Ultrastructural and histological characteristics of the equine endometrium at day 5 post ovulation

Caballeros, Jorge Emilio; Camacho, Cesar Augusto; Cazales, N.; Estradé, María José; Rechsteiner, Sandra Mara da Encarnação Fiala; Jobim, Mariana; Mattos, Rodrigo Costa

doi:10.1016/j.theriogenology.2019.04.006

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…Eosinophils were reported to be more common on Day 5 PP in the mare [23]. The presence of eosinophils could be supposed to represent an immune response in the early postpartum period, as previously suggested for the initial interaction between the conceptus and the uterus in the mare [25]. The seldom observation of small mononucleate cell aggregates, only at PP Days 7-14, seems to suggest a minor role of these cells in the process of uterine involution in jennies.…”

Section: Discussionsupporting

confidence: 55%

Postpartum Uterine Involution in Martina Franca Jennies

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Marruchella

et al. 2021

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This study investigated the postpartum (PP) uterine involution in nine multiparous Martina Franca jennies with at term, normal, and singleton foaling. Transrectal ultrasonography performed at Days 1, 3, 7, 14, 21, and 28 showed that the uterine tip diameters did not differ between the post-pregnant (PPH) and non-post-pregnant uterine horns (NPPH), whereas the diameter of the middle PPH was larger than the NPPH until Day 7 (p < 0.05). The diameter of the corpora-cornual junction resulted larger in the PPH than NPPH at Day 7 (p < 0.05). At Day 3, the microcaruncolae were not detected. Endometrial glands (GL) number increased, with the highest value on Day 28. Endometrial GL area and perimeter decreased (p < 0.001) from Day 1 to Day 28. Epithelial thickness increased from Days 1–3 to Day 7 (p < 0.001), and concurrently with the foal heat to Day 14 (p < 0.001), with a decrease at Days 21–28. A marked neutrophils reduction on Day 7 and eosinophil increase from the first three days to Days 7–14 was observed. The results suggest that, in Martina Franca jennies with normal foaling, the PP uterine involution can be considered complete on Day 14.

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Section: Discussionsupporting

confidence: 55%

Postpartum Uterine Involution in Martina Franca Jennies

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Fusi

Marruchella

et al. 2021

Animals

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“…Infusion with conceptus fragments showed endometrial and vascular changes associated with a protein stimulus (Camacho et al, 2018), suggesting similarity with early equine pregnancy process (Caballeros et al, 2019;Camozzato et al, 2019). Proteins, reported in the present research, were identified in current studies of proteins and transcripts of endometrial tissues, embryonic tissues, and uterine fluid (Klein et al, 2010;Swegen et al, 2017;Maloney et al, 2018;Smits et al, 2018;Bastos et al, 2019) (Figure 6).…”

Section: Discussionsupporting

confidence: 71%

“…Infusion of conceptus fragments reported histomorphometric changes, an increase of glandular secretion, and immune cells in the uterus (Camacho et al, 2018). SERPINB1 signalization may partially mediate these alterations in the early pregnancy (Martínez, 2016;Caballeros et al, 2019;Camozzato et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Many approaches have been used to improve understanding of the stage of the estrous cycle, uterine receptivity, embryo-maternal communication, and embryo metabolism. Thus, histomorphometric (Keenan et al, 1987;Caballeros et al, 2019;Camozzato et al, 2019) and molecular analysis (Merkl et al, 2010;Klein and Troedsson, 2011b;Swegen et al, 2017;Smits et al, 2018;Bastos et al, 2019) have been performed using equine endometrial tissues, uterine fluid, and embryos or conceptus fragments.…”

Section: Introductionmentioning

confidence: 99%

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Uterine infusion of conceptus fragments changes the protein profile from cyclic mares

et al. 2020

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This experiment aimed to compare at day seven after ovulation, the protein profile of uterine fluid in cyclic mares with mares infused two days before with Day 13 conceptus fragments. Experimental animals were ten healthy cyclic mares, examined daily to detect ovulation (Day 0) as soon as estrus was confirmed. On day seven, after ovulation, uterine fluid was collected, constituting the Cyclic group (n = 10). The same mares were examined in the second cycle until ovulation was detected. On day five, after ovulation, fragments from a previously collected concepti were infused into each mare's uterus. Two days after infusion, uterine fluid was collected, constituting the Fragment group (n = 10). Two-dimensional electrophoresis technique processed uterine fluid samples. A total of 373 spots were detected. MALDI-TOF/TOF and NanoUHPLC-QTOF mass spectrometry identified twenty spots with differences in abundance between the Cyclic and Fragment group. Thirteen proteins were identified, with different abundance between groups. Identified proteins may be related to embryo-maternal communication, which involves adhesion, nutrition, endothelial cell proliferation, transport, and immunological tolerance. In conclusion, conceptus fragments signalized changes in the protein profile of uterine fluid seven days after ovulation in comparison to the observed at Day 7 in the same cyclic mares.

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“…In pregnant mares, the number of leukocytes increased in association with a local immunological response ( Keenan et al, 1987 ). The endometrium of inseminated mares’ present ultra-structural and histological changes with an increase of lymphocytes on the 5 th day after ovulation before the embryo enters the uterus ( Caballeros et al, 2019 ). Embryo presence was associated with increased blood flow and vascular perfusion from the uterine artery on the 11 th day after ovulation ( Bollwein et al, 2003 ) and to local vascular changes related to embryo localization ( Silva et al, 2005 ).‬‬‬‬‬‬‬‬‬…”

Section: Introductionmentioning

confidence: 99%

Histomorphometric and vascular changes in equine endometrium after the infusion of conceptus fragments

et al. 2020

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This experiment aimed to verify if the proteins present in a 13 th day conceptus induce changes in the equine endometrial ultra-structure, histology, and vascularization, two days after its infusion. Ten healthy cyclic mares were used. Once estrus was confirmed, mares were examined daily to detect ovulation (day 0). After ovulation, mares were examined daily until day seven by transrectal palpation and B-mode and Doppler ultrasonography. In this first cycle, intrauterine biopsies were collected at day seven after ovulation, constituting the Cyclic group (n = 10). In the second cycle, the same mares daily were examined until ovulation was detected. After ovulation, mares were examined daily by transrectal palpation and B-mode and Doppler ultrasonography until day 7. On day 5, after ovulation, fragments from previously collected 13-day-old concepti were infused into the uterus of each mare. Intrauterine biopsies were collected at day 7 in all mares (n = 10), constituting the Fragment group. The percentage of ciliated and flattened cells decreased in the Fragment group. Protruded cells, superficial and intraglandular secretion, glandular lumen and diameter, blood vessel diameter, endometrial vascularization, and immune cells were higher in the Fragment group than in the Cyclic group. In summary, proteins of 13 th day equine conceptus fragments infused at day five after ovulation signaled histological and vascular changes in the endometrium at the 7 th day after ovulation.

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Ultrastructural and histological characteristics of the equine endometrium at day 5 post ovulation

Cited by 5 publications

References 21 publications

Postpartum Uterine Involution in Martina Franca Jennies

Postpartum Uterine Involution in Martina Franca Jennies

Uterine infusion of conceptus fragments changes the protein profile from cyclic mares

Histomorphometric and vascular changes in equine endometrium after the infusion of conceptus fragments

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