Ultrastructural artefacts in biopsied normal myocardium and their relevance to myocardial biopsy in man.

Olmesdahl, P J; Gregory, Michael; Cameron, Evan Wm.

doi:10.1136/thx.34.1.82

Cited by 21 publications

(8 citation statements)

References 9 publications

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“…Patient I also had Wolff-ParkinsonWhite syndrome, and patient 13 In 50% of the patients in this study, the biopsy specimens showed changes characteristic of a nonspecific cardiomyopathy, which are indistinguishable from alterations seen in biopsy specimens from patients with idiopathic, dilated, congestive cardiomyopathy. 1' 7 Despite the severity of the histologic abnormalities, heart size, resting cardiac function, and exercise tolerance were well-preserved in these patients.…”

Section: Resultsmentioning

confidence: 52%

Results of endomyocardial biopsy in patients with spontaneous ventricular tachycardia but without apparent structural heart disease.

Strain¹,

Grose²,

Factor³

et al. 1983

Circulation

192

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To evaluate possible occult myocardial disease in 18 patients whose only major manifestation of heart disease was spontaneous ventricular tachycardia or fibrillation, right ventricular endomyocardial biopsies were performed. None of the patients had symptoms of ischemic or congestive heart disease, and at catheterization none had significant lesions of the coronary arteries or regional wall motion abnormalities of the left ventricle. The mean left ventricular ejection fraction (65 + 7%), mean right ventricular ejection fraction (55 + 9%), mean cardiac index (3.0 ± 0.5 1/min/m2), mean right atrial pressure, mean pulmonary capillary wedge pressure, and mean pulmonary artery systolic pressure were normal. However, right ventricular endomyocardial biopsy specimens were abnormal in 16 of 18 (89%) patients: nine (50%) had changes of a significant, although nonspecific, cardiomyopathy with myocellular hypertrophy, interstitial and perivascular fibrosis, and vascular sclerosis; three (17%) had subacute inflammatory myocarditis; two (11%) had diffuse abnormalities of the intramyocardial arteries; and two (11%) had pathologic changes consistent with arrhythmogenic right ventricular dysplasia. In the two (1 %) patients with normal biopsy specimens, one had Wolff-Parkinson-White syndrome and the other had mitral valve prolapse. Although histologic abnormalities were found in 89% of these patients, performance of right ventricular endomyocardial biopsies in this group of patients should be considered a research procedure. We conclude that the majority of patients who have serious ventricular arrhythmias but no apparent structural cardiac abnormalities have abnormal right ventricular biopsy specimens and that the arrhythmias may be the first manifestation of a variety of primary myocardial abnormalities.

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Section: Resultsmentioning

confidence: 52%

Results of endomyocardial biopsy in patients with spontaneous ventricular tachycardia but without apparent structural heart disease.

Strain¹,

Grose²,

Factor³

et al. 1983

Circulation

192

View full text Add to dashboard Cite

show abstract

“…Our biopsy material was rarely spoiled by artifacts. We therefore do not share the pessimism of Eckner et al 40 and Olmesdahl et al 41 regarding the problem of bioptome-induced artifacts in the interpretation of endomyocardial biopsy.…”

Section: Reproducibility Of Electron Microscopic Evaluationmentioning

confidence: 99%

Electron microscopic investigation of endomyocardial biopsy samples in hypertrophy and cardiomyopathy. A semiquantitative study in 48 patients.

Baandrup¹,

Florio²,

Roters³

et al. 1981

Circulation

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SUMMARY Electron microscopic and statistical analyses of 66 right ventricular biopsies from 48 patients were undertaken to investigate whether quantitative differences exist between those patients with "ordinary" myocardial hypertrophy and those suffering from a form of cardiomyopathy.The electron microscopic changes were scored and correlated with hemodynamic variables such as ejection fraction (EF), left ventricular end-diastolic pressure (LVEDP) and length of history. The patients were followed for an average of 22.5 months, permitting an assessment of prognosis.The results show that the three diagnostic groups "ordinary" hypertrophy, hypertrophic cardiomyopathy (HOCM) and congestive cardiomyopathy (COCM) overlap, but crossover of sarcomeres is more frequent in patients in whom HOCM is diagnosed. Except for a tenuous relationship between EF, and the electron microscopy (EM) (r = -0.46,p < 0.1) and between LVEDP and EM score (r = 0.61,p < 0.01), in the COCM group, no correlation could be established between EF, LVEDP and length of history when the patients were grouped according to histologic or clinical diagnosis.This study shows that the various claims regarding relationships between morphologic changes and the functional status of patients or prognosis cannot be confirmed.

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“…After desensitisation, the central artefact-free core of the biopsy specimen was removed as described by Olmesdahl et al 33 and diced into 1 mm cubes before re-immersion in fixative for a further 1 hour. After postfixation/staining with 1% osmium tetroxide, the tissue was dehydrated through increasing concentrations of ethanol before being embedded in Spurr epoxy resin.…”

Section: Electron Microscopymentioning

confidence: 99%

Mobilisation of satellite cells following ischaemia and reperfusion in primate skeletal muscle

Gregory

Mars

2004

SA J. Sports Med.

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Objective. To describe the morphological and morphometric features of activated skeletal muscle satellite cells in primates, using an ischaemic reperfusion model. Setting. The study was undertaken at the Biomedical Resource Centre and the Electron Microscopy Unit of the University of KwaZulu-Natal. Interventions. Eight vervet monkeys were anaesthetised and subjected to 3 hours of tourniquet-induced lower limb ischaemia. Open muscle biopsies were taken from tibialis anterior muscle immediately after tourniquet release and 12, 24, 36 and 48 hours after tourniquet release. Control biopsies were taken from the opposite limb. Main outcome measures. Description of the morphological and morphometric changes in satellite cells after activation, as seen on transmission electron microscopy. Results. Two distinct patterns of satellite cell activation are described. In group 1, the cytoplasm of the satellite cell expands around the myocyte and the gap between the satellite cell and the myocyte appears to break down, or in group 2, the novel observation of the satellite cell breaking away from the myofibre and becoming a myocyte totally encased in its own basal lamina. The satellite cells of group 1 were significantly longer than the group 2 cells (p = 0.018) and this was associated with a significant reduction in the percentage of nuclear to cell area (p = 0.011). Conclusions. Tourniquet-induced ischaemic reperfusion injury is shown to result in two distinct patterns of satellite cell activation which may represent different functions or subsets of satellite cells.

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Ultrastructural artefacts in biopsied normal myocardium and their relevance to myocardial biopsy in man.

Cited by 21 publications

References 9 publications

Results of endomyocardial biopsy in patients with spontaneous ventricular tachycardia but without apparent structural heart disease.

Results of endomyocardial biopsy in patients with spontaneous ventricular tachycardia but without apparent structural heart disease.

Electron microscopic investigation of endomyocardial biopsy samples in hypertrophy and cardiomyopathy. A semiquantitative study in 48 patients.

Mobilisation of satellite cells following ischaemia and reperfusion in primate skeletal muscle

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