1998
DOI: 10.1007/s11626-998-0036-7
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Ultrastructural changes of Drosophila larval and prepupal salivary glands cultured in vitro with ecdysone

Abstract: Alterations in the ultrastructure of in vitro cultured larval salivary glands of Drosophila melanogaster in response to the steroid hormone ecdysone were studied in relation to complex changes in puffing patterns. We found that the changes in the fine structure of cultured glands reflected progression of the puffing pattern, and they paralleled those seen in vivo. We observed that glue secretion by exocytosis, the main function of salivary glands, took place between puff stage 5 (PS5) and PS7. Glue could not b… Show more

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Cited by 33 publications
(37 citation statements)
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“…Acidification of the vacuolar lumen by proton pumping vATPases was reported earlier as an essential factor for the release of iron (Fe 3+ ) from the dimerized transferrin during endocytosis (El Hage Chahine et al, 2012;Steere et al, 2012;Farkaš et al, 2015). In addition, the temporary enhancement of O 2 consumption after pupariation could be related to a temporary increase in the number of mitochondria in the cytoplasm of the SG cells (Farkaš and Šuťáková, 1998), which occurs over a relatively short time (duration 2-3 h) and is not accompanied by remarkable changes in the mitochondrial morphology.…”
Section: Relationships Between Respiratory Metabolism and Cytologicalmentioning
confidence: 93%
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“…Acidification of the vacuolar lumen by proton pumping vATPases was reported earlier as an essential factor for the release of iron (Fe 3+ ) from the dimerized transferrin during endocytosis (El Hage Chahine et al, 2012;Steere et al, 2012;Farkaš et al, 2015). In addition, the temporary enhancement of O 2 consumption after pupariation could be related to a temporary increase in the number of mitochondria in the cytoplasm of the SG cells (Farkaš and Šuťáková, 1998), which occurs over a relatively short time (duration 2-3 h) and is not accompanied by remarkable changes in the mitochondrial morphology.…”
Section: Relationships Between Respiratory Metabolism and Cytologicalmentioning
confidence: 93%
“…Therefore, in order to limit this impact of slow gas diffusion, we reduced the volume of the Grace's medium to 1-2 ll, displaced in a small, conical, 5 mm plastic Heraeus tissue culture cell permeable to respiratory gas. The Grace's tissue culture medium diluted 5:1 according to Farkaš and Šuťáková (1998), was purchased from Gibco Invitrogen Inc. The respiratory and compensatory vessels (1 or 2 ml) were made from disposable plastic syringes.…”
Section: Respirographic Recording Of O 2 Consumptionmentioning
confidence: 99%
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“…A number of studies on the PCD of the Drosophila salivary gland have been carried out over recent years (Jochovµ et al 1997;Jiang et al 1997;FarkaÐ and Mechler 2000;Lee and Baehrecke 2001;Lee et al 2002Lee et al , 2003. However, no detailed analysis of the process of tissue degradation itself has been made, because salivary glands degenerating in vivo are too fragile to be dissected out, and because the degeneration has never been completed under in vitro culture conditions even when mid-prepupal salivary glands were incubated with ecdysone (Jiang et al 1997;FarkaÐ and utµkovµ 1998). Here I establish an in vitro culture system that enables real-time observation of the cell destruction under an inverted microscope, using salivary glands from phanerocephalic pupae instead of cryptocephalic prepupae.…”
Section: Introductionmentioning
confidence: 99%