Ultrastructural and autoradiographical analysis show a faster skin repair in He–Ne laser-treated wounds

Araújo, Carlos E.N. de; Ribeiro, Martha S.; Favaro, Rodolfo R.; Zezéll, Denise Maria; Zorn, Telma Maria Tenório

doi:10.1016/j.jphotobiol.2006.08.006

Cited by 56 publications

(49 citation statements)

References 31 publications

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“…It is assumed that the laser stimulates the transformation of fibroblasts in early myofibroblasts 6 . Araújo et al 3 observed that the irradiated dermis showed a high number of fibroblasts, and these, we found a large amount of collagen vesicles and well developed endoplasmic reticulum, indicating increased activity compared to control group. Reddy 16 showed a 14% increase in collagen deposition in wounds of diabetic rats irradiated compared to non-irradiated.…”

Section: Discussionsupporting

confidence: 62%

“…This finding is consistent with the results of the work of Walker et al 14 , which showed that the InGaAlP laser had a positive effect in neoformation of vessels, especially in the initial formation of granulation tissue. Admit that cells of the healing process respond the lasers that operate in the red spectral range, and this is mainly observed for the stimulation of angiogenesis 3 . In this study, neovascularization demonstrated is directly related to the activity of the laser on skin wounds.…”

Section: Discussionmentioning

confidence: 99%

“…Effects of low intensity laser on healing have been developed, checking analgesic, antiinflammatory and healing [3][4][5] , although many studies don't show the specific type of laser used, refer only to such as laser 600nm 5 . The laser aluminumgallium-indium-phosphorus (InGaAlP) has been widely used in LBI because of their power output of 30mW, which can reduce the stimulation of the irradiated site compared with other lasers such as helium-neon (He-Ne) and gallium arsenide (GaAs) 6 .…”

Section: Introductionmentioning

confidence: 99%

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Healing activity of laser InGaAlP (660nm) in rats

Tacon¹,

Santos²,

Parente³

et al. 2011

Acta Cir. Bras.

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PuRPOsE: To evaluate the effect the healing activity of diode laser Indium Gallium Aluminum Phosphorus (InGaAlP) ë660nm on healing of surgical wounds in rats.METHOds: Fifty-four female Wistar rats were used, divided into three groups (n=18) and subdivided into three subgroups (n=6) to be studied in 5, 10 and 15 th days after surgical procedures. The wound was induced in the dorsal-cervical using punch. The lesions were irradiated on alternate days with InGaAlP laser, the energy densities of 3J/cm 2 (L3) or 6J/cm 2 (L6). The control group received no irradiation. At 5, 10 and 15 th days after surgery the animals were euthanized and the repair area was removed and histological sections were stained with hematoxylin-eosin and picrossírius. We evaluated macroscopic and histological lesions in the times cited, as well as morphometric analysis of angiogenesis and collagen content. REsuLTs:The wound healing activity InGaAlP laser was evidenced by increased angiogenesis group L3 and L6 in relation to control group (CG) at the 5 th day (p=0.0001) and decreased polymorphonuclear infiltrate and hemorrhage (p=0.045 and p=0.07 respectively) in the groups L3 and L6 in relation to control group (GC). On the 10 and 15 th days was also observed in groups treated with laser L3 and L6 stimulation was pronounced fibroplasia (p=0.0003 and p=0.034 respectively) when compared with the control group (CG). CONCLusION:The InGaAlP laser acted positively on the healing of skin wounds in rats.Keywords: Wound Healing. Laser Therapy, Low Intensity. Rats. REsuMOOBJETIVO: Avaliar a atividade cicatrizante do laser diodo Alumínio Gálio Índio Fósforo (AlGaInP), ë660nm em feridas cutâneas induzida em ratos. MéTOdOs:Foram utilizados 54 ratos Wistar, fêmeas, divididos em três grupos (n=18) e subdivididos em três subgrupos (n=6) animais para serem estudados no 5, 10 e 15° dias após o procedimento cirúrgico. A ferida foi induzida em região dorso-cervical utilizando punch.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Healing activity of laser InGaAlP (660nm) in rats

Tacon¹,

Santos²,

Parente³

et al. 2011

Acta Cir. Bras.

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“…The low cost of the equipment combined with promising results has generated great interest among researchers in using this technique for the modulation of tissue repair. Promising results have been observed with the use of photobiomodulation therapy (PBMT) in skin wound healing, including decreased inflammatory activity (DE ARAÚJO et al, 2007), modulation of inflammatory mediators (FIORIO et al, 2017), neovascularization (LORETI et al, 2015;NOVAES et al, 2014) and faster tissue repair (REDDY; STEHNO-BITTEL;ENWEMEKA, 2001;NOVAES et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effect of gallium arsenide low-level laser therapy on the inflammatory phase of skin wound healing in rats

Carvalho

Bizo

Toma

et al. 2017

AVB

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Article historyThe use of photobiomodulation therapy (PBMT) has shown promising results for the inflammatory modulation of tissue repair. This study aimed to evaluate the antiinflammatory potential of gallium arsenide (GaAs) PBMT in skin wound healing via (1) measurement of the temperature on the surface of the skin wound; (2) white blood cell count; and (3) histopathological examination. Skin lesions were induced on the dorsum of 20 Wistar rats, after which the animals were divided into two groups: a treatment group, subjected to GaAs PBMT, and a control group. Thermography was performed daily in all of the study animals until the end of the experiment. On the fourth day after lesion induction, whole blood was collected (white blood cell count), animals were euthanized, and skin lesions were biopsied (histopathological examination). There were no differences in the number of leukocytes and in the histopathological evaluations between the groups; however, the thermography analysis indicated an increase in temperature in the treated group. The antiinflammatory activity of GaAs PBMT was not confirmed. The increase in temperature on the surface of the skin lesions after LLLT requires further elucidation because this result could not be justified by the direct action of PBMT.

