Ultrastructural changes seen after ALA‐IPL photorejuvenation: A pilot study

Marmur, Ellen S.; Phelps, Robert; Goldberg, David J.

doi:10.1080/147641700510037725

Cited by 90 publications

(58 citation statements)

References 17 publications

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“…However, there have been conflicting findings on the effect of ALA-PDT on the expression of MMPs and collagen fibers when PDT was used for photorejuvenation of photoaged skin. Marmur et al 7 showed, by conducting an ultrastructural analysis, that ALA-IPL induced an increase of type I collagen fibers in photodamaged skin. Additionally, the expressions of MMP-1, -3 and -12 were decreased and the immunoreactivity for TGF-β as well as for the TGF-β type II receptor in the epidermis was significantly increased after ALA-PDT 8 .…”

Section: Discussionmentioning

confidence: 99%

The Expressions of TGF-β₁and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment

et al. 2011

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Background: Topical photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) was originally used for treating superficial skin tumors. The application of PDT to other inflammatory dermatoses like acne vulgaris, psoriasis, granuloma annulare, localized scleroderma and lichen sclerosus has recently been introduced. However, the underlying mechanisms are not well understood. We've previously reported the induction of tumor growth factor (TGF)-β1 and interleukin (IL)-10 after PDT with ALA and intense pulsed light (IPL) in cultured HaCaT cells. Objective: The purpose of this study was to investigate the expressions of TGF-β1 and IL-10 in cultured fibroblasts after PDT with using ALA and IPL. Methods: Cultured fibroblasts were treated with ALA-IPL PDT (1μmol/L of ALA; 0, 4, 8 and 12 J/cm 2 of IPL). The expressions of TGF-β1 and IL-10 were investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. Results: TGF-β1 mRNA and protein were reduced down to 0.52-and 0.63-fold, respectively, after PDT and the IL-10 protein was increased up to 2.74-fold after PDT. Conclusion: The reduction of TGF-β1 was prominent after PDT and so an antisclerotic effect can be expected after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses.

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Section: Discussionmentioning

confidence: 99%

The Expressions of TGF-β₁and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment

et al. 2011

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“…However, most prior data have been based on clinical observation, without histopathologic confirmation. Recently, Marmur, Phelps, and Goldberg 15 showed that ALA-IPL induced increase of type I collagen fibers in the photodamaged skin by ultrastructural analysis. In addition, Orringer et al 16 also demonstrated increase of type I and III procollagen in the photoaged skin after ALA-PDL.…”

Section: Discussionmentioning

confidence: 99%

Photorejuvenation induced by 5-aminolevulinic acid photodynamic therapy in patients with actinic keratosis: A histologic analysis

Park

Sohn

Lee

et al. 2010

Journal of the American Academy of Dermatology

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Background: Repeated exposure to ultraviolet radiation from the sun results in premature photoaging. Photodynamic therapy (PDT) has been shown to be effective for treatment of photoaging, although the data from most studies have been based on clinical observation.Objectives: We investigated whether 5-aminolevulinic acid (ALA)-PDT induced histologic changes suggesting photorejuvenation.Methods: Fourteen patients with one to three actinic keratoses on the face were treated twice with ALA-PDT by using a 1200 W metal halogen lamp at 1-month intervals. Skin biopsy before and 1 month after the PDT was performed. Twenty-five pairs of specimens were obtained. We examined the specimens with routine and immunohistochemical staining and evaluated the parameters associated with photoaging by using image analysis.Results: After ALA-PDT, the mean epidermal thickness and dermal inflammatory infiltrate were reduced. The total collagen volume in the dermis significantly increased with expression of type I and III procollagen. The level of transforming growth factor b and transforming growth factor b type II receptors in the epidermis also increased. The elastotic material with co-localizing fibrillin-1 and tropoelastin expression in the dermis decreased after treatment. The expression of matrix metalloproteinases-1, -3, and -12 also decreased.Limitations: The study was limited by the small sample size.Conclusions: ALA-PDT resulted in histological changes indicating restoration of photoaged skin. These data suggest that ALA-PDT could be effective for photorejuvenation. ( J Am Acad Dermatol 2010;62:85-95.)

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“…154,155 Histopathologically, dilated blood vessels and increased pigment are found accumulated in the papillary dermis. [156][157][158][159][160] Current treatments for photorejuvenation include retinoids, laser resurfacing, chemical peels, and various light therapies (Table 3). Photorejuvenation has been frequently observed in patients undergoing PDT for other disease entities, and therefore has become an area of further investigation.…”

Section: Photoaging/antiaging/photorejuvenationmentioning

confidence: 99%

Current Evidence and Applications of Photodynamic Therapy in Dermatology

2015

Journal of the Dermatology Nurses' Association

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Abstract:In photodynamic therapy (PDT) a photosensitizer -a molecule that is activated by light -is administered and exposed to a light source. This leads both to destruction of cells targeted by the particular type of photosensitizer, and immunomodulation. Given the ease with which photosensitizers and light can be delivered to the skin, it should come as no surprise that PDT is an increasingly utilized therapeutic in dermatology. PDT is used commonly to treat precancerous cells, sun-damaged skin, and acne. It has reportedly also been used to treat other conditions including inflammatory disorders and cutaneous infections. This review discusses the principles behind how PDT is used in dermatology, as well as evidence for current applications of PDT.

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Ultrastructural changes seen after ALA‐IPL photorejuvenation: A pilot study

Cited by 90 publications

References 17 publications

The Expressions of TGF-β₁and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment

The Expressions of TGF-β₁and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment

Photorejuvenation induced by 5-aminolevulinic acid photodynamic therapy in patients with actinic keratosis: A histologic analysis

Current Evidence and Applications of Photodynamic Therapy in Dermatology

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Ultrastructural changes seen after ALA‐IPL photorejuvenation: A pilot study

Cited by 90 publications

References 17 publications

The Expressions of TGF-β1and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment

The Expressions of TGF-β1and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment

Photorejuvenation induced by 5-aminolevulinic acid photodynamic therapy in patients with actinic keratosis: A histologic analysis

Current Evidence and Applications of Photodynamic Therapy in Dermatology

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The Expressions of TGF-β₁and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment

The Expressions of TGF-β₁and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment