Ultrastructural Cytochemistry of the Epidermis

Wolff-Schreiner, Elisabeth C.

doi:10.1111/j.1365-4362.1977.tb01835.x

Cited by 47 publications

(16 citation statements)

References 105 publications

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“…Higher in epidermis, these granules increase in number and size, move toward the distal cell membrane, fuse with the plasmalemma, and release their contents into the intercellular space. It has been postulated that these granules are responsible for the thickening of the stratum comeum cells and formation of the stratum comeum barrier (Wolff_Schreiner, 1977;Thompson and Hunt, 1966). Reduction in the number of these granules ( Fig.…”

Section: Discussionmentioning

confidence: 99%

DEPSCoR 99 Percutaneous Absorption, Skin Biophysics and Dermatotoxicity from JP-8 Jet Fuel

Singh¹

2003

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Section: Discussionmentioning

confidence: 99%

DEPSCoR 99 Percutaneous Absorption, Skin Biophysics and Dermatotoxicity from JP-8 Jet Fuel

Singh¹

2003

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“…Premature rat pups were obtained by cesarean section using isofluorane or halothane anesthesia at 18,19,20,21, and 22 (term) d EGA. Neonates were patted dry immediately after delivery and floated with their placental connections intact on a raft in a 36°C water bath.…”

Section: Methodsmentioning

confidence: 99%

Ontogeny of the Epidermal Barrier to Water Loss in the Rat: Correlation of Function with Stratum Corneum Structure and Lipid Content

et al. 1992

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ABSTRACT. The mammalian epidermal permeability barrier is provided by highly hydrophobic lipids forming multiple membrane bilayers within the extracellular domains of the outer, cornified cell layers. To characterize the critical events associated with barrier maturation, we correlated the emergence of a competent barrier to transepidermal water loss with development of the lamellar body secretory system, the organization of stratum corneum membrane bilayers, and the lipid composition of these membranes in the perinatal rat. The primary function of mammalian epidermis during terrestrial life is the generation and maintenance of a competent bamer

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“…Although the function of this 25K MCG-related protein is presently unknown, it is possible that it represents one of the several known MCG-associated enzymatic activities, including acid hydrolases and phospholipases, and several widely expressed lysosomal enzymes that have been histochemically localized to the intercellular space beneath the stratum corneum (Eisen et al, 1964;Elias et al, 1988;Frienkel and Traczyk, 1983;Gonzalez et al, 1976;Menon et al, 1986;Weinstock and Wilgrarn, 1970;Wolff-Schreiner, 1977). These MCG-associated enzymes are thought to be involved in a number of processes including the postsecretory modification ofMCG lipids converting them into a functional permobility barrier, and/or the degradation of intercellular constituents such as desmosomal components resulting in desquamation.…”

Section: Discussionmentioning

confidence: 99%

Association of a basic 25K protein with membrane coating granules of human epidermis.

O’Guin

Manabe

Sun

1989

The Journal of Cell Biology

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Abstract. Keratinocytes of the upper granular layers contain unique round-to-oval granules, 100-500 nm in diameter, in their peripheral cytoplasm. These granules (known as membrane coating granules [MCG], or lamellar granules) fuse with the apical cell surface of uppermost granular cells and discharge their contents into the intercellular space, where they are believed to play a role in establishing the permeability barrier of the epidermis and possibly in regulating the orderly desquamation of terminally differentiated keratinocytes. Using two monoclonal antibodies originally prepared against hair follicle antigens, we have identified a 25K epidermal protein in association with both MCG-like granules in the peripheral cytoplasm of granular cells as well as MCG-derived intercellular material. This protein is relatively basic (pI > 8), largely aqueous soluble, methionine deficient, and is relatively abundant in epidermis (comprising up to ",,0.1% of soluble proteins). Its distribution is restricted to the granular layer of keratinized (cornified) stratified squamous epithelia. The identification of this protein component opens new avenues for studying the molecular mechanisms underlying the establishment of permeability barrier and/or regulation of desquamation.

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Ultrastructural Cytochemistry of the Epidermis

Cited by 47 publications

References 105 publications

DEPSCoR 99 Percutaneous Absorption, Skin Biophysics and Dermatotoxicity from JP-8 Jet Fuel

DEPSCoR 99 Percutaneous Absorption, Skin Biophysics and Dermatotoxicity from JP-8 Jet Fuel

Ontogeny of the Epidermal Barrier to Water Loss in the Rat: Correlation of Function with Stratum Corneum Structure and Lipid Content

Association of a basic 25K protein with membrane coating granules of human epidermis.

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