Ultrastructural localization of laminin-5 (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" altimg="si1.gif" overflow="scroll"><mml:mrow><mml:mi>γ</mml:mi><mml:mn>2</mml:mn></mml:mrow></mml:math> chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy

Atsuta, Ikiru; Yamaza, Takayoshi; Yoshinari, Masao; Goto, Tetsuya; Kido, Mizuho A.; Kagiya, Tadayoshi; Mino, Satoya; Shimono, Masaki; Takano, Teruo

doi:10.1016/j.biomaterials.2005.03.046

Cited by 101 publications

(131 citation statements)

References 30 publications

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“…Previous studies have discovered a basement membrane-like, laminin-5-rich layer interposed between epithelial cells and titanium [4,9]. In addition, HSPG, a proteoglycan found at high levels in blood plasma, is known to bind to both Ca 2?…”

Section: Discussionmentioning

confidence: 99%

“…In some rodent oral implant studies, epithelial attachment structures such as HDs or basement membrane are of inferior quality and quantity [4,9] to that observed around a natural tooth. It has been reported that peri-implant connective tissue attachments consisting of fibroblasts and collagen are similar to scar tissue, and have less cells than healthy periodontium [10].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

et al. 2012

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In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium plates hydrothermally-treated with CaCl2 (Ca) or MgCl2 (Mg) solution, or distilled water (DW). The number and adhesive strengths of attached cells on the substrata were then analyzed. The number of Sa3 cells adhering to the Ca- and Mg-treated plates was significantly larger than in the DW group, but the strength of this adhesion did not differ significantly between groups. In contrast, NIH3T3 cell adhesion number and strength were increased in both the Ca and Mg groups compared to the DW group. Fluorescent microscopic observation indicated that, in all groups, Sa3 had identical expression levels of integrin β4 and development of actin filaments, whereas NIH3T3 cells in the Ca and Mg groups displayed much stronger punctate cytoplasmic signals for vinculin and more bundle-shaped actin filaments than cells in the DW group. As a result, it was indicated that the hydrothermal treatment of titanium with Ca or Mg solution improved the integration of soft tissue cells with the substrata, which may facilitate the development of a soft tissue barrier around the implant.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

et al. 2012

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“…Around natural teeth, the junctional epithelium attaches to the tooth by hemidesmosomes formed by laminin γ2 and integrin β4 via the internal basal lamina, acting as a biological seal between the oral environment and the internal structures of the body. On the other hand, it has been reported that the peri-implant epithelium attaches only weakly at the apical portion of peri-implant epithelium-implant surface made from Ti by laminin γ2 in rat 9) . This means that inflammation around implant can occur more easily than around natural teeth.…”

Section: Introductionmentioning

confidence: 96%

Initial attachment of human oral keratinocytes cultured on zirconia or titanium

et al. 2012

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Initial attachment of human oral keratinocytes (HOKs) cultured on mirror-surfaced commercially pure titanium (Ti) or yttriastabilized tetragonal zirconia polycrystals (TZP) was investigated. Numbers of viable attached HOKs, their mRNAs and proteins expression of laminin γ2 and integrin β4 were evaluated using the WST-1 assay, quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Localization of laminin γ2 and integrin β4 was observed using immunofluorescent staining. Cell spreading was evaluated by measuring the perimeter of actin on fluorescent stained images, and cell morphology was examined using scanning electron microscopy. At 1 h TZP elicited less of initial attachment than Ti in terms of mRNAs expression and proteins expression of laminin γ2 and integrin β4 (p<0.05). However, at 48 h TZP was showed similar initial attachment in comparison to Ti. Therefore, it was suggested that TZP has a potential to form epithelial attachment like Ti.

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“…The soft tissue barrier around dental implants serves as a protective seal between the oral environment and the underlying bone. However, it has been reported that the peri-implant epithelium attaches only weakly at the apical portion of peri-implant epithelium-implant surfaces made from Ti by laminin γ 2 in rat 6) . This indicates that peri-implant epithelium may not function as a tight biological seal against the invasion of foreign material 7) .…”

Section: Introductionmentioning

confidence: 97%

Influence of plasma and ultraviolet treatment of zirconia on initial attachment of human oral keratinocytes: Expressions of laminin γ2 and integrin β4

et al. 2014

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Initial attachment of human oral keratinocytes cultured on yttria-stabilized tetragonal zirconia polycrystal (TZP) surfaces that were subjected to UV or oxygen plasma (O2-plasma) treatment was investigated. The viability of the attached cells, mRNA expression of laminin γ2 and integrin β4, distribution of laminin γ2 and integrin β4, cell area, and cell morphology were assessed. The results showed that no differences in the viability of attached cells were recognized among the conditions. However, expression of laminin γ2 and integrin β4 as well as cell morphology were promoted only in O2-plasma specimens even though superhydrophilicity was obtained in both the UV and O2-plasma specimens compared with the untreated control specimen. The photocatalytic activity was believed to be closely involved in the above-mentioned differences. The results of this study suggest that TZP surface treated with oxygen plasma promotes the initial attachment capability of human oral keratinocytes with enhancing the extracellular matrix such as laminin γ2.

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Ultrastructural localization of laminin-5 (γ2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy

Cited by 101 publications

References 30 publications

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

Effect of Titanium Surface Calcium and Magnesium on Adhesive Activity of Epithelial-Like Cells and Fibroblasts

Initial attachment of human oral keratinocytes cultured on zirconia or titanium

Influence of plasma and ultraviolet treatment of zirconia on initial attachment of human oral keratinocytes: Expressions of laminin γ2 and integrin β4

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