Ultrastructural localization of γ‐glutamyl transpeptidase in rat kidney and jejunum

Marathe, Gopal V.; Nash, Barbara; Haschemeyer, Rudy H.; Tate, Suresh S.

doi:10.1016/0014-5793(79)80425-1

Cited by 86 publications

(27 citation statements)

References 25 publications

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“…Studies have revealed that ischemia induced swelling and morphological changes in the proximal tubules of kidneys in rats [12]. This process was associated with increased GGT activity and decreased synthesis of GSH [13,14].…”

Section: Discussionmentioning

confidence: 99%

Gamma-Glutamyl Transpeptidase as the Marker of Kidney Graft Function

Kwiatkowska¹,

Domański²,

Bober³

et al. 2014

Adv Clin Exp Med

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Background. Gamma-glutamyl transpeptidase (GGT) is a glycoprotein of the external surface of various cell types. The activity of GGT has been observed in cells and tissues with secretory activity, such as the proximal tubular cells in kidneys. GGT also plays an important pro-oxidant role, stimulating the generation of hydroxyl radicals, and increases membrane lipid peroxidation. Objectives. In this study we examined whether the monitoring of GGT activity in urine may be a prognostic factor of kidney allograft function. The study enrolled 107 Caucasian renal transplant recipients. Material and Methods. Urine samples were collected for GGT and creatinine analysis on the 1 st day after transplantation, and then in the 3 rd and 12 th month. Results. Higher urine GGT activity in the 3 rd month after transplantation was associated with significantly higher risk of graft failure (HR = 1.063 per each U/g creatinine; 95%Cl:1.004-1.127; p = 0.037) in the Cox proportional hazards model. Moreover, there were positive correlations between urine GGT and the grade of interstitial fibrosis (Rs = 0.64, p = 0.01) and tubular atrophy (Rs = 0.54, p = 0.056) in specimens collected in the 3 rd month after transplantation. Conclusions. Our results suggest that higher urine GGT activity in the 3 rd month after transplantation may be a prognostic factor of graft failure (Adv Clin Exp Med 2014, 23, 6, 947-952).

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Section: Discussionmentioning

confidence: 99%

Gamma-Glutamyl Transpeptidase as the Marker of Kidney Graft Function

Kwiatkowska¹,

Domański²,

Bober³

et al. 2014

Adv Clin Exp Med

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show abstract

“…Introduction yGlutamy1 transpeptidase catalyzes the initial step in the breakdown of glutathione. A proposed role for the enzyme in transport processes [ 1,2] is consistent with its localization in the plasma membranes of a variety of epithelial cells [3,4]. The enzyme is also present in human and rat lymphoid cells and the activity can be increased by treatment of the cells with mitogenic agents [5].…”

mentioning

confidence: 89%

“…Ultrastructural localization of the transpeptidase activity in lymphoid cells was done as in [4,10]. Washed cells (-5 X 10') were mixed with 0.2 ml melted agar (2% in phosphate-NaCl) at 45°C.…”

Section: Elsevierjnorth-holland Biomedical Pressmentioning

confidence: 99%

“…In controls, the transpeptidase activity was inhibited by the addition of L-serine and borate (5 mM each) to the incubation medium [ 111. Osmication, dehydration, and embedding in Araldite was done as in [4]. Ultrathin sections were cut on a LKB ultramicrotome and observed in a JEM-100B electron microscope.…”

Section: Elsevierjnorth-holland Biomedical Pressmentioning

confidence: 99%

“…Lymphoid cells were treated with the ferritin conjugate of anti-human kidney transpeptidase IgG as in [4]. Cells were prefuted with 1% formaldehyde in phosphate-NaCl for 30 min at 4°C.…”

Section: Elsevierjnorth-holland Biomedical Pressmentioning

confidence: 99%

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