Ultrastructural changes in hepatocellular carcinoma cells induced by exponential pulses of nanosecond duration delivered via a transmission line

Yin, Shengyong; Liu, Zhen; Mashayekh, Amir Shahriar; Guo, Danjing; Qian, Junjie; Wang, Yübo; Deng, Guanlei; Zheng, Chao; Zhenhong, MA; Zhou, Lin; Yan, Keping; Zheng, Shu

doi:10.1016/j.bioelechem.2020.107548

Cited by 8 publications

(5 citation statements)

References 52 publications

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“…As previously shown, cell swelling may reduce their numbers [ 15 ] and modify the cell surface properties since microvilli serve as a membrane reservoir [ 30 ]. Under the influence of nsPEF, this phenomenon has been previously observed, but a stronger electric field and more pulses were used for the two human hepatocellular carcinoma (HCC) cell line treatments, and it finally led to additional thermal effects [ 31 ].…”

Section: Discussionmentioning

confidence: 94%

“…The first phenomenon was likely caused by membrane permeabilization, especially since the influx of Ca 2+ from the external environment, as well as the release of calcium ions from the intracellular stores caused by nsPEF [ 7 ], make linking PS with AnV easier [ 61 ]. Some researchers also believe that negatively charged PS can be pushed from the inner to the outer leaflet of the membrane driven by an electric potential across the membrane [ 31 ]. Within 60 min, the proportion of viable AnV - /Pi - cells did not decrease visibly; however, propidium iodide penetrated the AnV + cells over time, increasing the proportion of the AnV + /PI + cell fraction.…”

Section: Discussionmentioning

confidence: 99%

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Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

Kasprzycka

Trębińska-Stryjewska

Lewandowski

et al. 2021

IJMS

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The purpose of this study was to verify whether the nanosecond pulsed electric field, not eliciting thermal effects, permanently changes the molecular processes and gene expression of Leydig TM3 cells. The cells were exposed to a moderate electric field (80 quasi-rectangular shape pulses, 60 ns pulse width, and an electric field of 14 kV/cm). The putative disturbances were recorded over 24 h. After exposure to the nanosecond pulsed electric field, a 19% increase in cell diameter, a loss of microvilli, and a 70% reduction in cell adhesion were observed. Some cells showed the nonapoptotic externalization of phosphatidylserine through the pores in the plasma membrane. The cell proportion in the subG1 phase increased by 8% at the expense of the S and G2/M phases, and the DNA was fragmented in a small proportion of the cells. The membrane mitochondrial potential and superoxide content decreased by 37% and 23%, respectively. Microarray’s transcriptome analysis demonstrated a negative transient effect on the expression of genes involved in oxidative phosphorylation, DNA repair, cell proliferation, and the overexpression of plasma membrane proteins. We conclude that nanosecond pulsed electric field affected the physiology and gene expression of TM3 cells transiently, with a noticeable heterogeneity of cellular responses.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

Kasprzycka

Trębińska-Stryjewska

Lewandowski

et al. 2021

IJMS

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show abstract

“…Under transmission electron microscopy, pulsed tumor cells exhibited signs of apoptosis: the chromatin in the nucleus was condensed, the micro-villi on the free surface of the cell membrane became thinner and reduced, the cytoplasm was condensed, the rough endoplasm reticulum expanded and swelled into vesicles, and the mitochondria became vacuolated. Previous studies [22,23] have found that nsPEFs with short pulse duration have a variety of effects on cells. Nanosecond pulses destroy the ultra-structure of the plasma membrane by affecting the charged molecules on its surface.…”

Section: Discussionmentioning

confidence: 99%

Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner

Liu

Dong

et al. 2023

PLoS ONE

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Background Liver tumor remains an important cause of cancer-related death. Nanosecond pulsed electric fields (nsPEFs) are advantageous in the treatment of melanoma and pancreatic cancer, but their therapeutic application on liver tumors need to be further studied. Methods Hep3B cells were treated with nsPEFs. The biological behaviors of cells were detected by Cell Counting Kit-8, 5-ethynyl-20-deoxyuridine, and transmission electron microscopy (TEM) assays. In vivo, rabbit VX2 liver tumor models were ablated by ultrasound-guided nsPEFs and radiofrequency ablation (RFA). Contrast-enhanced ultrasound (CEUS) was used to evaluate the ablation effect. HE staining and Masson staining were used to evaluate the tissue morphology after ablation. Immunohistochemistry was performed to determine the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin at different time points after ablation. Results The cell viability of Hep3B cells was continuously lower than that of the control group within 3 days after pulse treatment. The proliferation of Hep3B cells was significantly affected by nsPEFs. TEM showed that Hep3B cells underwent significant morphological changes after pulse treatment. In vivo, CEUS imaging showed that nsPEFs could completely ablate model rabbit VX2 liver tumors. After nsPEFs ablation, the area of tumor fibrosis and the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin were decreased. However, after RFA, rabbit VX2 liver tumor tissue showed complete necrosis, but the expression of PCNA and α-smooth muscle actin did not decrease compared to the tumor group. Conclusions nsPEFs can induce Hep3B cells apoptosis and ablate rabbit VX2 liver tumors in a non-thermal manner versus RFA. The ultrasound contrast agent can monitor immediate effect of nsPEF ablation. This study provides a basis for the clinical study of nsPEFs ablation of liver cancer.

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“…In vivo ablation of rat liver cancer models, Nuccitelli et al [ 16 ] observed rapid swelling of mitochondria, endoplasmic reticulum, and Golgi apparatus. The formation of irregular holes in the plasma membrane is caused by the pulsed electric field acting on charged molecules in the plasma membrane [ 24 ]. In addition, the decrease of mitochondrial membrane potential and the release of cytochrome may play an important role in cell apoptosis [ 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Nanosecond Pulsed Electric Fields in Cell Vitality, Apoptosis, and Proliferation of TPC-1 Cells

Liu

Zou

Sun

et al. 2021

Analytical Cellular Pathology

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Objective. To evaluate the effects of nanosecond pulsed electric fields (nsPEFs) with different pulse durations in cell vitality, apoptosis, and proliferation of TPC-1 cells, optimize pulse parameters and expand the application range of nsPEFs. Methods. The pulse duration of 0, 300 ns, 500 ns, and 900 ns is generated with nsPEF generator. CCK-8 was used to investigate the effect of nsPEFs on the viability of TPC-1 cells. Flow cytometry was used to evaluate the apoptosis of TPC-1 after pulse treatment. The effect of nsPEFs on the proliferation ability of TPC-1 cells was detected by 5-ethy-nyl-2 ′ -deoxyuridine. The morphological changes of TPC-1 cells after pulse treatment were observed by transmission electron microscopy. Results. NsPEFs with 900 ns pulse duration can significantly affect the viability of TPC-1 cells and inhibit the proliferation ability of TPC-1 cells. In addition, nsPEFs can also induce apoptosis of TPC-1 cells. Conclusion. NsPEFs with longer pulse duration can significantly affect the biological behavior of TPC-1 cells, such as cell viability and proliferation ability, and can also induce cell apoptosis, thereby inhibiting cell growth.

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Ultrastructural changes in hepatocellular carcinoma cells induced by exponential pulses of nanosecond duration delivered via a transmission line

Cited by 8 publications

References 52 publications

Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner

Effects of Nanosecond Pulsed Electric Fields in Cell Vitality, Apoptosis, and Proliferation of TPC-1 Cells

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