Ultrastructural localization of fibroblast growth factor in neurons of rat brain

Expression of acidic and basic fibroblast growth factors in the substantia nigra of rat, monkey, and human.

“…Northern analysis has suggested that bFGF mRNA is distributed widely throughout the brain (35), and immunohistochemical studies have reported localization of bFGF in neurons of the cerebral cortex, hippocampus, cerebellum (36)(37)(38), and in neuroglia (39). These data are in accord with our present observations suggesting a widespread distribution of bFGF in neurons and glial cells within brain.…”

Localization of basic fibroblast growth factor (bFGF)-like immunoreactivity in neual circuits innervating the gastrocnemius muscle, with reference to the direction of bFGF transport.

“…This may be accounted for by the fact that bFGF-immunoreactive structures, even though heterogeneously distributed in the cytoplasm in 60 nm ultrathin sections, show a diffuse distribution pattern within the cell body in a 60 am section as a result of the overlapping of individual immunoreaction products. The use of 0.1 M PBS with a higher osmolarity than the ordinary incubation medium for immunostaining may also have made antigenantibody binding more specific in this study (IwATA et al,1991;MATSUDA et al, 1992a, b), thereby localizing immunoreaction deposits in more discrete cytoplasmic areas than in the study of JANET et al (1987). Moreover, a small amount of hydrogen peroxide produced byQ-D glucose catalysis in the final incubation seems to have served to maintain tissue ultrastructures and thus may have enabled us to identify the locations of bFGF-immunoreactivity more precisely than before (SAKANAKA et al, 1987).…”

“…Previous immunohistochemical studies demonstrated that bFGF is present in neurons (PETTMANN et al, 1986;JANET et al, 1987;GROTHE et al, 1991) and astrocytes (GOMEZ-PINILLA et al, 1992) and in certain peripheral tissues such as muscle, the adrenal gland, ovary and pituitary (DIMARIO et al, 1989;GROTHE and UNSICKER, 1989;JOSEPH-SILVERSTEIN et al, 1989;KARDAMI and FANDRICH,1989;MATSUDA et al, 1992b). A recent study at our laboratory also showed bFGF-like immunoreactivity in the cell bodies and peripheral processes of trigeminal mesencephalic and motor nucleus neurons, suggesting that central bFGF is anterogradely transported towards the muscles of mastication (MATSU-DA et al, 1992a;DESAKI et al, 1992).…”

Immunoelectron Microscopic Localization of Basic FGF in Neuroglias and Neurons of the Trigeminal Mesencephalic and Motor nuclei

“…One explanation for the discrepancy between the above immunohistochemical studies is that they employed different antibodies against bFGF. However, reexamination of bFGF immunoreactivity in the normal brain with a bFGF antiserum that has been proved to recognize exclusively neuronal bFGF, if it showed the presence of bFGF in neuroglial cells, would reconcile the results of GOmez-Pinilla et al (1992) with those of previous studies that suggest bFGF is located in neurons but not in neuroglias (Pettman et al 1986, Janet et al 1987, Janet et al 1988, Grothe et al 1991, Matsuda et al 1992a). The present study correlated light and electron microscopic findings in order to re-examine the localization of bFGF immunoreaction in neuroglias and neurons of the rat mesencephalic and motor trigeminal nuclei, * To whom all correspondence should be addressed .…”

“…It facilitates the survival and neurite extension of neurons cultured from the embryonic rat cortex, fetal rat hippocampus, newborn rat cortex, ciliary ganglion, fetal mesencephalon, adult rat retina and chick spinal cord (Walicke et al 1986, Morrison et al 1986, Gensburger et al 1987, Schubert et al 1987, Unsicker et al 1987, Walicke 1988, Bahr et al 1989, Ferrari et al 1989. In support of a trophic or protective effect of bFGF on neurons, previous immunohistochemical studies have demonstrated bFGF-like molecules exclusively in neurons (Pettman et al 1986, Janet et al 1987, Janet et al 1988, Grothe et al 1991). More recently, GOrnezPinilla et al (1992) have reported the presence of bFGF not only in neurons of discrete brain nuclei but also in neuroglial cells under normal and pathologic conditions.…”