Ultrastructural localization of rDNA and rRNA by in situ hybridization in the nucleolus of human spermatids

Dadoune, Jean‐Pierre; Siffroi, Jean‐Pierre; Alfonsi, Marie-Françoise

doi:10.1007/bf00331381

Cited by 14 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…, 1992. Similar results were obtained in human spermatocytes , human spermatids (Dadoune et al, 1994). and rat liver nucleoli (Jimenez-Garcia et al, 1993).…”

supporting

confidence: 74%

Localization of ribosomal genes in human spermatogonia by fluorescent in situ hybridization and confocal microscopy

Lévy

Navarro²,

Depétris³

et al. 1996

Cytogenet Genome Res

View full text Add to dashboard Cite

The distribution of ribosomal RNA genes in nucleoli is still a matter of controversy. We have investigated the nucleolus of human type A spermatogonia, which displays a single, large fibrillar center. Silver-staining was used to localize the fibrillar center by light microscopy. Fluorescent in situ hybridization was performed on the same cell, using a 5.8-kb probe specific for a transcribed region of the ribosomal genes. The fluorescent area exactly corresponded to the silver-stained area. The three-dimensional distribution of rDNA was studied in horizontal and orthogonal sections using a confocal laser scanning microscope. The presence of the fluorescent signal throughout the whole silver-stained structure demonstrated that the fibrillar center, and at least a part of the dense fibrillar component, contained most of the rDNA.

show abstract

“…, 1992. Similar results were obtained in human spermatocytes , human spermatids (Dadoune et al, 1994). and rat liver nucleoli (Jimenez-Garcia et al, 1993).…”

supporting

confidence: 74%

Localization of ribosomal genes in human spermatogonia by fluorescent in situ hybridization and confocal microscopy

Lévy

Navarro²,

Depétris³

et al. 1996

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…Since the introduction of in situ hybridization at the EM level with rDNA probes, the precise intranucleolar localization of rDNA genes in untreated cells has been subject to dispute: one group of authors reports that only the fibrillar centres house the rDNA genes in Ehrlich tumour cells and HeLa cells (Thiry & ThiryBlaise 1989, Puvion-Dutilleul et al 1991. Another group, including ourselves, located ribosomal genes in the dense fibrillar component of human lymphocytes and human testicular cells (Wachtler et al 1989, Hoz~k et al 1993, Dadoune et al 1994 and rat liver cells (Jimenez-Garcia et al 1993).…”

Section: Discussionmentioning

confidence: 97%

Redistribution of ribosomal DNA after blocking of transcription induced by actinomycin D

et al. 1996

View full text Add to dashboard Cite

We report on the effect of different doses and times of incubation of the cytostatic drug actinomycin D (AMD) on nucleolar morphology, rRNA gene transcription and rDNA gene localization using in situ hybridization and the immunocytochemical detection of the human upstream binding factor (UBF) at the electron microscopic level in HeLa cells. Low doses of AMD (0.001 micrograms/ml, 30 min) selectively block rRNA gene transcription but alter neither nucleolar morphology nor the localization of rDNA with respect to the nucleolar components. Treatment with high doses of AMD (0.05 micrograms/ml, 1 h) resulted in a retraction of the rDNA out of the nucleolus in addition to the well-known blocking of rDNA transcription, total nuclear transcription and nucleolar segregation. Under these conditions accumulations of rDNA were found in patches of chromatin at the nucleolar periphery. We conclude that the blocking of rRNA gene transcription and the changes in nucleolar morphology, both induced by AMD at different doses, are independent phenomena.

show abstract

“…In this instance rDNA is found almost exclusively in the DF, but not in the large and (in most cases) single FC, with the exception of weak signals in its periphery in close connection with the DF Dadoune et al, 1994). In human spermatids that have a small but still active reticulated nucleolus the rRNA genes are found in the DF which builds up the reticulum (Dadoune et al, 1994). In human spermatogonia A the rRNA genes were again found rather in the FC but also in part in the small surrounding DF as shown by comparison with silver staining (Levy et al, 1996).…”

Section: Studies On Intact Nucleoli In Situmentioning

confidence: 99%

Ribosome biogenesis in man: Current views on nucleolar structures and function

Schwarzacher¹,

Mosgoeller²

2000

Cytogenet Genome Res

View full text Add to dashboard Cite

Nucleoli develop when preribosomes are synthesized at the chromosomal nucleolar organizer regions. Typically they consist of at least three nucleolar subcompartments, the fibrillar center (FC), the dense fibrillar component (DF), and the granular component (GC). The understanding of the functional arrangements of these subcompartments relates to aspects in cell biology, pathology, and virus research. In the present review morphological studies are discussed in the light of molecular findings. The available data confirm the hypothesis that rDNA transcription is connected with the DF but not necessarily with the presence of an FC. Within the DF, rDNA transcription is restricted to foci, possibly representing single transcribing genes. FCs may serve to store inactive transcription factors, to initiate rDNA transcription, and may provide structural support for transcription. The GC can be interpreted as a collection of maturing preribosomes. More recently the nucleolar subcompartments were focused on in the context of virus research and tumor biology.

show abstract

Ultrastructural localization of rDNA and rRNA by in situ hybridization in the nucleolus of human spermatids

Cited by 14 publications

References 21 publications

Localization of ribosomal genes in human spermatogonia by fluorescent in situ hybridization and confocal microscopy

Localization of ribosomal genes in human spermatogonia by fluorescent in situ hybridization and confocal microscopy

Redistribution of ribosomal DNA after blocking of transcription induced by actinomycin D

Ribosome biogenesis in man: Current views on nucleolar structures and function

Contact Info

Product

Resources

About