Ultrastructural Effects of Cigarette Smoke on Rat Testis

Güven, Mustafa; Can, Belgin; Eyibilen, Ahmet; Saran, Yüksel; Aydos, Kaan

doi:10.1159/000020061

Cited by 22 publications

(5 citation statements)

References 28 publications

(21 reference statements)

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“…The lowest diameter of seminiferous tubules also can be found on the same experimental group with the dose of 4.0 mg/kg nicotine. Although the present study used nicotine, the fluctuations of seminiferous tubules diameter are similar to the results of Güven et al which found that exposure to nicotine, cigarette smoke and/or polycyclic aromatic hydrocarbons are able to produce testicular atrophy, block spermatogenesis and alter sperm morphologic features in rats (19).…”

Section: Discussionsupporting

confidence: 88%

The effect of nicotine per inhalation on the diameter and epithelium thickness of the seminiferous tubules of rats

En¹,

Primarizky²,

Widjiati³

et al. 2020

IJVS

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This study aimed to assess the level of testicular damage by observing the changes in the diameter and epithelium thickness of seminiferous tubules in rats that exposure to nicotine per inhalation. Thirty adult male rats were used and divided into five equal groups and treatment as follows for 20 days; Control group NaCl 0.9%, P1 nicotine 0.5 mg/kg, P2 nicotine 1.0 mg/kg, P3 nicotine 2.0 mg/kg and P4 nicotine 4.0 mg/kg. All groups were given treatment per inhalation for twenty days. At the end of treatment and the rats were sacrificed testes were collected for histopathological preparation. The testes were processed for routine paraffin embedding and staining and the sections were examined for histopathological changes. There results showed that nicotine administration induced varying degrees of structural damage to the seminiferous tubules, as the decreased in diamater and epithelium thickness of seminiferous tubules. The diameter and epithelium thickness of seminiferous tubules in four experimental groups reduced compared to the control group. This study proves that nicotine administration does decreases the spermatogenesis of rats by reducing the diameter and epithelium thickness of seminiferous tubules in testes. It also proves that the level of testicular damage is directly proportional to the dosage of nicotine administrated to male rats.

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Section: Discussionsupporting

confidence: 88%

The effect of nicotine per inhalation on the diameter and epithelium thickness of the seminiferous tubules of rats

En¹,

Primarizky²,

Widjiati³

et al. 2020

IJVS

View full text Add to dashboard Cite

show abstract

“…Therefore, during male puberty, serum AMH and testosterone levels show a significant negative correlation. 23,25,26 In our study, increased AMH and decreased inhibin B levels are suggested that ETS exposure has a inhibitor role on maturation of Sertoli cells. This inference is parallel with similar studies.…”

Section: Discussionsupporting

confidence: 58%

“…14 In a another study Guven et al reported that evident degeneration of the spermatogenetic cells and ultrastructural changing of the Sertoli cells were common findings in testicles from smoke exposed rats. 26 In addition to the Sertoli cells reduced testosterone levels may be related to the toxic effects of smoke on Leydig cells. In a previous study Yardimci et al investigated the effect of ETS exposure on the histology of Leydig cells in the testes of rats.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Environmental Tobacco Smoke on the Testicular Functions of 7-10 Years Old Children

Karaman¹,

Taş²,

Temel³

et al. 2012

JIUMF

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Purpose: To investigate the effect of environmental tobacco smoke (ETS) on the testicle functions of 7-10 years old children. Materials and Methods: The study included healthy male children aging between 7-10 years. Based on the questionnaire data, 50 passive smoking (PS) cases and 50 passive smoking free controls were determined. Serum levels of cotinine, and biochemical parameters of the testicle functions; testosterone, inhibin B, anti-mullerian hormone (AMH) along with FSH and LH levels were evaluated. Results: Serum cotinine levels in PS group were found higher than the controls (p<0,05). Testosterone, inhibin-B, FSH, and LH levels of the control group was found higher than the PS group. The AMH levels were found lower in the controls in comparison with the PS group (p<0,05). Conclusions: Our results showed that ETS leads to testicular dysfuntions. We think that preventing exposure to cigarette smoke in childhood is very important for their reproductive system.

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“…The differences between the control group production and suppresses spermatogenesis, thus directly leading to male infertility (6). Impaired spermatogenesis is also a result of the degeneration of spermatogenic and Sertoli cells due to nicotine (7).…”

Section: Discussionmentioning

confidence: 99%

Low-dose Nicotine Exposure Induced the Oxidative Damage of Reproductive Organs and Altered the Sperm Characteristics of Adolescent Male Rats

Budin

Kho

Lee

et al. 2017

MJMS

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Background: Nicotine is a major toxic and hazardous component of cigarette smoke, and it has been widely used in nicotine replacement therapy (NRT). This study was aimed to investigate the effects of chronic low-dose nicotine on sperm characteristics and reproductive organ integrity in adolescent male Sprague-Dawley rats.Methods: Twelve rats were equally divided into two groups. Group I received normal saline, and group II received 0.6 mg/kg body weight nicotine intraperitoneally for 28 consecutive days. At the end of the experimental period, sperm was collected for sperm characteristic evaluation, and the testes and prostate were isolated for biochemical and morphological analysis. The effects of nicotine on the body and reproductive organ weights of the animals were evaluated.Results: Chronic nicotine treatment significantly (P < 0.05) altered the sperm count, motility, viability, and morphology, and remarkably increased the malondialdehyde (P < 0.001) and advanced oxidation protein product (P < 0.05) levels in the testes and prostate of nicotinetreated group compared to control group. Moreover, nicotine caused a significant decrease (P < 0.05) in the superoxide dismutase activity of the testes. No significant differences were observed in the reduced glutathione level in both of the testes and prostate of nicotine group compared with control group. Nicotine also induced histopathological alteration in the testes.Conclusion: A low-dose nicotine exposure at 0.6 mg/kg caused detrimental effects on sperm characteristics and induced oxidative stress in the testes and prostate.

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Ultrastructural Effects of Cigarette Smoke on Rat Testis

Cited by 22 publications

References 28 publications

The effect of nicotine per inhalation on the diameter and epithelium thickness of the seminiferous tubules of rats

The effect of nicotine per inhalation on the diameter and epithelium thickness of the seminiferous tubules of rats

The Effect of Environmental Tobacco Smoke on the Testicular Functions of 7-10 Years Old Children

Low-dose Nicotine Exposure Induced the Oxidative Damage of Reproductive Organs and Altered the Sperm Characteristics of Adolescent Male Rats

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