Ultrastructural studies of the in vitro chitin and cuticle secretions in the wing pads of <i>Locusta</i>

Cassier, Pierre; Papillon, M.

doi:10.1139/z91-005

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…Among them are the phyto‐derived from Meliaceae family, including A. indica (neem)—which has azadirachtin as main active principle. Studies on insects control using this product have demonstrated its effect on the oviposition and fecundity of individuals exposed to it, with consequent interference in the reproduction (Copping and Menn,2000; Isman,1997).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory action of neem aqueous extract (azadirachta indica A. Juss) on the vitellogenesis of rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) ticks

Denardi

Bechara

Oliveira

et al. 2010

Microscopy Res & Technique

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The present study revealed unheard of data about the action of aqueous extracts of neem leaves (Azadirachta indica) on the vitellogenesis of Rhipicephalus sanguineus ticks, proving that these extracts in 10 and 20% concentrations do not have the potential to kill the females; however, in lower concentrations (10%) provokes great morphological alterations in germinative cells such as the emergence of extended cytoplasmic vacuolization areas as well as the fragmentation of the germinal vesicle, even in those oocytes which were in initial stages of development (I-III), showing that neem is a potent agent which acts impeding one of the main metabolic stages of the ticks, i.e., the reproduction. In oocytes in final stages of development (IV-V) azadirachtin (neem's active principle) caused significant reduction in the size and quantity of proteic granules of the yolk and the inversion of their localization where the smaller granules before inside the cell (normal oocyte) were posteriorly observed in the periphery, and the bigger ones in the central region. Thus, the study showed that the alterations found both in the oocytes and in the pedicel cells indicated that azadirachtin acts on the process of tick's reproduction and signalizes that this plant can be used in the future to control ticks with the advantage of not being aggressive to nontarget organism or the environment. Furthermore, data here obtained showed that the most significant efficiency of the aqueous extract of neem is related to the concentration of 10%, proving that higher doses would not be so efficient.

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Section: Discussionmentioning

confidence: 99%

Inhibitory action of neem aqueous extract (azadirachta indica A. Juss) on the vitellogenesis of rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) ticks

Denardi

Bechara

Oliveira

et al. 2010

Microscopy Res & Technique

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“…Essa substância, não necessariamente, age matando diretamente o inseto, como a maioria dos inseticidas; atua sobre a reprodução e crescimento ou como toxina direta (ROY;SARAF, 2006). Ao que se sabe, um dos mecanismos de ação mais comum da azadiractina é por meio da inibição da síntese de quitina necessária para o desenvolvimento dos insetos (CASSIER;PAPILLON, 1991).…”

Section: Discussionunclassified

Atividade inseticida in vitro do óleo de sementes de nim sobre Lutzomyia longipalpis (Diptera: Psychodidae)

Maciel¹,

Morais²,

Beviláqua³

et al. 2010

RBPV

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Lutzomyia longipalpis is the main vector of visceral leishmaniasis in Brazil. The objective was to evaluate the effect of oil from (Azadirachta indica) neem seeds on eggs, larvae and adults of the vector. The insects were captured in the field and kept in the laboratory at +/- 27 °C and 80% relative humidity. Five treatments with different concentrations were performed using two negative controls (distilled water and Tween 80) and a positive control. The eggs were sprayed with the oil at different concentrations and the number of hatched larvae evaluated for 10 days. Mortality of larvae was observed to pupation and adult mortality was observed after 24, 48, and 72 hours. Statistical analysis was performed by Tukey test at 5% probability. The highest oil concentration of eggs obtained 65.16 +/- 3.24% efficacy for reducing egg hatching. The test with larvae showed 67.75 +/- 2.21% efficacy at a concentration of 100 mg.mL⁻¹. In adults, the efficacy of the 100 mg.mL⁻¹ concentration was 96.64 +/- 4.11% after 24 hours. The phytochemical analysis revealed the presence of triterpenes. These results demonstrate the potential use of this oil in the control of this vector.

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Morphogenesis of the antenna of the male silkmoth. Antheraea polyphemus, III. Development of olfactory sensilla and the properties of hair-forming cells

Keil

Steiner

1991

Tissue and Cell

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Ultrastructural studies of the in vitro chitin and cuticle secretions in the wing pads of Locusta

Cited by 7 publications

References 20 publications

Inhibitory action of neem aqueous extract (azadirachta indica A. Juss) on the vitellogenesis of rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) ticks

Inhibitory action of neem aqueous extract (azadirachta indica A. Juss) on the vitellogenesis of rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) ticks

Atividade inseticida in vitro do óleo de sementes de nim sobre Lutzomyia longipalpis (Diptera: Psychodidae)

Morphogenesis of the antenna of the male silkmoth. Antheraea polyphemus, III. Development of olfactory sensilla and the properties of hair-forming cells

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