriapoda, Chilopoda): role in detoxification of cadmium and lead

Vandenbulcke, Franck; Grelle, C.; Fabre, M.; Descamps, M.

doi:10.1016/s0020-7322(98)00034-8

Cited by 20 publications

(13 citation statements)

References 21 publications

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“…Another alteration which has been observed in the current study was the severe vacuolation in the cytoplasm due to the action of acid hydrolases that has been released from the lysosomes (Vandenbulcke et al, 1998;Polidori et al, 2018) which is accompanied by changes in the PH of the cytosol. Moreover, alterations in cytoplasmic organelles such as swallow of the mitochondria and dilation of the rough and smooth endoplasmic reticulum may be a result of the radiation which disrupts cytoplasmic membranes as reported earlier by Kheirallah (2016) and Kheirallah et al (2017).…”

Section: Discussionsupporting

confidence: 63%

Midgut cells alteration in gamma-irradiated beetles (Blaps polycresta, Coleoptera: Tenebrionidae)

Kheirallah

El-Samad

2020

Braz. J. Biol.

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Abstract This study was conducted to examine the effect of gamma radiation on biological specimens. Thus, our concept is to clarify that exposure to accumulated dose of 0.2 Gy gamma rays (0.66 rad/Sec. dose rate) from Cs137 source induces cellular perturbations in the midgut epithelium of the F1 progeny of Blaps polycresta, therefore affecting nutrition and growth. Beetles were reared in laboratory conditions and the newly emerged adults were irradiated with the aforementioned dose. Histological and ultrastructure anomalies of midgut cells (digestive and regenerative cells) were observed by 72 h after radiation exposure to ensure that the cells will not return to control state. Retardation in the development of the F1 progeny was also noticed and beetles died through two weeks. In the light of these observations, biological tissue act as an indicator to the continuous exposure to environmental radiation.

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Section: Discussionsupporting

confidence: 63%

Midgut cells alteration in gamma-irradiated beetles (Blaps polycresta, Coleoptera: Tenebrionidae)

Kheirallah

El-Samad

2020

Braz. J. Biol.

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“…High levels of heavy metals can be sequestered as dense vesicles of the lysosomal system (Lauverjat et al, 1989). The continuous release of lysosomal hydrolase may also result in vacuolated areas in the cytoplasm (Vandenbulcke et al, 1998). Sperm agglutination was noticed in our micrographs, which might be a result of antisperm antibodies attachment.…”

Section: Discussionmentioning

confidence: 70%

Nickel oxide nanoparticles induce genotoxicity and cellular alterations in the ground beetle Blaps polycresta (Coleoptera: Tenebrionidae)

et al. 2021

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Nickel nanoparticles (Ni-NPs) have advantageous applications in the industry; however, little is known of their adverse effects on biological tissues. In the present study, the ground beetle Blaps polycresta was employed as a sensitive indicator for nickel oxide nanoparticles (NiO-NPs) toxicity. Adult male beetles were injected with six dose levels of NiO-NPs (0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 mg/g body weight). Mortality was reported daily over 30 days under laboratory conditions to establish an LD50. Nickel was detected in the testicular tissues of the beetles using X-ray analysis and transmission electronic microscopy. Beetles treated with the sublethal dose of 0.02 mg/g were selected to observe molecular, cellular, and subcellular changes. Gene transcripts of HSP70, HSP90, and MT1 were found to be increased >2.5-, 1.5-, and 2-fold, respectively, in the treated group compared with the controls. Decreased gene expression of AcPC01, AcPC02, and AcPC04 (≤1.5-, ≤2-, and < 2.5-fold, respectively, vs. controls) also were reported in the treated group. Under light microscopy, various structural changes were observed in the testicular tissues of the treated beetles. Ultrastructure observations using scanning and transmission electron microscopy showed severe damage to the subcellular organelles as well as deformities of the heads and flagella of the spermatozoa. Therefore, the present study postulated the impact of NiO-NPs in an ecological model.

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“…(Meyhoefer et al 1985, Welsch & Rehkaemper 1987, Meyhofer & Morse 1996, Lambert et al 1998. Their role in the storage and/or detoxification of heavy metals has been suggested in various aquatic species (Dillaman 1980, Giamberini & Pihan 1996, Soto et al 1996, Vandenbulcke et al 1998. The peripheral pedicels of nephrocytes are similar to those of the podocytes of the cells lining the Bowman space in the kidney of vertebrates and through which a plasma ultrafiltrate is formed (Ruppert 1994).…”

Section: Discussionmentioning

confidence: 99%

Bioaccumulation of inorganic and methylated mercury by the gills of the shore crab Carcinus maenas: transepithelial fluxes and histochemical localization

Laporte¹,

Truchot

Mesmer-Dudons³

et al. 2002

Mar. Ecol. Prog. Ser.

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In order to better understand the role of the gills in the accumulation of contaminants, branchial uptake and bioaccumulation of inorganic (HgCl 2 ) and monomethylmercury (CH 3 HgCl) were quantified in the shore crab Carcinus maenas, using living animals and an in vitro perfused gill preparation exposed to 50 µg l -1 of either chemical forms in the external medium. In addition, localization of accumulated mercury was studied using the histochemical autometallographic technique by both light and electron microscopy. Gill tissue strongly accumulated either inorganic or methylated mercury at similar levels in vitro and in vivo. For both chemical forms of the metal, only about 1% of the total mercury input was recovered in the effluent fluid from in vitro perfused gills and could thus be considered available to distribute inside the animal via the circulatory system. Inorganic Hg was histochemically found to be accumulated at 2 markedly different locations in the gills: at the cuticular surface in direct contact with the contaminated medium and at high local levels in the central vacuole of gill nephrocytes. Although also present at these 2 locations, methylmercury was distributed more diffusely and more evenly in all cells. These results suggest that the high affinity of gill tissue for both forms of mercury may confer on this organ the role of an external barrier strongly limiting the invasion of the metal toward other compartments of the body.

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Ultrastructural and autometallographic studies of the nephrocytes of Lithobius forficatus L. (Myriapoda, Chilopoda): role in detoxification of cadmium and lead

Cited by 20 publications

References 21 publications

Midgut cells alteration in gamma-irradiated beetles (Blaps polycresta, Coleoptera: Tenebrionidae)

Midgut cells alteration in gamma-irradiated beetles (Blaps polycresta, Coleoptera: Tenebrionidae)

Nickel oxide nanoparticles induce genotoxicity and cellular alterations in the ground beetle Blaps polycresta (Coleoptera: Tenebrionidae)

Bioaccumulation of inorganic and methylated mercury by the gills of the shore crab Carcinus maenas: transepithelial fluxes and histochemical localization

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