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“…[14][15][16] Previous studies in our laboratory as well as others have suggested that photo-stimulation by laser seems to occur during the inflammatory and proliferative phases of wound healing, exactly because of their influence on biomodulation of eicosanoids, especially PGs, and on the oxidative enzyme system of the skin. 1,2,5,[11][12][13][14][15][16][17][18][19] Consequently, studies that show the effects of combined treatments in wound healing are opportune, since both laser therapy and the use of COX inhibitors act during the same time period in the repair process, that is, both separate treatments affect the inflammatory and proliferative phases of skin healing in animals and humans. Considering this scenario, the purpose of this pre-clinical study was to evaluate the isolated and combined effects of low level laser therapy (LLLT) with helium-neon (He-Ne, 632nm) and selective inhibition of cyclooxygenase-2 (COX-2) in the reepithelialization of skin-incisional wounds in mice.…”

Section: Introductionmentioning

confidence: 99%

Uso combinado da laserterapia de baixa potência e da inibição da ciclooxigenase-2 na reepitelização de ferida incisional em pele de camundongos: um estudo pré-clínico

Santuzzi

Buss

Pedrosa³

et al. 2011

An. Bras. Dermatol.

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Abstract:BACKGROUND: Low level laser therapy and cyclooxygenase-2 (ICOX2) selective inhibitors have been widely used to modulate inflammatory response; however, their effect on wound reepithelialization are not well understood. OBJECTIVE: To evaluate the isolated and combined effects of low level laser therapy and ICOX2 in the reepithelization of skin incisional wounds in mice. METHODS: We induced a 1-cm wound on the back of each mouse, which were divided into four groups (N = 20): control, laser therapy, treated with celecoxib and combined therapy. The animals in the celecoxib and combined therapy groups were treated with celecoxib for 10 days before skin incision. The experimental wounds were irradiated with He-Ne low power laser (632nm, dose: 4J/cm2) in scanning for 12 seconds during three consecutive days in the laser therapy and combined therapy groups. The animals were sacrificed 3 days after surgery. Samples of the wounds were collected and stained (Masson's Trichrome) for histomorphometric analysis. RESULTS: Both the laser therapy group and the celecoxib group showed an increase in skin reepithelialization compared to the control group; however, the combined therapy group showed no differences. As for keratinization, the laser therapy and combined therapy groups showed a reduction in keratinocytes compared with the control group. CONCLUSION: The results show that the use of low level laser therapy and ICOX2 in isolation increases epithelial cells, but only low level laser therapy reduced skin keratinocytes. The combined treatment restores innate epithelialization and decreases keratinization in spite of accelerating wound contraction with improvement in the organization of the wound in the skin of mice. Keywords: Cyclooxygenase 2 inhibitors; Epithelial cells; Keratinocytes; Laser therapy; Wound healing Resumo: FUNDAMENTOS: A laserterapia de baixa potência e os inibidores seletivos da ciclooxigenase-2 (ICOX2) vem sendo muito utilizados para modular a resposta inflamatória, entretanto, os seus efeitos na reepitelização de feridas não são bem compreendidos. OBJETIVO: Avaliar os efeitos isolados e combinados da laserterapia de baixa potência e da ICOX2 na reepitelização de ferida incisional na pele de camundongos. MÉTODO: Foi induzida uma ferida de 1 cm no dorso de cada camundongo, que foram divididos em quatro grupos (N=20): Controle, Laserterapia, Tratados com celecoxib e Terapia conjugada. Os animais dos grupos celecoxib e Terapia conjugada foram tratados com celecoxib por 10 dias antes da incisão cutânea. As feridas experimentais foram irradiadas com laserterapia de baixa potência He-Ne (632nm, dose: 4J/cm2) em varredura, por 12 segundos durante três dias consecutivos nos grupos Laserterapia e Terapia conjugada. Os animais foram sacrificados no 3º dia de pós-operatório. Amostras das feridas foram coletadas e coradas (Tricromio de Masson) para análise histomorfométrica. RESULTADOS: Tanto o grupo Laserterapia, quanto o grupo celecoxib, mostrou aumento da reepitelização cutânea em relação ao grupo Controle,...

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Ultrastructural and autoradiographical analysis show a faster skin repair in He–Ne laser-treated wounds

Cited by 56 publications

References 31 publications

Healing activity of laser InGaAlP (660nm) in rats

Healing activity of laser InGaAlP (660nm) in rats

Effect of gallium arsenide low-level laser therapy on the inflammatory phase of skin wound healing in rats

Uso combinado da laserterapia de baixa potência e da inibição da ciclooxigenase-2 na reepitelização de ferida incisional em pele de camundongos: um estudo pré-clínico

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