Ultrastructural changes in mitochondria during oogenesis in two phylogenetically close fish species

Charitonidou, Katerina; Panteris, Emmanuel; Ganias, Konstantinos

doi:10.1002/jmor.21456

Cited by 2 publications

(4 citation statements)

References 33 publications

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“…These differences in organelle distribution between the fish species could be due to different functional demands during the development of oocytes. Sardine and anchovy oocytes showed cytoplasmic changes in early and mid‐secondary oocytes, as well as differences in mitochondrial ultrastructure at different oocyte stages (Charitonidou et al ., 2022). The absence of oil/lipid droplets from the cytoplasm of anchovy oocytes, as well as the absence of lipid droplet fusion during the oocyte maturation phase, which occurs in sardine oocytes that contain lipid droplets, are two important distinctions during oocyte development between these species.…”

Section: Discussionmentioning

confidence: 99%

“…Both species have a cystovarian ovary and are multiple spawners with indeterminate fecundity, producing many batches of eggs over prolonged periods (Ganias, 2014). Despite these similarities, a recent study from our group (Charitonidou et al ., 2022) has unveiled important differences in the ultrastructure of mitochondria of primary and secondary growth oocytes between the two studied species. We hereby examined changes in cytoplasmic organization after the disassembly of the Bb in early secondary growth oocytes, in terms of organelle distribution ( e.g ., mitochondria, endoplasmic reticulum, etc .…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 98%

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Balbiani body formation and cytoplasmic zonation during early oocyte development in two Clupeiform fishes

Charitonidou

Panteris

Ganias

2022

Journal of Fish Biology

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The Balbiani body (Bb) was examined in primary growth phase oocytes for the first time in two clupeoid fish species, the Mediterranean sardine, Sardina pilchardus, and the European anchovy, Engraulis encrasicolus, which belong to different families, Clupeidae and Engraulidae, respectively. Cytoplasmic morphological changes of early secondary growth oocytes were also investigated using confocal laser scanning microscopy, light and transmission electron microscopy. The ultrastructural observations showed that the two species develop a distinct spherical Bb. However, differences in the cytoplasm, mainly in the perinuclear area, were observed. Briefly, in sardine the Bb coexists with a thick perinuclear ring containing mitochondria, nuage, endoplasmic reticulum and small vesicles, while in anchovy this perinuclear ring is thinner, consisting of complexes of nuage and mitochondria. After the disassembly of the Bb, a prominent cytoplasmic zonation develops in the secondary growth oocytes of sardine and anchovy, although with different organelle distribution between the two species. Sardine oocytes exhibit a thick zone of endoplasmic reticulum around the nucleus, whereas in those of anchovy, a thick mitochondria‐rich ring surrounding the nucleus was observed. The cytoplasmic characteristics, such as the perinuclear ring in primary oocytes in sardine and the mitochondria‐rich ring of early secondary oocytes in anchovy, are also discernible in histological sections by standard procedures and could thus be used as indicators of maturity or imminent spawning period in routine light microscopy observations, providing a valuable tool for applied fisheries biology.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 98%

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Balbiani body formation and cytoplasmic zonation during early oocyte development in two Clupeiform fishes

Charitonidou

Panteris

Ganias

2022

Journal of Fish Biology

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“…Lepomis macrochirus had a high relative abundance in the traditional survey but a low value in the eDNA survey, whereas C. Cuvieri showed the opposite trend (Figure 2). The rate of eDNA release and number of mitochondria contained in a single cell may vary among fish species (Charitonidou et al, 2022), leading to differences in the relationship between individual numbers and eDNA copy numbers across species. The time interval between eDNA surveys and traditional surveys and the natural changes in fish community structure may also explain this change.…”

Section: Quantitative Metabarcodingmentioning

confidence: 99%

Monitoring of multiple fish species by quantitative environmental DNA metabarcoding surveys over two summer seasons

Wu,

Osugi,

Inagawa

et al. 2023

Molecular Ecology Resources

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Periodic monitoring can provide important information for the protection of endangered fish, sustainable use of fishery resources and management of alien species. Previous studies have attempted to monitor fish using non‐invasive environmental DNA (eDNA) technology, generally employing quantitative PCR to quantify the eDNA concentration. However, the throughput was limited. High‐throughput metabarcoding technology can detect the DNA of multiple species simultaneously in a single experiment but does not provide sufficient quantification. In this study, we applied a quantitative metabarcoding approach to simultaneously quantify the eDNA concentration of an entire fish assemblage in a small reservoir over two summer seasons. Traditional surveys were also conducted to investigate the individuals of fish. The eDNA concentrations were quantified using quantitative metabarcoding, and the fish species detected using this approach were highly consistent with the results of traditional fish monitoring. A significant positive relationship was observed between the eDNA concentration and fish species abundance. Seasonal changes in fish community structure were estimated using eDNA concentrations, which may reveal the activity seasons of different fish. The eDNA concentrations of different fish species peaked at different water temperatures, reflecting the differential responses of fish species to this environmental factor. Finally, by detecting outlier eDNA concentrations, the spawning activities of 13 fish species were estimated, 12 of which were roughly consistent with the current knowledge of fish spawning periods. These results indicate that quantitative eDNA metabarcoding with dozens of sampling times is useful for the simultaneous ecological monitoring of multiple fish species.

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Ultrastructural changes in mitochondria during oogenesis in two phylogenetically close fish species

Cited by 2 publications

References 33 publications

Balbiani body formation and cytoplasmic zonation during early oocyte development in two Clupeiform fishes

Balbiani body formation and cytoplasmic zonation during early oocyte development in two Clupeiform fishes

Monitoring of multiple fish species by quantitative environmental DNA metabarcoding surveys over two summer seasons

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