Ultrastructural stereologic analysis of cardiomyocytes in spontaneously hypertensive rats

Лушникова, Е. Л.; Nepomniashchikh, G I; Туманов, В. П.; Непомнящих, Л. М.; Gonchar, A. M.

doi:10.1007/bf00831247

Cited by 2 publications

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“…The revealed intracellular reorganization of CMC is similar to that observed in spontaneously hypertensive rats during the development of CMC hypertrophy [2,4]. It is possible that this dynamics of quantitative and qualitative changes in the main sarcoplasmic organelles of CMC reflects general principles of their spatial and temporal reorganization underlying hypertrophic changes of different genesis.…”

Section: Resultsmentioning

confidence: 59%

“…Similar changes in the energy-producing and contractile compartments of CMC were observed in various experimental models of myocardium hypertrophy [2,4,[14][15][16] and can be regarded as a sequence of adaptive reactions. CMC hypertrophy in 3-and 10-month-old W/SSM rats was accompanied by a significant decrease in the volume (by 35 and 23%, respectively) and surface (by 35 and 42%, respectively) density of SSR.…”

Section: Resultsmentioning

confidence: 71%

“…Ultrastructural study of CMC of 3-month-old W/ SSM rats reveal no significant differences in comparison with age-matched Wistar rats and spontaneously hypertensive rats (SHR) [2]. Myofibrillar bundles and mitochondria were compactly arranged in cells ( Fig.…”

Section: Resultsmentioning

confidence: 98%

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Intracellular reorganization and ultrastructural stereological analysis of cardiomyocytes of W/SSM rats with genetically determined cardiomyopathy

et al. 1999

Bull Exp Biol Med

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We studied ultrastructure of cardiomyocytes of W/SSM rats with inherited hypertrophic cardiomyopathy bred from Wistar rats by hypersensitivity to cataractogenic action of high galactose doses. The observed dynamics of quantitative and qualitative changes in the main sarcoplasmic organelles reflects general principles of spatial and temporal reorganization of cardiac muscle cells induced by hypertrophy of different genesis.Key Words: W/SSM rats; hypertrophic cardiomyopathy; cardiomyocytes; electron microscopy; morphometry; stereology W/SSM rats with inherited hypertrophic cardiomyopathy were bred from Wistar rats selected by hypersensitivity to cataractogenic action of high galactose doses at the Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science [8,12,13]. These animals are characterized by spontaneous myocardium hypertrophy accompanied by diffuse stroma collagenization, focal sclerotic changes, and chronic cardiac insufficiency [6,7,9].One of the mechanisms of pathologic process is based on changes in plasma membrane integrity resulting from enhanced hexose transport into the cells, generation of free hydroxyradicals, and intensification of lipid peroxidation [ 1,17]. In the myocardium of W/ SSM rats with genetically determined disturbances of the carbohydrate metabolism and increased lysosome Thus, hypertrophic changes in the myocardium of W/SSM rats are similar to those observed during idiopathic human hypertrophic cardiomyopathy in the absence of anatomic or physiologic causes [11].The aim of the present study was to reveal intracellular changes in CMC and to determine ultrastructural stereological parameters typical of W/SSM rats with genetically determined hypertrophic cardiomyopathy. MATERIALS AND METHODSForty male W/SSM rats aged 3 and 10 months and 20 -male Wistar rats of the same age kept under standard vivarium conditions were used in the experiment. The animals were weighed and decapitated under chloro-

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Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 71%

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Intracellular reorganization and ultrastructural stereological analysis of cardiomyocytes of W/SSM rats with genetically determined cardiomyopathy

et al. 1999

Bull Exp Biol Med

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“…Myocardium hypertrophy in these animals develops at an early age and has been shown not to be directly related to the development of arterial hypertension [8,9]. Morphogenesis of myocardium hypertrophy, the pattern of ultrastructural changes in cardiomyocytes, and the spatial tissue and intracellular rearrangement of the myocardium in SHR are similar in many features to the morphological rnardfestation of hypertrophic cardiomyopathy in man and have been described at length [1,[3][4][5]. However, the causes of the development of myocardium hypertrophy in SHR remain quite uncertain; in particular, little is known about the ratio between proliferative processes (or hyperplasty) and hypertrophy of cardiomyocytes in early ontogeny and their contribution to myocardium hypertrophy in SHR.…”

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confidence: 93%

“…Attention has been focused on the effect of hyperthermia on cultured cells, predominantly on tumor cells [1,11]. As a result of these studies, hyperthermia has found application in anticancer therapy, since tumor cells have a low resistance to heating.…”

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confidence: 99%

Cellular mechanisms of hypertrophic cardiomyopathy in spontaneously hypertensive rats (SHR)

Семенов

Nepomnyashchikh

1994

Bull Exp Biol Med

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It is shown that the weight dynamics of the body, heart and left ventricular myocardium is similar in Wistar rats and SHR, but the number of cardiomyocytes in the 5th and 24th weeks of postnatal development is considerably higher in Wistar rats than in SHR. The earlier maturation of cardiomyocytes in SHR rats leads to their reduced proliferative activity, thus blocking the growth of the cell population. Key Words: cardiomyocytes; hypertrophy; spontaneously hypertensive rats (StfR); cell population sizeModeling of hereditary cardiomyopathy is of great importance for elucidating the general pathways of heart pathology in man. Hypertrophic cardiomyopathy in spontaneously hypertensive rats (SHR) is considered to be one such model [6,10,12,13]. Myocardium hypertrophy in these animals develops at an early age and has been shown not to be directly related to the development of arterial hypertension [8,9]. Morphogenesis of myocardium hypertrophy, the pattern of ultrastructural changes in cardiomyocytes, and the spatial tissue and intracellular rearrangement of the myocardium in SHR are similar in many features to the morphological rnardfestation of hypertrophic cardiomyopathy in man and have been described at length [1,[3][4][5]. However, the causes of the development of myocardium hypertrophy in SHR remain quite uncertain; in particular, little is known about the ratio between proliferative processes (or hyperplasty) and hypertrophy of cardiomyocytes in early ontogeny and their contribution to myocardium hypertrophy in SHR.The objective of the present investigation was to compare the dynamics of cardiomyocyte population growth in postnatal ontogeny in Wistar rats and SHR. MATERIALS AND METHODSThirty-two Wistar rats (controls) and 21 SHR (both 1 day old) were selected for the experiments. Ten Wistar rats and 9 SHR were killed at the start point and the others after attaining the 5-or 24-week age. All the animals were weighed before sacrifice. The hearts were fixed by perfusion with 10% paraformaldehyde in 0.1 M phosphate buffer saline (pH 7.2) until the myocardium was completely washed free of blood. After the atrii were removed, the myocardium of both ventricles (total heart weight) and of the left ventricle with the interventricular septum were weighed. Alkaline dissociation of the fixed myocardium was performed according to a standard procedure [7].

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Ultrastructural stereologic analysis of cardiomyocytes in spontaneously hypertensive rats

Cited by 2 publications

References 4 publications

Intracellular reorganization and ultrastructural stereological analysis of cardiomyocytes of W/SSM rats with genetically determined cardiomyopathy

Intracellular reorganization and ultrastructural stereological analysis of cardiomyocytes of W/SSM rats with genetically determined cardiomyopathy

Cellular mechanisms of hypertrophic cardiomyopathy in spontaneously hypertensive rats (SHR)

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