Ultrastructural colocalization of tyrosinated and detyrosinated alpha-tubulin in interphase and mitotic cells.

Geuens, G.; Gundersen, Gregg G.; Nuydens, Rony; Cornelissen, Frans W.; Bulinski, J. Chloë; DeBrabander, M

doi:10.1083/jcb.103.5.1883

Cited by 111 publications

(72 citation statements)

References 20 publications

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“…1, short detyr-tubulin sections of up to 1 mm long were disrupted by longer segments of tyr-tubulin, thus leading to a string-of-pearls-like appearance of the two tubulin variants with a preferential concentration of tyr-tubulin at the tubulus ends. Similar arrangements of interspersed detyr-and tyr-tubulin stretches had been seen for interphase and metaphase microtubules using immuno-EM (Geuens et al, 1986). However, antibodies directed against a-tubulin as a control were more or less evenly distributed along microtubules (supplementary material Fig.…”

Section: Distribution Of Detyrosinated and Tyrosinated Tubulin In Polsupporting

confidence: 61%

“…In MDCK cells, both the tyrosinated and detyrosinated isoforms were present along most of the length of double positive microtubules with alternating sections of the two isoforms. Segmental labelling of microtubules has been described previously (Geuens et al, 1986;Webster et al, 1987). Detyrtubulin stretches can be generated by an individual turnover from tyr-to detyr-tubulin, which is facilitated on pre-existing microtubules (Arce and Barra, 1985).…”

Section: Discussionmentioning

confidence: 99%

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Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells

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Große

Freikamp

et al. 2012

Journal of Cell Science

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SummaryThe role of post-translational tubulin modifications in the development and maintenance of a polarized epithelium is not well understood. We studied the balance between detyrosinated (detyr-) and tyrosinated (tyr-) tubulin in the formation of MDCK cell monolayers. Increased quantities of detyrosinated microtubules were detected during assembly into confluent cell sheets. These tubules were composed of alternating stretches of detyr-and tyr-tubulin. Constant induction of tubulin tyrosination, which decreased the levels of detyr-tubulin by overexpression of tubulin tyrosine ligase (TTL), disrupted monolayer establishment. Detyr-tubulin-depleted cells assembled into isolated islands and developed a prematurely polarized architecture. Thus, tubulin detyrosination is required for the morphological differentiation from non-polarized cells into an epithelial monolayer. Moreover, membrane trafficking, in particular to the apical domain, was slowed down in TTL-overexpressing cells. This effect could be reversed by TTL knockdown, which suggests that detyr-tubulin-enriched microtubules serve as cytoskeletal tracks to guide membrane cargo in polarized MDCK cells.

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Section: Distribution Of Detyrosinated and Tyrosinated Tubulin In Polsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells

Zink

Große

Freikamp

et al. 2012

Journal of Cell Science

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“…For example, it was shown that domains of acetylation occurred on stable MTs (Webster and Borisy, 1989), suggesting that this modification differentiated particular MT regions for specific functions. Similarly, detyrosination, which accumulates uniformly on MTs (Geuens et al, 1986), may mark entire MTs for functional specialization.…”

Section: Discussionmentioning

confidence: 99%

Detyrosination of alpha tubulin does not stabilize microtubules in vivo [published erratum appears in J Cell Biol 1990 Sep;111(3):1325-6]

Webster

Wehland

Weber

et al. 1990

The Journal of Cell Biology

141

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Abstract.The relationship between alpha tubulin detyrosination and microtubule (MT) stability was examined directly in cultured fibroblasts by experimentally converting the predominantly tyrosinated MT array to a detyrosinated (Glu) array and then assaying MT stability. MTs in mouse Swiss 3T3 cells displayed an increase in Glu immunostaining fluorescence ~1 h after microinjecting antibodies to the tyrosinating enzyme, tubulin tyrosine ligase. Detyrosination progressed to virtual completion after 12 h and persisted for 30-35 h before tyrosinated subunits within MTs were again detected. The stability of these experimentally detyrosinated MTs was tested by first injecting either biotinylated or Xrhodamine-labeled tubulin and then measuring bulk turnover by hapten-mediated immunocytochemistry or fluorescence recovery after photobleaching, respectively. By both methods, turnover was found to be similarly rapid, possessing a half time of ,'~ 3 min. As a final test of MT stability, the level of acetylated tubulin staining in antibody-injected cells was compared with that observed in adjacent, uninjected cells and also with the staining observed in cells whose MTs had been stabilized with taxol. Although intense Glu staining was observed in both injected and taxol-treated cells, increased acetylated tubulin staining was observed only in the taxol-stabilized MTs, indicating that the MTs were not stabilized by detyrosination. Together, these results demonstrated clearly that detyrosination does not directly confer stability on MTs. Therefore, the stable MTs observed in these and other cell lines must have arisen by another mechanism, and may have become posttranslationally modified after their stabilization.

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“…Detyrosination occurs after incorporation of tubulin subunits into the microtubule lattice (Kumar and Flavin, 1981) on diverse microtubules (Geuens et al, 1986;Gundersen and Bulinski, 1986;Gundersen et al, 1984;Johnson, 1998;Webster et al, 1987). Detyrosination is likely to be generated by the cytosolic carboxypeptidase 1 (CCP1, also known as NNA1), a finding based on the observation that a mutation in Ccp1 in mice greatly decreases the level of microtubule detyrosination (Kalinina et al, 2007).…”

Section: C-terminal Proteolytic Events Of -Tubulin -Detyrosination Amentioning

confidence: 99%

Post-translational modifications of microtubules

Włoga

Gaertig

2010

Journal of Cell Science

231

137

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There was an error published in J. Cell Sci. 123, 3447-3455.An incorrect version of Fig. 2 was published. The correct version, showing the severing of microtubules, is given below, together w its legend. We apologise for this mistake. CLIP-170Katanin, spastin There were errors published in J. Cell Sci. 123, 3447-3455.In Fig. 1, the arrows showing enzymatic reactions for MEC-17, HDAC6 and SIRT2 were pointing in the wrong direction. In addition, the C-terminal amino acid on the tail of -tubulin is Y (and not T). The correct figure is shown below.After publication of this Commentary, Shida and colleagues reported independent identification of MEC-17 homologs in diverse organisms as -tubulin acetyltransferases (Shida et al., 2010). Contrary to what we stated in our article and in the referenced paper (Akella et al., 2010), the newest version of the assembled genome of Chlamydomonas reinhardtii contains a sequence encoding an apparent homolog of the MEC-17 -tubulin acetyltransferase (locus ID Cre07.g345150).

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Ultrastructural colocalization of tyrosinated and detyrosinated alpha-tubulin in interphase and mitotic cells.

Cited by 111 publications

References 20 publications

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells

Detyrosination of alpha tubulin does not stabilize microtubules in vivo [published erratum appears in J Cell Biol 1990 Sep;111(3):1325-6]

Post-translational modifications of microtubules

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